Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro

Autor: SANTOS, Letícia Martins, CARDOSO, Patricia e Silva, DINIZ, Elisa Abreu, RAHHAL, Juliana Garuba, SIPERT, Carla Renata

Publikováno v: Journal of Applied Oral Science, Volume: 31, Article number: e20230020, Published: 24 JUL 2023

Fetal bovine serum (FBS) is the most used supplement in culture media; however, it may interfere with in vitro assays via effects on cell proliferation and cytokine production. The ideal FBS concentration for assays using apical papilla cells (APCs)

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Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro.

Autor: Santos LM; Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brasil., Cardoso PES; Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brasil., Diniz EA; Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brasil., Rahhal JG; Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brasil., Sipert CR; Universidade de São Paulo, Departamento de Dentística, Faculdade de Odontologia, São Paulo, SP, Brasil.

Publikováno v: Journal of applied oral science : revista FOB [J Appl Oral Sci] 2023 Jul 24; Vol. 31, pp. e20230020. Date of Electronic Publication: 2023 Jul 24 (Print Publication: 2023).

Response of periodontal ligament stem cells to lipopolysaccharide and calcium silicate-based materials.

Autor: Pedrosa MDS; University of São Paulo - USP, School of Dentistry, Department of Biomaterials and Oral Biology, São Paulo, SP, Brazil., Vilela HDS; University of São Paulo - USP, School of Dentistry, Department of Biomaterials and Oral Biology, São Paulo, SP, Brazil., Rahhal JG; University of São Paulo - USP, School of Dentistry, Department of Restorative Dentistry, São Paulo, SP, Brazil., Bueno NP; University of São Paulo- USP, School of Dentistry, Department of Oral and Maxillofacial Surgery, Prosthesis and Traumatology, São Paulo, SP, Brazil., Lima FS; University of São Paulo - USP, School of Dentistry, Department of Biomaterials and Oral Biology, São Paulo, SP, Brazil., Nogueira FN; University of São Paulo - USP, School of Dentistry, Department of Biomaterials and Oral Biology, São Paulo, SP, Brazil., Sipert CR; University of São Paulo - USP, School of Dentistry, Department of Restorative Dentistry, São Paulo, SP, Brazil.

Publikováno v: Brazilian dental journal [Braz Dent J] 2022 Mar-Apr; Vol. 33 (2), pp. 73-82.