Zobrazeno 1 - 10
of 37
pro vyhledávání: '"Rachel J Skilton"'
Autor:
Morgan R Herod, Omar Salim, Rachel J Skilton, Cynthia A Prince, Vernon K Ward, Paul R Lambden, Ian N Clarke
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e90679 (2014)
Investigations into human norovirus infection, replication and pathogenesis, as well as the development of potential antiviral agents, have been restricted by the lack of a cell culture system for human norovirus. To date, the optimal cell culture su
Externí odkaz:
https://doaj.org/article/2bb8d2b4de25413f84d7066b538b8b0e
Autor:
Yibing Wang, Simona Kahane, Lesley T Cutcliffe, Rachel J Skilton, Paul R Lambden, Kenneth Persson, Carina Bjartling, Ian N Clarke
Publikováno v:
PLoS ONE, Vol 8, Iss 3, p e59195 (2013)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (r
Externí odkaz:
https://doaj.org/article/3fbc15f3ffdf44f8ad7a56d3d7a12204
Autor:
Clare eLabiran, Ian N Clarke, Lesley T Cutcliffe, Yibing eWang, Rachel J Skilton, Kenneth ePersson, Carina eBjartling, Björn eHerrmann, Linus eChristerson, Peter eMarsh
Publikováno v:
Frontiers in Cellular and Infection Microbiology, Vol 2 (2012)
We aimed to evaluate the stability of the Chlamydia trachomatis MLVA-ompA and MST typing systems through multiple passages in tissue culture. Firstly, we analysed the stability of these markers through adaptation of C.trachomatis to tissue culture an
Externí odkaz:
https://doaj.org/article/fc70c4b532a74ad9ad39b37d4c1a2a65
Autor:
Yibing Wang, Simona Kahane, Lesley T Cutcliffe, Rachel J Skilton, Paul R Lambden, Ian N Clarke
Publikováno v:
PLoS Pathogens, Vol 7, Iss 9, p e1002258 (2011)
Chlamydia trachomatis remains one of the few major human pathogens for which there is no transformation system. C. trachomatis has a unique obligate intracellular developmental cycle. The extracellular infectious elementary body (EB) is an infectious
Externí odkaz:
https://doaj.org/article/2958f515e06044beabff360594a39a65
Publikováno v:
PLoS ONE, Vol 6, Iss 2, p e16971 (2011)
Genital chlamydia infection is the most commonly diagnosed sexually transmitted infection in the UK. C. trachomatis genital infections are usually caused by strains which fall into two pathovars: lymphogranuloma venereum (LGV) and the genitourinary g
Externí odkaz:
https://doaj.org/article/bd01f73511994d54a92d92b29b0d4532
Autor:
Rachel J Skilton, Lesley T Cutcliffen, David Barlow, Yibing Wang, Omar Salim, Paul R Lambden, Ian N Clarke
Publikováno v:
PLoS ONE, Vol 4, Iss 11, p e7723 (2009)
BACKGROUND:Chlamydia trachomatis is a major human pathogen with a unique obligate intracellular developmental cycle that takes place inside a modified cytoplasmic structure known as an inclusion. Following entry into a cell, the infectious elementary
Externí odkaz:
https://doaj.org/article/422e1b9aad384f6faf2e4c253adad536
Autor:
Sarah A. Pearson, David W. Cleary, Nicholas R. Thomson, David J. Lampe, Ian N. Clarke, Colette E. O'Neill, Jade Forster, Rachel J. Skilton
Publikováno v:
Wellcome Open Research, Vol 6 (2021)
Background: Chlamydia trachomatis is a prolific human pathogen that can cause serious long-term conditions if left untreated. Recent developments in Chlamydia genetics have opened the door to conducting targeted and random mutagenesis experiments to
Externí odkaz:
https://doaj.org/article/71bf456426164945a72f754b32424d84
Publikováno v:
Wellcome Open Research, Vol 6 (2021)
Background Genetic systems have been developed for Chlamydia but the extremely low transformation frequency remains a significant bottleneck. Our goal is to develop a self-replicating transposon delivery vector for C. trachomatis which can be expande
Externí odkaz:
https://doaj.org/article/02d4ed1c1f324244a0b5883d3455bfa8
Autor:
Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
Publikováno v:
mSphere, Vol 3, Iss 5 (2018)
ABSTRACT We demonstrate the genetic transformation of Chlamydia pneumoniae using a plasmid shuttle vector system which generates stable transformants. The equine C. pneumoniae N16 isolate harbors the 7.5-kb plasmid pCpnE1. We constructed the plasmid
Externí odkaz:
https://doaj.org/article/2d4f7be506794708bdf524dd71f61dc5
Autor:
Rachel J. Skilton, Yibing Wang, Colette O'Neill, Simone Filardo, Peter Marsh, Angèle Bénard, Nicholas R. Thomson, Kyle H. Ramsey, Ian N. Clarke
Publikováno v:
Wellcome Open Research, Vol 3 (2018)
Background: Research in chlamydial genetics is challenging because of its obligate intracellular developmental cycle. In vivo systems exist that allow studies of different aspects of basic biology of chlamydiae, the murine Chlamydia muridarum model i
Externí odkaz:
https://doaj.org/article/cad860f524264d25881cd4f138c5f176