Ribozyme-activated mRNA trans-ligation enables large gene delivery to treat muscular dystrophies.

Autor: Lindley SR; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA., Subbaiah KCV; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA., Priyanka F; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA., Poosala P; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA., Ma Y; CANbridge Pharmaceuticals, Burlington, MA 01803, USA., Jalinous L; CANbridge Pharmaceuticals, Burlington, MA 01803, USA., West JA; CANbridge Pharmaceuticals, Burlington, MA 01803, USA., Richardson WA; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA., Thomas TN; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA., Anderson DM; Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.; Center for RNA Biology: From Genome to Therapeutics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2024 Nov 15; Vol. 386 (6723), pp. 762-767. Date of Electronic Publication: 2024 Nov 14.

Elucidating Evolutionary Mechanisms and Variants of the Hammerhead Ribozyme Using In Vitro Selection.

Autor: Brill J; Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada., Nurmi C; Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada., Li Y; Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.

Publikováno v: Chembiochem : a European journal of chemical biology [Chembiochem] 2024 Nov 04; Vol. 25 (21), pp. e202400432. Date of Electronic Publication: 2024 Oct 04.

A novel viral RNA detection method based on the combined use of trans-acting ribozymes and HCR-FRET analyses.

Autor: Ferreira da Silva L; Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil., Valle Garay A; Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., Queiroz PF; Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil., Garcia de Resende S; Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil., Gomide M; Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil., Moreira de Oliveira IC; Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., Souza Bernasol A; Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., Arce A; Institute for biological and medical engineering, Pontificia Universidad Católica de Chile, Santiago de Chile, Chile., Canet Santos L; Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., Torres F; Laboratory of Molecular Biology, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., Silva-Pereira I; Laboratory of Molecular Biology of Pathogenic Fungi, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., de Freitas SM; Laboratory of Molecular Biophysics, Department of Cell Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, Federal District, Brazil., Marques Coelho C; Laboratory of Synthetic Biology, Department of Genetics and Morphology, Institute of Biological Science, University of Brasília (UnB), Brasília, Federal District, Brazil.

Publikováno v: PloS one [PLoS One] 2024 Sep 26; Vol. 19 (9), pp. e0310171. Date of Electronic Publication: 2024 Sep 26 (Print Publication: 2024).

Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole to triphosphate activation.

Autor: DasGupta S; Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114.; HHMI, Massachusetts General Hospital, Boston, MA 02114.; Department of Genetics, Harvard Medical School, Boston, MA 02115., Weiss Z; Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114.; HHMI, Massachusetts General Hospital, Boston, MA 02114.; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138., Nisler C; HHMI, The University of Chicago, Chicago, IL 60637.; Department of Chemistry, The University of Chicago, Chicago, IL 60637., Szostak JW; Department of Molecular Biology, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114.; HHMI, Massachusetts General Hospital, Boston, MA 02114.; Department of Genetics, Harvard Medical School, Boston, MA 02115.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Sep 17; Vol. 121 (38), pp. e2407325121. Date of Electronic Publication: 2024 Sep 13.

Prebiotic chiral transfer from self-aminoacylating ribozymes may favor either handedness.

Autor: Kenchel J; Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA, USA.; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA.; Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA., Vázquez-Salazar A; Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA., Wells R; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA., Brunton K; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA., Janzen E; Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA, USA.; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA., Schultz KM; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA., Liu Z; Department of Earth Sciences, University of Cambridge, Cambridge, USA.; MRC - Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH, United Kingdom., Li W; Bren School of Environmental Management, University of California, Santa Barbara, CA, USA., Parker ET; Solar System Exploration Division, NASA Goddard Space Flight Center, Greenbelt, MD, USA., Dworkin JP; Solar System Exploration Division, NASA Goddard Space Flight Center, Greenbelt, MD, USA., Chen IA; Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA, USA. ireneachen@ucla.edu.; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA. ireneachen@ucla.edu.; Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA. ireneachen@ucla.edu.; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA. ireneachen@ucla.edu.

Publikováno v: Nature communications [Nat Commun] 2024 Sep 12; Vol. 15 (1), pp. 7980. Date of Electronic Publication: 2024 Sep 12.