Zobrazeno 1 - 10
of 33
pro vyhledávání: '"RK2 plasmid"'
Publikováno v:
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
The activity of type II toxin-antitoxin systems (TA), which are responsible for many important features of bacterial cells, is based on the differences between toxin and antitoxin stabilities. The antitoxin lability results from bacterial protease ac
Autor:
Monika Witosinska, Pawel Schweiger, Katarzyna Bury, Urs Jenal, Katarzyna Wegrzyn, Igor Konieczny
Publikováno v:
Microbiology
Undisturbed plasmid dynamics is required for the stable maintenance of plasmid DNA in bacterial cells. In this work, we analysed subcellular localization, DNA synthesis and nucleoprotein complex formation of plasmid RK2 during the cell cycle of Caulo
Publikováno v:
Plasmid. 64:119-134
The broad-host-range plasmid RK2 has been a model for studying DNA metabolism in bacteria for many years. It is used as a vector allowing genetic manipulations in numerous bacterial species. The RK2 genome encodes several genes providing the plasmid
Publikováno v:
Molecular Microbiology. 67:935-946
The highly conserved ParA family of partitioning systems is responsible for positioning DNA and protein complexes in bacteria. In Escherichia coli, plasmids that rely upon these systems are positioned at mid-cell and are repositioned at the quarter-c
Publikováno v:
Journal of Bacteriology. 187:3538-3547
Multicopy plasmids in Escherichia coli are not randomly distributed throughout the cell but exist as defined clusters that are localized at the mid-cell, or at the 1/4 and 3/4 cell length positions. To explore the factors that contribute to plasmid c
Autor:
Magdalena M. Felczak, Jon M. Kaguni
Publikováno v:
Journal of Biological Chemistry. 279:51156-51162
Escherichia coli DnaA protein initiates DNA replication from the chromosomal origin, oriC, and regulates the frequency of this process. Structure-function studies indicate that the replication initiator comprises four domains. Based on the structural
Autor:
Lyn Sue Kahng, Lucy Shapiro
Publikováno v:
Journal of Bacteriology. 185:3384-3391
The origins of replication of many different bacteria have been shown to reside at specific subcellular locations, but the mechanisms underlying their positioning and segregation are still being elucidated. In particular, little is known about the re
Autor:
Aresa Toukdarian, Donald R. Helinski
Publikováno v:
Gene. 223:205-211
Copy-number regulation of the broad-host-range plasmid RK2 is dependent on the plasmid-encoded initiator protein, TrfA, and the RK2 origin of replication. The handcuffing model for copy-number control proposes that TrfA-bound oris reversibly couple t
Publikováno v:
Journal of Bacteriology. 177:2789-2797
The par region of the broad-host-range, IncP alpha plasmid RK2 has been implicated as a stability determinant by its ability to enhance the maintenance of mini-RK2 plasmids or heterologous replicons in a growing population of host cells. The region c
Publikováno v:
Gene. 146:39-45
Cosmid pOJ436, containing large inserts of Saccharopolyspora spinosa (Ss) DNA, was transferred by conjugation from Escherichia coli to Ss an integrated into the chromosome, apparently by homologous recombination, at high frequencies (10(-5) to 10(-4)