Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain α-neurotoxins from snakes

Autor: Line Ledsgaard, Jack Wade, Timothy P. Jenkins, Kim Boddum, Irina Oganesyan, Julian A. Harrison, Pedro Villar, Rachael A. Leah, Renato Zenobi, Sanne Schoffelen, Bjørn Voldborg, Anne Ljungars, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Andreas H. Laustsen, Aneesh Karatt-Vellatt

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)

The treatment of snakebite envenoming is currently suboptimal. Existing antivenoms often lack efficacy and may cause adverse reactions. Here, the authors derive, develop, and demonstrate the utility of toxin-specific broadly-neutralizing human monocl

Externí odkaz: https://doaj.org/article/e25513bb2bca42949088d83d90b24bca

In vivo neutralization of dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG antibodies

Autor: Andreas H. Laustsen, Aneesh Karatt-Vellatt, Edward W. Masters, Ana Silvia Arias, Urska Pus, Cecilie Knudsen, Saioa Oscoz, Peter Slavny, Daniel T. Griffiths, Alice M. Luther, Rachael A. Leah, Majken Lindholm, Bruno Lomonte, José María Gutiérrez, John McCafferty

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)

Current anti-venoms against black mamba (Dendroaspis polylepis) bites are animal-derived and associated with several limitations. Here, Laustsen and colleagues develop an experimental recombinant anti-venom based on oligoclonal human IgG antibodies a

Externí odkaz: https://doaj.org/article/ddd6fff5db93422e9ee2acd780659e94

Discovery of a broadly-neutralizing human antibody that can rescue mice challenged with neurotoxin-rich snake venoms

Autor: Line Ledsgaard, Jack Wade, Kim Boddum, Irina Oganesyan, Julian Harrison, Timothy P. Jenkins, Pedro Villar, Rachael A. Leah, Renato Zenobi, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Andreas H. Laustsen, Aneesh Karatt-Vellatt

Snakebite envenoming continues to claim many lives across the globe, necessitating the development of improved therapies. To this end, human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6a1377b44e808d5ea27a3dc220a46b24
https://doi.org/10.1101/2022.06.17.496531

A comprehensive search of functional sequence space using large mammalian display libraries created by gene editing

Autor: Edward W. Masters, Sophie Mayle, Daniel T. Griffiths, Maheen Sattar, John McCafferty, Rachael A. Leah, Yanchao Huang, Kothai Parthiban, Rajika L. Perera, Michael R. Dyson, Sachin Surade

Publikováno v: mAbs

The construction of large libraries in mammalian cells allows the direct screening of millions of molecular variants for binding properties in a cell type relevant for screening or production. We have created mammalian cell libraries of up to 10 mill

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e85ac50a098360a84026aead007c972
http://europepmc.org/articles/PMC6601556

In vivo neutralization of dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG antibodies

Autor: Daniel T. Griffiths, Edward W. Masters, Bruno Lomonte, Majken Lindholm, Saioa Oscoz, Rachael A. Leah, Aneesh Karatt-Vellatt, José María Gutiérrez, Ana Silvia Arias, Urska Pus, Alice M. Luther, John McCafferty, Peter Slavny, Andreas Hougaard Laustsen, Cecilie Knudsen

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Laustsen, A H, Karatt-Vellatt, A, Masters, E W, Arias, A S, Pus, U, Knudsen, C, Oscoz, S, Slavny, P, Griffiths, D T, Luther, A M, Leah, R A, Lindholm, M, Lomonte, B, Gutiérrez, J M & McCafferty, J 2018, ' In vivo neutralization of dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG antibodies ', Nature Communications, vol. 9, no. 1, 3928 . https://doi.org/10.1038/s41467-018-06086-4

The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and α-neurotoxins associated with high fatality in untreated victims. Cur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c7988b2f1ebc606e87941263119af71
http://link.springer.com/article/10.1038/s41467-018-06086-4