Zobrazeno 1 - 10
of 299
pro vyhledávání: '"Rachel , Green"'
A small molecule that induces translational readthrough of CFTR nonsense mutations by eRF1 depletion
Autor:
Jyoti Sharma, Ming Du, Eric Wong, Venkateshwar Mutyam, Yao Li, Jianguo Chen, Jamie Wangen, Kari Thrasher, Lianwu Fu, Ning Peng, Liping Tang, Kaimao Liu, Bini Mathew, Robert J. Bostwick, Corinne E. Augelli-Szafran, Hermann Bihler, Feng Liang, Jerome Mahiou, Josef Saltz, Andras Rab, Jeong Hong, Eric J. Sorscher, Eric M. Mendenhall, Candice J. Coppola, Kim M. Keeling, Rachel Green, Martin Mense, Mark J. Suto, Steven M. Rowe, David M. Bedwell
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Premature termination codons can cause early translation termination and lead to disease. Here the authors perform a screen to identify compounds with readthrough activity and show that these reduce eRF1 levels to suppress premature termination assoc
Externí odkaz:
https://doaj.org/article/1010cb2b07ac42418e6fb9795f6fb27c
Distinct elongation stalls during translation are linked with distinct pathways for mRNA degradation
Autor:
Anthony J Veltri, Karole N D'Orazio, Laura N Lessen, Raphael Loll-Krippleber, Grant W Brown, Rachel Green
Publikováno v:
eLife, Vol 11 (2022)
Key protein adapters couple translation to mRNA decay on specific classes of problematic mRNAs in eukaryotes. Slow decoding on non-optimal codons leads to codon-optimality-mediated decay (COMD) and prolonged arrest at stall sites leads to no-go decay
Externí odkaz:
https://doaj.org/article/f6b1be037f56465d89e22d9c4d7f534c
Publikováno v:
PLoS Genetics, Vol 17, Iss 10, p e1009813 (2021)
Translation of messenger RNAs (mRNAs) with premature termination codons produces truncated proteins with potentially deleterious effects. This is prevented by nonsense-mediated mRNA decay (NMD) of these mRNAs. NMD is triggered by ribosomes terminatin
Externí odkaz:
https://doaj.org/article/fa5279b562774fcdb51142e48baba02b
Autor:
Rachel Green
Publikováno v:
Middle Eastern Literatures. :1-27
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01efb3818b463f49a8ed1229f3042cc7
https://doi.org/10.1080/1475262x.2023.2179388
https://doi.org/10.1080/1475262x.2023.2179388
Publikováno v:
eLife, Vol 9 (2020)
We used ribosome profiling to characterize the biological role of ribosome recycling factor (RRF) in Escherichia coli. As expected, RRF depletion leads to enrichment of post-termination 70S complexes in 3′-UTRs. We also observe that elongating ribo
Externí odkaz:
https://doaj.org/article/68d9d2665f7c444f851c86fd50165be0
Autor:
Niladri K Sinha, Alban Ordureau, Katharina Best, James A Saba, Boris Zinshteyn, Elayanambi Sundaramoorthy, Amit Fulzele, Danielle M Garshott, Timo Denk, Matthias Thoms, Joao A Paulo, J Wade Harper, Eric J Bennett, Roland Beckmann, Rachel Green
Publikováno v:
eLife, Vol 9 (2020)
Translation of aberrant mRNAs induces ribosomal collisions, thereby triggering pathways for mRNA and nascent peptide degradation and ribosomal rescue. Here we use sucrose gradient fractionation combined with quantitative proteomics to systematically
Externí odkaz:
https://doaj.org/article/900a514023304981b6374d2c536978ff
Autor:
Syed Usman Enam, Boris Zinshteyn, Daniel H Goldman, Madeline Cassani, Nathan M Livingston, Geraldine Seydoux, Rachel Green
Publikováno v:
eLife, Vol 9 (2020)
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The developmen
Externí odkaz:
https://doaj.org/article/4f34ce7e063048d9bde53f7974f89f11
Publikováno v:
eLife, Vol 9 (2020)
Shine-Dalgarno (SD) motifs are thought to play an important role in translational initiation in bacteria. Paradoxically, ribosome profiling studies in E. coli show no correlation between the strength of an mRNA’s SD motif and how efficiently it is
Externí odkaz:
https://doaj.org/article/8627f1244caa4abab56f0778a0beb878
Autor:
Jamie R Wangen, Rachel Green
Publikováno v:
eLife, Vol 9 (2020)
Stop codon readthrough (SCR) occurs when the ribosome miscodes at a stop codon. Such readthrough events can be therapeutically desirable when a premature termination codon (PTC) is found in a critical gene. To study SCR in vivo in a genome-wide manne
Externí odkaz:
https://doaj.org/article/4f26ec2f78ee4199bcedebbff5471f92