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Akademický článek

Higher resolution in cryo-EM by the combination of macromolecular prior knowledge and image-processing tools

Autor: Erney Ramírez-Aportela, Jose M. Carazo, Carlos Oscar S. Sorzano

Publikováno v: IUCrJ, Vol 9, Iss 5, Pp 632-638 (2022)

Single-particle cryo-electron microscopy has become a powerful technique for the 3D structure determination of biological molecules. The last decade has seen an astonishing development of both hardware and software, and an exponential growth of new s

Externí odkaz: https://doaj.org/article/756bd17708674f538cf5c2382946dcdf

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Akademický článek

Performance and Quality Comparison of Movie Alignment Software for Cryogenic Electron Microscopy

Autor: David Střelák, Daniel Marchán, José María Carazo, Carlos O. S. Sorzano

Publikováno v: Micromachines, Vol 14, Iss 10, p 1835 (2023)

Cryogenic electron microscopy (Cryo-EM) has been established as one of the key players in structural biology. It can reconstruct a 3D model of a sample at a near-atomic resolution. With the increasing number of facilities, faster microscopes, and new

Externí odkaz: https://doaj.org/article/1942109439cd4492a1ee496917d9825a

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Akademický článek

High-resolution cryo-EM using beam-image shift at 200 keV

Autor: Jennifer N. Cash, Sarah Kearns, Yilai Li, Michael A. Cianfrocco

Publikováno v: IUCrJ, Vol 7, Iss 6, Pp 1179-1187 (2020)

Recent advances in single-particle cryo-electron microscopy (cryo-EM) data collection utilize beam-image shift to improve throughput. Despite implementation on 300 keV cryo-EM instruments, it remains unknown how well beam-image-shift data collection

Externí odkaz: https://doaj.org/article/ef86235a6b6c4fe48af265981d35ecf8

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Akademický článek

Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1

Autor: Jasenko Zivanov, Takanori Nakane, Sjors H. W. Scheres

Publikováno v: IUCrJ, Vol 7, Iss 2, Pp 253-267 (2020)

Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminar

Externí odkaz: https://doaj.org/article/70365abd15de459989db61c9573348cf

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Akademický článek

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Akademický článek

Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule

Autor: Samuel E Lacey, Shaoda He, Sjors HW Scheres, Andrew P Carter

Publikováno v: eLife, Vol 8 (2019)

Dyneins are motor proteins responsible for transport in the cytoplasm and the beating of axonemes in cilia and flagella. They bind and release microtubules via a compact microtubule-binding domain (MTBD) at the end of a coiled-coil stalk. We address

Externí odkaz: https://doaj.org/article/cdcefc48749b4cbab19416039d806876

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Akademický článek

Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases

Autor: Wenjuan Zhang, Benjamin Falcon, Alexey G Murzin, Juan Fan, R Anthony Crowther, Michel Goedert, Sjors HW Scheres

Publikováno v: eLife, Vol 8 (2019)

Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases. Tau filaments adopt different conformations in Alzheimer’s and Pick’s diseases. Here, we used cryo- and immuno- electron m

Externí odkaz: https://doaj.org/article/ee4f6398c129456c86cb564b7b6c494a

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