Zobrazeno 1 - 10
of 7 583
pro vyhledávání: '"RNA EXTRACTION"'
Publikováno v:
Applications in Plant Sciences, Vol 12, Iss 6, Pp n/a-n/a (2024)
Abstract Premise The extraction of high‐quality RNA is the critical first step for the analysis of gene expression and gene space. This remains particularly challenging in plants, and especially in ferns, where the disruption of the cell wall and s
Externí odkaz:
https://doaj.org/article/0815bfdeedae41cfaeb3c0af6f3341d9
Autor:
Leobardo Daniel Gonzalez-Zuñiga, Libia Zulema Rodriguez-Anaya, Jose Reyes Gonzalez-Galaviz, Abraham Cruz-Mendívil, Fernando Lares-Villa, Luis Fernando Lares-Jiménez
Publikováno v:
Parasitologia, Vol 4, Iss 2, Pp 199-208 (2024)
Balamuthia mandrillaris is a free-living amoeba (FLA) that causes granulomatous amebic encephalitis (GAE) and skin lesions. Transcriptomic analysis is a powerful tool used to study B. mandrillaris pathogenic infections. However, preliminary tests of
Externí odkaz:
https://doaj.org/article/2d611d2a70e944ab9ecdd52622a87c18
Autor:
Tibor Kiss, Zoltán Karácsony, Adrienn Gomba-Tóth, Kriszta Lilla Szabadi, Zsolt Spitzmüller, Júlia Hegyi-Kaló, Thomas Cels, Margot Otto, Richárd Golen, Ádám István Hegyi, József Geml, Kálmán Zoltán Váczy
Publikováno v:
Plant Methods, Vol 20, Iss 1, Pp 1-7 (2024)
Abstract Background High-quality RNA extraction from woody plants is difficult because of the presence of polysaccharides and polyphenolics that bind or co-precipitate with the RNA. The CTAB (cetyl trimethylammonium bromide) based method is widely us
Externí odkaz:
https://doaj.org/article/70821cd2f3d04228a56c7a836072206d
Publikováno v:
PeerJ, Vol 12, p e18072 (2024)
Background Extracting high-quality total RNA is pivotal for advanced RNA molecular studies, such as Next-generation sequencing and expression microarrays where RNA is hybridized. Despite the development of numerous extraction methods in recent decade
Externí odkaz:
https://doaj.org/article/bf85f80e9aeb45c8b5c69cb32d357871
Autor:
Jason D. Limberis, John Z. Metcalfe
Publikováno v:
HardwareX, Vol 19, Iss , Pp e00576- (2024)
turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of
Externí odkaz:
https://doaj.org/article/8eb386147ac746f78073ea43b29db2e0
Autor:
Elody Orcel, Hayat Hage, May Taha, Noémie Boucher, Emilie Chautard, Virginie Courtois, Adrien Saliou
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-16 (2024)
Abstract Background Blood transcriptomic analysis is widely used to provide a detailed picture of a physiological state with potential outcomes for applications in diagnostics and monitoring of the immune response to vaccines. However, multi-species
Externí odkaz:
https://doaj.org/article/d5c4a753fb8249b19f6ee4c60a79d693
Autor:
Ahadi Damar Prasetya, Muflikhah Muflikhah, Wildan Zakiah Lubis, Andon Insani, Grace Tjungirai Sulungbudi, Mujamilah Mujamilah, Uus Saepulloh
Publikováno v:
Indonesian Journal of Chemistry, Vol 24, Iss 1, Pp 81-93 (2024)
Since the end of 2019, COVID-19 pandemic caused by the novel SARS-CoV-2 has become a serious problem for the world. Accurate and rapid techniques in testing and tracing are needed to control the virus spreading. Molecular diagnostics through gene amp
Externí odkaz:
https://doaj.org/article/6826c717c8c9462eaeac962a5e2e6ed9
Publikováno v:
Frontiers in Molecular Biosciences, Vol 11 (2024)
Introduction: Human saliva was used to develop non-invasive liquid biopsy biomarkers to establish saliva as an alternate to blood and plasma in translational research. The present study focused on understanding the impact of sample storage conditions
Externí odkaz:
https://doaj.org/article/aa682b916c23410ba02577c76c20072c
Autor:
Simran Maggo, Liam Y. North, Aime Ozuna, Dejerianne Ostrow, Yander R. Grajeda, Hesamedin Hakimjavadi, Jennifer A. Cotter, Alexander R. Judkins, Pat Levitt, Xiaowu Gai
Publikováno v:
Frontiers in Pediatrics, Vol 12 (2024)
The mitochondrion is a multifunctional organelle that modulates multiple systems critical for homeostasis during pathophysiological stress. Variation in mitochondrial DNA (mtDNA) copy number (mtDNAcn), a key mitochondrial change associated with chron
Externí odkaz:
https://doaj.org/article/c4a5d9846d754dc9bc7df7a2c19feabc
Publikováno v:
Viruses, Vol 16, Iss 9, p 1442 (2024)
Since early 2024, a multistate outbreak of highly pathogenic avian influenza H5N1 has been affecting dairy cattle in the USA. The influenza viral RNA concentrations in milk make it an ideal matrix for surveillance purposes. However, viral RNA detecti
Externí odkaz:
https://doaj.org/article/8129f502ebfe417487c56b9ee9d79372