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Autor:
S. Dakubu, R.P. Ekins
Publikováno v:
Analytical Biochemistry. 144:20-26
A model of the relationships between optimal fluorescence yield, Eu3+ ion concentration, and the concentration of beta-diketone in the determination of Eu3+ ion concentration in aqueous solutions as used in time-resolved fluoroimmunoassays was develo
Publikováno v:
IFAC Proceedings Volumes. 11:541-548
A control system approach to health care is developed, illustrated in the context of thyroid disease with the emphasis being placed upon patient management. This approach requires a compatible information handling system and the implementation of an
Publikováno v:
Molecular and Cellular Endocrinology. 8:271-282
Thyrotropin (TSH) secretion from isolated anterior pituitary cells has been studied using the technique of cell column perifusion. The consistency in secretory rate and temporal profiles of TSH output in response to stimulation illustrated that the s
Publikováno v:
Clinica Chimica Acta. 91:317-327
Cross-reaction of anti-3,3',5'-triiodothyronine (rT3) antisera with thyroxine has proved problematical in the development of radioimmunoassays for rT3. Results of experimental work with two antisera with differing specificities are presented which il
Autor:
R.P. Ekins, T.M. Jackson
Publikováno v:
Journal of Immunological Methods. 87:13-20
The sensitivity of any immunoassay is a complex function of the underlying physico-chemical basis of the technique and the size and source of ‘experimental’ errors. Analysis of this relationship emphasizes the distinction between competitive labe
Publikováno v:
Journal of Steroid Biochemistry. 3:289-304
Simple methods for the radioimmunoassay of aldosterone in serum and urine are described. The methods rely on an aldosterone antiserum raised in rabbits using aldosterone-3-oxime coupled to bovine serum albumin as antigen. Aldosterone is extracted fro
Publikováno v:
Clinical Biochemistry. 2:253-288
Summary 1. A saturation assay for serum thyroxine is described with particular reference to the attainment of highest assay sensitivity and precision. The technique provides an illustration of the application of theoretical precepts put forward by Ek
Publikováno v:
The Lancet. 300:253-256
An extraction and assay procedure for urine thyroxine (T4) has been modified to enable the parallel determination of tri-iodothyronine (T3) by radioimmunoassay. Urinary T3 excretion, over a 24-hour period, is similar in both healthy and inpatient eut