Zobrazeno 1 - 10
of 16
pro vyhledávání: '"R. Termaat"'
Publikováno v:
AIChE Journal. 57:1912-1918
Large DNA constructs can be synthesized from smaller oligonucleotides using the polymerase chain reaction. The set of oligonucleotides should be designed so that the melting temperature amongst oligonucleotide hybridization pairs do not vary greatly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45580c85ebad8458a89770ed13fe4ead
https://doi.org/10.1002/aic.12410
https://doi.org/10.1002/aic.12410
Publikováno v:
Estuarine Coastal and Shelf Science, 53(3), 297-306. Academic Press
Estuarine, coastal and shelf science
Estuarine, Coastal and Shelf Science, 53, 297-306. Academic Press Inc.
Estuarine, coastal and shelf science
Estuarine, Coastal and Shelf Science, 53, 297-306. Academic Press Inc.
Three years of combined physical and biological measurements in the Dollard tidal basin showed that in the early spring of 1996, suspended particulate matter (SPM) concentrations were low compared to observations in spring 1995 and 1997. Since in 199
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56fcf9bb5be905a266fbc6241cbce022
https://doi.org/10.1006/ecss.2001.0764
https://doi.org/10.1006/ecss.2001.0764
Autor:
Hendrik J. Viljoen, Joel R. Termaat, Christine S. Booth, Scott E. Whitney, Elsje Pienaar, Tobias M. Louw
Publikováno v:
Chemical engineering science. 66(8)
Recently a theoretical analysis of PCR efficiency has been published by Booth et al. (2010). The PCR yield is the product of three efficiencies: (i) the annealing efficiency is the fraction of templates that form binary complexes with primers during
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::125c41a2c9eb16177fb6c84a0b3bc165
https://pubmed.ncbi.nlm.nih.gov/21822325
https://pubmed.ncbi.nlm.nih.gov/21822325
Autor:
Hendrik J. Viljoen, Scott E. Whitney, Joel R. Termaat, Elsje Pienaar, Tobias M. Louw, Christine S. Booth
Publikováno v:
Chemical engineering science. 65(17)
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology such as gene expression studies, mutation detection, forensic analysis and pathogen detection. Increasingly, quantitative real time PCR is used to as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bf10ba3c45cc92fe1ba65b386d8eb54
https://pubmed.ncbi.nlm.nih.gov/21799540
https://pubmed.ncbi.nlm.nih.gov/21799540
Publikováno v:
Journal of chromatographic science. 48(2)
Polymerase chain assembly (PCA) is a powerful tool for basic biological research and biotechnology applications. During the last several years, major advances have been made in de novo gene synthesis. However, there is still a need for fast and repro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77d381044b3b69dac3b26157cc345cbb
https://pubmed.ncbi.nlm.nih.gov/20109289
https://pubmed.ncbi.nlm.nih.gov/20109289
Publikováno v:
Journal of microbiological methods. 79(3)
Polymerase chain assembly (PCA) is a technique used to synthesize genes ranging from a few hundred base pairs to many kilobase pairs in length. In traditional PCA, equimolar concentrations of single stranded DNA oligonucleotides are repeatedly hybrid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b08db8d75b4fc64159f1720c8c378f88
https://pubmed.ncbi.nlm.nih.gov/19799938
https://pubmed.ncbi.nlm.nih.gov/19799938
Akademický článek
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Publikováno v:
Journal of immunological methods. 139(1)
The anti-dsDNA ELISA is probably one of the most popular techniques for determining antibody reactivity towards dsDNA, since this assay system has proven high sensitivity and is easy to perform. An important difference from other ELISA systems is the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::22895d1c128c52b3446539940a9f7688
https://pubmed.ncbi.nlm.nih.gov/2040819
https://pubmed.ncbi.nlm.nih.gov/2040819
Publikováno v:
BMC Medical Genetics
Background Most PCR-based diagnostics are still considered time- and labor-intensive due to disparate purification, amplification, and detection steps. Advancements in PCR enzymes and buffer chemistry have increased inhibitor tolerance, facilitating
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46e4e3cf39e371058a9c6e1addc7073b
Akademický článek
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