Real-Time Single-Molecule Studies of RNA Polymerase–Promoter Open Complex Formation Reveal Substantial Heterogeneity Along the Promoter-Opening Pathway

Autor: Emil Aalto-Setälä, Georgiy A. Belogurov, Jacob Bakermans, David L.V. Bauer, Anssi M. Malinen, Olena Parilova, David Dulin, Martin Blessing, Achillefs N. Kapanidis

Publikováno v: Journal of Molecular Biology
Malinen, A M, Bakermans, J, Aalto-Setälä, E, Blessing, M, Bauer, D L V, Parilova, O, Belogurov, G A, Dulin, D & Kapanidis, A N 2022, ' Real-Time Single-Molecule Studies of RNA Polymerase–Promoter Open Complex Formation Reveal Substantial Heterogeneity Along the Promoter-Opening Pathway ', Journal of Molecular Biology, vol. 434, no. 2, 167383, pp. 1-18 . https://doi.org/10.1016/j.jmb.2021.167383
Journal of Molecular Biology, 434(2):167383, 1-18. Academic Press Inc.

Graphical abstract
Highlights • The formation of single RNA polymerase–promoter open complexes probed in real-time. • Substantial heterogeneity was found along the promoter-opening pathway. • Branched steps include template strand loadin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d45174421ad539b45f3f4ba85ff46ca
https://research.vu.nl/en/publications/a6f8678f-2f44-48c2-a1c6-551c25cefd41

On a stake-out: Mycobacterial small RNA identification and regulation

Autor: Sagarika Taneja, Tanmay Dutta

Publikováno v: Non-coding RNA Research
Non-coding RNA Research, Vol 4, Iss 3, Pp 86-95 (2019)

Persistence of mycobacteria in the hostile environment of human macrophage is pivotal for its successful pathogenesis. Rapid adaptation to diverse stresses is the key aspect for their survival in the host cells. A range of heterogeneous mechanisms op

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::671f3ccacad6b49decbf4a75b5c66b8a
https://doi.org/10.1016/j.ncrna.2019.05.001

Revisiting T7 RNA polymerase transcription in vitro with the Broccoli RNA aptamer as a simplified real-time fluorescent reporter

Autor: Keith T. Gagnon, Helen I Janis, Shaoni Mukhopadhyay, Zachary J. Kartje

Publikováno v: The Journal of Biological Chemistry

Methods for rapid and high-throughput screening of transcription in vitro to examine reaction conditions, enzyme mutants, promoter variants, and small molecule modulators can be extremely valuable tools. However, these techniques may be difficult to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bfa2c952cb100261ef8c4fb81942292
http://europepmc.org/articles/PMC7948468

Structural basis of RNA polymerase recycling by the Swi2/Snf2 family of ATPase RapA in Escherichia coli

Autor: Katsuhiko S. Murakami, M. Zuhaib Qayyum, Vadim Molodtsov, Andrew Renda

Publikováno v: The Journal of Biological Chemistry

After transcription termination, cellular RNA polymerases (RNAPs) are occasionally trapped on DNA, impounded in an undefined Post-Termination Complex (PTC), limiting the free RNAP pool and subsequently leading to inefficient transcription. In Escheri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09b6bc69d84065caa0afc4b7aaefcfd5
https://doi.org/10.1016/j.jbc.2021.101404

Region 4 of the RNA polymerase σ subunit counteracts pausing during initial transcription

Autor: Zakia Morichaud, Konstantin Brodolin

Publikováno v: The Journal of Biological Chemistry

All cellular genetic information is transcribed into RNA by multisubunit RNA polymerases (RNAPs). The basal transcription initiation factors of cellular RNAPs stimulate the initial RNA synthesis via poorly understood mechanisms. Here, we explored the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c41d827c88aca68e69d9fe02f0322487
https://doi.org/10.1074/jbc.ra120.016299