Výsledky vyhledávání - "RT, Reverse Transcriptase"

In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the Raindance ddPCR platform

Autor: Samuel Long

Publikováno v: Methods (San Diego, Calif.)

Sensitive PCR detection of viral nucleic acids plays a critical role in infectious disease research, diagnosis and monitoring. In the context of SARS-CoV-2 detection, recent reports indicate that digital PCR-based tests are significantly more sensiti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40ffd41efd3a3c73b76e2e0fb9f5b477
https://doi.org/10.1016/j.ymeth.2021.04.008

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Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis

Autor: Yoshinori Okamoto, Hideto Jinno, Yoko Mori, Akira Aoki

Publikováno v: Journal of Infection and Chemotherapy

Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus causing coronavirus disease 2019 (COVID-19), has been expanding globally since late 2019. SARS-CoV-2, an RNA virus, has a genome sequence that can easily u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e2fd34c61bf93f9750f7af8e24fc1c9
https://doi.org/10.1016/j.jiac.2021.06.007

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Eclampsia and posterior reversible encephalopathy syndrome in a parturient complicated by SARS COVID-19 pneumonia

Autor: Vijay Shankar, Jai George, Purnima Dhar, Anil Sharma, Anupam Raj

Publikováno v: Brazilian Journal of Anesthesiology (Elsevier)
Brazilian Journal of Anesthesiology, Vol 71, Iss 5, Pp 576-578 (2021)

A primigravida at 32 weeks of gestation presented to us with eclampsia and Posterior Reversible Encephalopathy Syndrome (PRES) along with SARS COVID-19 pneumonia. Immediate termination of pregnancy was done under general anesthesia and patient was el

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::628a18cb9cd18a00b0b1042979262d18
https://doi.org/10.1016/j.bjane.2021.06.008

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Development of non-nucleoside reverse transcriptase inhibitors (NNRTIs): our past twenty years

Autor: Christophe Pannecouque, Erik De Clercq, Chunlin Zhuang, Fen-Er Chen

Publikováno v: Acta Pharmaceutica Sinica. B
Acta Pharmaceutica Sinica B, Vol 10, Iss 6, Pp 961-978 (2020)

Human immunodeficiency virus (HIV) is the primary infectious agent of acquired immunodeficiency syndrome (AIDS), and non-nucleoside reverse transcriptase inhibitors (NNRTIs) are the cornerstone of HIV treatment. In the last 20 years, our medicinal ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18dcf4613668fe3b2dc9c50a8c30573f
https://doi.org/10.1016/j.apsb.2019.11.010

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Improving the positional adaptability: structure-based design of biphenyl-substituted diaryltriazines as novel non-nucleoside HIV-1 reverse transcriptase inhibitors

Autor: Chunlin Zhuang, Fen-Er Chen, Ge Meng, KaiJun Jin, Erik De Clercq, Christophe Pannecouque, Minjie Liu

Publikováno v: Acta Pharmaceutica Sinica B, Vol 10, Iss 2, Pp 344-357 (2020)
Acta Pharmaceutica Sinica. B

In order to improve the positional adaptability of our previously reported naphthyl diaryltriazines (NP-DATAs), synthesis of a series of novel biphenyl-substituted diaryltriazines (BP-DATAs) with a flexible side chain attached at the C-6 position is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::669bcf19e6301aee51c948cba93b6a27
http://www.sciencedirect.com/science/article/pii/S2211383519301832

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Ty1 integrase is composed of an active N-terminal domain and a large disordered C-terminal module dispensable for its activity $in\ vitro$

Autor: Nguyen, Phong Quoc, Conesa, Christine, Rabut, Elise, Bragagnolo, Gabriel, Gouzerh, Célia, Fernández-Tornero, Carlos, Lesage, Pascale, Reguera, Juan, Acker, Joël

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, 2021, 297 (4), pp.101093. ⟨10.1016/j.jbc.2021.101093⟩
The Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2021, 297 (4), pp.101093. ⟨10.1016/j.jbc.2021.101093⟩

International audience; Long-terminal repeat (LTR) retrotransposons are genetic elements that, like retroviruses, replicate by reverse transcription of an RNA intermediate into a complementary DNA (cDNA) that is next integrated into the host genome b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::752cd905cee6d9885a55819773773c7c
https://amu.hal.science/hal-03402697/file/pho1.pdf

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Optimization of reaction condition of recombinase polymerase amplification to detect SARS-CoV-2 DNA and RNA using a statistical method

Autor: Shinsuke Fujiwara, Kevin Maafu Juma, Kiyoshi Yasukawa, Shihomi Akagi, Manish Biyani, Yukiko Nakura, Itaru Yanagihara, Kenji Ito, Emi Arikawa, Masaya Yamagata, Teisuke Takita, Kenji Kojima

Publikováno v: Biochemical and Biophysical Research Communications

Recombinase polymerase amplification (RPA) is an isothermal reaction that amplifies a target DNA sequence with a recombinase, a single-stranded DNA-binding protein (SSB), and a strand-displacing DNA polymerase. In this study, we optimized the reactio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fde093c5c425fce3a57df7581a241da
https://pubmed.ncbi.nlm.nih.gov/34166918

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Structural basis for template switching by a group II intron-encoded non-LTR-retroelement reverse transcriptase

Autor: Alan M. Lambowitz, Rick Russell, Jennifer L. Stamos, Jun Yao, Alfred M. Lentzsch

Publikováno v: The Journal of Biological Chemistry

Reverse transcriptases (RTs) can template switch during cDNA synthesis, enabling them to join discontinuous nucleic acid sequences. Template switching plays crucial roles in retroviral replication and recombination, is used for adapter addition in RN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b458fcf4b0b09c19a9203a6de206a868
https://doi.org/10.1101/2021.05.13.443781

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AncPhore: A versatile tool for anchor pharmacophore steered drug discovery with applications in discovery of new inhibitors targeting metallo

Autor: Qingqing, Dai, Yuhang, Yan, Xiangli, Ning, Gen, Li, Junlin, Yu, Ji, Deng, Lingling, Yang, Guo-Bo, Li

Publikováno v: Acta Pharmaceutica Sinica. B

We herein describe AncPhore, a versatile tool for drug discovery, which is characterized by pharmacophore feature analysis and anchor pharmacophore (i.e., most important pharmacophore features) steered molecular fitting and virtual screening. Compara

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https://pubmed.ncbi.nlm.nih.gov/34386329

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Identification of the ternary complex of ribonuclease HI:RNA/DNA hybrid:metal ions by ESI mass spectrometry

Autor: Shigenori Kanaya, Nobuaki Okumura, Tomoshige Ando, Toshifumi Takao, Nujarin Jongruja, Kosuke Morikawa

Publikováno v: The Journal of Biological Chemistry

Ribonuclease HI, an endoribonuclease, catalyzes the hydrolysis of the RNA strand of an RNA/DNA hybrid and requires divalent metal ions for its enzymatic activity. However, the mechanistic details of the activity of ribonuclease HI and its interaction

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f139293458754ee8fae71969bc03b97
https://pubmed.ncbi.nlm.nih.gov/33639158

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