Zobrazeno 1 - 10
of 76
pro vyhledávání: '"ROBERT B. TRIMBLE"'
Publikováno v:
Journal of Biological Chemistry. 279:35644-35655
The N-linked galactomannans of Schizosaccharomyces pombe have pyruvylated Galbeta1,3-(PvGal) caps on a portion of the Galalpha1,2-residues in their outer chains (Gemmill, T. R., and Trimble, R. B. (1998) Glycobiology 8, 1087-1095). PvGal biosynthesis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7b955be40897fb090eae95fe9540954
https://doi.org/10.1074/jbc.m403574200
https://doi.org/10.1074/jbc.m403574200
Autor:
Charles R. Hauer, Trent R. Gemmill, Catherine Lubowski, Robert B. Trimble, S. Anand Kumar, Lynn McNaughton, Robert F. Stack
Publikováno v:
Glycobiology. 14:265-274
Recombinant human bile salt-stimulated lipase (hBSSL) was expressed in and secreted by Pichia pastoris, an organism exploited for the large-scale production of recombinant (glyco)proteins by bioprocessing technology. The 76.3-kDa glycoprotein was ass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b984a10a635d14d2e3e96176c3304b20
https://doi.org/10.1093/glycob/cwh036
https://doi.org/10.1093/glycob/cwh036
Autor:
Robert B. Trimble, John F. Cipollo
Publikováno v:
Glycobiology. 12:30G-33G
The Saccharomyces cerevisiae alg12delta mutant accumulates oligosaccharide lipid with a Man(7)GlcNAc(2) oligosaccharide. To determine the N-glycan structures present on S. cerevisiae glycoproteins in the alg12delta strain, we made attempts to purify
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::69af02a55d051361502f0ffc75ba63c4
https://doi.org/10.1093/glycob/cwf083
https://doi.org/10.1093/glycob/cwf083
Autor:
Robert B. Trimble, John F. Cipollo
Publikováno v:
Glycobiology. 12:749-762
N-glycosylation in nearly all eukaryotes proceeds in the endoplasmic reticulum (ER) by transfer of the precursor Glc(3)Man(9)GlcNAc(2) from dolichyl pyrophosphate (PP-Dol) to consensus Asn residues in nascent proteins. The Saccharomyces cerevisiae al
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c38feeca0d4317f850dcfbdfddf0ff3
https://doi.org/10.1093/glycob/cwf082
https://doi.org/10.1093/glycob/cwf082
Publikováno v:
Journal of Biological Chemistry. 276:21828-21840
The initial steps in N-linked glycosylation involve the synthesis of a lipid-linked core oligosaccharide followed by the transfer of the core glycan to nascent polypeptides in the endoplasmic reticulum (ER). Here, we describe alg11, a new yeast glyco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7006692af6f2e16174fb184ac8eefbf7
https://doi.org/10.1074/jbc.m010896200
https://doi.org/10.1074/jbc.m010896200
Publikováno v:
Analytical Biochemistry. 278:111-122
A major difficulty with isolating enzymatically or chemically released oligosaccharides from large-scale glycoprotein deglycosylation reactions is the time-consuming chromatography, desalting, and concentration steps required to prepare a glycan frac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::149f2c046dcf7d3637839d766e6bfaf1
https://doi.org/10.1006/abio.1999.4433
https://doi.org/10.1006/abio.1999.4433
Autor:
Robert B. Trimble, Trent R. Gemmill
Publikováno v:
Glycobiology. 9:507-515
Schizosaccharomyces pombe whole-cell glycoproteins, previously depleted of N-linked glycans by sequential treatment with endo-ss-N-acetylglucosaminidase H and peptide-N4-asparagine amidohydrolase F, were ss-eliminated with 0.1 M NaOH/1 M NaBH4 to rel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19aa4faa4c71c4a3b8af930e70ed16b4
https://doi.org/10.1093/glycob/9.5.507
https://doi.org/10.1093/glycob/9.5.507
Autor:
Robert B. Trimble, Trent R. Gemmill
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1426:227-237
Yeast and most higher eukaryotes utilize an evolutionarily conserved N-linked oligosaccharide biosynthetic pathway that involves the formation of a Glc3Man9GlcNAc2-PP-dolichol lipid-linked precursor, the glycan portion of which is co-translationally
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a48eaa5a3d1916bc6ae6df4bdf8f444b
https://doi.org/10.1016/s0304-4165(98)00126-3
https://doi.org/10.1016/s0304-4165(98)00126-3
Autor:
Robert B. Trimble, Trent R. Gemmill
Publikováno v:
Journal of Biological Chemistry. 271:25945-25949
The large N-linked oligosaccharides released by endo-beta-N-acetylglucosaminidase H from Schizosaccharomyces pombe glycoproteins were analyzed for the presence of noncarbohydrate functional groups. No phosphate, sulfate, or acetate could be detected;
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e85da19158fed92db0f0fcf6ccd4b67d
https://doi.org/10.1074/jbc.271.42.25945
https://doi.org/10.1074/jbc.271.42.25945
Publikováno v:
Glycobiology. 5:671-681
In the yeast Golgi compartments, at least five, and potentially several additional mannosyltransferases are involved in elongating to 'mannan' the core Man8GlcNAc2 oligosaccharide trimmed from Glc3Man9GlcNAc2 in the endoplasmic reticulum. Structural
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12331f88235bddbe822e0f7c9a521f96
https://doi.org/10.1093/glycob/5.7.671
https://doi.org/10.1093/glycob/5.7.671