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Akademický článek
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Publikováno v:
Phytochemistry. 23:2307-2311
From callus cultures of Cinchona ledgeriana seven known anthraquinones, purpurin, anthragallol-1,2-dimethylether, anthragallol-1,3-dimethylether, rubiadin, 1-hydroxy-2-hydroxymethylanthraquinone, 1-hydroxy-2-methylanthraquinone and morindone-5-methyl
Publikováno v:
Plant Cell, Tissue and Organ Culture. 4:61-73
Methods for induction and maintenance of callus as well as cell suspension cultures of Mucuna pruriens are described. The presence of 3- (3, 4-dihy droxyphenyl 1)-L-alanine (L-DOPA) in these cell suspension cultures was demonstrated by means of TLC a
Publikováno v:
Plant Science. 47:71-76
Evidence is presented that the β-carboline alkaloids norharman and harman are artifacts formed from L-tryptophan. Transfer of Cinchona ledgeriana suspension cultured cells to Zenk's alkaloid production medium (ZAP medium) resulted in cell death. No
A rapid method for determination of growth supporting and toxic hormone levels in plant cell culture
Autor:
Robert Verpoorte, A. Baerheim Svendsen, Robert van der Heijden, R. Wijnsma, G. W. J. Heijmeijer, P. A. A. Harkes
Publikováno v:
Journal of Tissue Culture Methods. 11:27-30
The use of an agar diffusion method for the optimization of the phytohormone concentrations in growth media for plant cell cultures was studied. The method allows a rapid determination of both growth-supporting levels as well as toxic levels of the p
Publikováno v:
Phytochemistry. 25:1123-1126
From callus cultures of Cinchona pubescens seven known anthraquinones, alizarin-2-methylether, anthragallol-1,2-dimethylether, purpurin, purpurin-1-methylether, 1-hydroxy-2-hydroxymethylanthraquinone, 2-hydroxy-1,3,4-trimethoxyanthraquinone and 2,5-(
Publikováno v:
Journal of Chromatography A. 396:287-295
A method is reported for the determination of alkaloids in cell cultures of Tabernaemontana species. The alkaloids were isolated by means of C 8 pre-concentration columns. After recovery they were analysed on a Phenyl type of column using phosphate b
Autor:
Theodore Nsengiyaremge, Leny Krijbolder, Kees R. Libbenga, Robert Verpoorte, R. Wijnsma, P. A. A. Harkes
Publikováno v:
Plant Cell, Tissue and Organ Culture. 4:199-214
Using the methods reported by De Fossard et al. (11) the influence of various media constituents on the growth and the alkaloid and anthraquinone production in Cinchona ledgeriana callus cultures was studied. Growth and indole alkaloid production (e.
Autor:
Robert Verpoorte, R.W. van der Heijden, T.A. van Beek, A. Baerheim Svendsen, R. Wijnsma, A.A. Harkes, R. L. Brouwer
Publikováno v:
Phytochemistry. 25:843-846
Fourteen indole alkaloids have been isolated from a callus culture of Tabernaemontana elegans . One of them is new, 3-oxo-isovoacangine, the others are the known isovoacangine, 3- R / S -hydroxy-isovoacangine, 3- R / S -hydroxy-coronaridine, isositsi