Zobrazeno 1 - 10
of 64
pro vyhledávání: '"R.A. Goldsby"'
Publikováno v:
Journal of Endocrinology. 115:283-288
Bovine FSH (bFSH) was used to immunize BALB/c mice. Spleen cells were fused to the SP 2/0 cell line to produce hybridomas that secreted monoclonal antibodies to bFSH. One of these antibodies (USDA-bFSH-MC28) was extensively characterized and found to
Publikováno v:
Journal of Dairy Science. 66:1547-1558
Fusion of murine myeloma cells with syngeneic spleen lymphocytes has led to development of hybridomas secreting antibodies. Hybrid cells retain the immortality and clonability of myeloma parents as well as the antibody-producing property of lymphocyt
Publikováno v:
Journal of Immunological Methods. 59:277-280
The solid-phase immunoassay commonly used to screen hybridoma supernatants at times gave falsely positive results. A solution-phase screening technique, which uses radiolabeled antigen and a solid-phase immunoadsorbent, is described. This technique o
Autor:
R.A. Goldsby, B.M. Kaufman
Publikováno v:
Journal of Immunological Methods. 54:1-7
Monoclonal antibodies specific for human cell surface antigens were used to develop a technique for the simultaneous analysis of several antigenic determinants. The procedure was used to measure the relative expression of specific cell antigens durin
Autor:
R.A. Goldsby, R.E. Breslow
Publikováno v:
Experimental Cell Research. 55:339-346
In this paper a new system of checking mutation frequencies of the incorporation variety for cells in culture was presented. The mutation rate of a mutation in the thymidine incorporating system was determined by the fluctuation test to be 2.6 × 10
Autor:
R.A. Goldsby, P.G. Heytler
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 97:162-164
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Publikováno v:
Veterinary immunology and immunopathology. 5(4)
Three bovine × murine hybridomas, which secrete bovine IgG1, IgG2 and IgM, respectively, were derived by the fusion of normal bovine B lymphocytes to the murine cell line SP-2/0. The hybridomas were initially selected by testing the culture supernat