Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Rachel J Skilton"'
Autor:
Sarah A. Pearson, David W. Cleary, Nicholas R. Thomson, David J. Lampe, Ian N. Clarke, Colette E. O'Neill, Jade Forster, Rachel J. Skilton
Publikováno v:
Wellcome Open Research, Vol 6 (2021)
Background: Chlamydia trachomatis is a prolific human pathogen that can cause serious long-term conditions if left untreated. Recent developments in Chlamydia genetics have opened the door to conducting targeted and random mutagenesis experiments to
Externí odkaz:
https://doaj.org/article/71bf456426164945a72f754b32424d84
Publikováno v:
Wellcome Open Research, Vol 6 (2021)
Background Genetic systems have been developed for Chlamydia but the extremely low transformation frequency remains a significant bottleneck. Our goal is to develop a self-replicating transposon delivery vector for C. trachomatis which can be expande
Externí odkaz:
https://doaj.org/article/02d4ed1c1f324244a0b5883d3455bfa8
Autor:
Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
Publikováno v:
mSphere, Vol 3, Iss 5 (2018)
ABSTRACT We demonstrate the genetic transformation of Chlamydia pneumoniae using a plasmid shuttle vector system which generates stable transformants. The equine C. pneumoniae N16 isolate harbors the 7.5-kb plasmid pCpnE1. We constructed the plasmid
Externí odkaz:
https://doaj.org/article/2d4f7be506794708bdf524dd71f61dc5
Autor:
Rachel J. Skilton, Yibing Wang, Colette O'Neill, Simone Filardo, Peter Marsh, Angèle Bénard, Nicholas R. Thomson, Kyle H. Ramsey, Ian N. Clarke
Publikováno v:
Wellcome Open Research, Vol 3 (2018)
Background: Research in chlamydial genetics is challenging because of its obligate intracellular developmental cycle. In vivo systems exist that allow studies of different aspects of basic biology of chlamydiae, the murine Chlamydia muridarum model i
Externí odkaz:
https://doaj.org/article/cad860f524264d25881cd4f138c5f176
Autor:
Colette E, O'Neill, Rachel J, Skilton, Jade, Forster, David W, Cleary, Sarah A, Pearson, David J, Lampe, Nicholas R, Thomson, Ian N, Clarke
Publikováno v:
Wellcome Open Research
Background: Chlamydia trachomatis is a prolific human pathogen that can cause serious long-term conditions if left untreated. Recent developments in Chlamydia genetics have opened the door to conducting targeted and random mutagenesis experiments to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::117167c17d6b510ad87d3e987ef472b3
https://pubmed.ncbi.nlm.nih.gov/35087955
https://pubmed.ncbi.nlm.nih.gov/35087955
Publikováno v:
Wellcome Open Research
BackgroundGenetic systems have been developed forChlamydiabut the extremely low transformation frequency remains a significant bottleneck. Our goal is to develop a self-replicating transposon delivery vector forC. trachomatiswhich can be expanded pri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::131af8adb778a7bdb97981ae54fd53cb
https://pubmed.ncbi.nlm.nih.gov/33997299
https://pubmed.ncbi.nlm.nih.gov/33997299
Autor:
Morgan R Herod, Omar Salim, Rachel J Skilton, Cynthia A Prince, Vernon K Ward, Paul R Lambden, Ian N Clarke
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e90679 (2014)
Investigations into human norovirus infection, replication and pathogenesis, as well as the development of potential antiviral agents, have been restricted by the lack of a cell culture system for human norovirus. To date, the optimal cell culture su
Externí odkaz:
https://doaj.org/article/2bb8d2b4de25413f84d7066b538b8b0e
Autor:
Yibing Wang, Simona Kahane, Lesley T Cutcliffe, Rachel J Skilton, Paul R Lambden, Kenneth Persson, Carina Bjartling, Ian N Clarke
Publikováno v:
PLoS ONE, Vol 8, Iss 3, p e59195 (2013)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (r
Externí odkaz:
https://doaj.org/article/3fbc15f3ffdf44f8ad7a56d3d7a12204
Autor:
Bryony Armson, Doriana Cellura, Nicholas Machin, Claire Bryer, David W. Cleary, Alex G. Richter, Veronica L. Fowler, Hana Sheldon, Deborah Porter, Zandra Deans, Tim Williams, Sarah Frampton, Nusreen Ahmad, Rachel J. Skilton, Nicholas Cortes, Konstantinos Boukas, Andrew D Beggs, Sue Hill, Jade Forster, Emma L. Wise, Joe Parker, Keith M. Godfrey, Gemma Snell, Angela Douglas, Andrew Bosworth, Rebecca Anderson, Nichola Graham, Simon Friar, Emma Garratt, Chris Mattocks, Charlotte Poxon, Emma L.A. Howson, Celina Whalley, Stephen P. Kidd, Tony Williams, Alice Goring, Alla Collins, Michael Kidd, Anetta Ptasinska, Seden Grippon
Introduction: Rapid, high throughput diagnostics are a valuable tool, allowing the detection of SARS-CoV-2 in populations, in order to identify and isolate people with asymptomatic and symptomatic infections. Reagent shortages and restricted access t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::77889cfe6c19368878129f7b1221bdf5
https://doi.org/10.1101/2020.12.15.20247031
https://doi.org/10.1101/2020.12.15.20247031
Autor:
Clare eLabiran, Ian N Clarke, Lesley T Cutcliffe, Yibing eWang, Rachel J Skilton, Kenneth ePersson, Carina eBjartling, Björn eHerrmann, Linus eChristerson, Peter eMarsh
Publikováno v:
Frontiers in Cellular and Infection Microbiology, Vol 2 (2012)
We aimed to evaluate the stability of the Chlamydia trachomatis MLVA-ompA and MST typing systems through multiple passages in tissue culture. Firstly, we analysed the stability of these markers through adaptation of C.trachomatis to tissue culture an
Externí odkaz:
https://doaj.org/article/fc70c4b532a74ad9ad39b37d4c1a2a65