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Akademický článek

A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria

Autor: Markus de Raad, Yifan V. Li, Jennifer V. Kuehl, Peter F. Andeer, Suzanne M. Kosina, Andrew Hendrickson, Nicholas R. Saichek, Amber N. Golini, La Zhen Han, Ying Wang, Benjamin P. Bowen, Adam M. Deutschbauer, Adam P. Arkin, Romy Chakraborty, Trent R. Northen

Publikováno v: Frontiers in Microbiology, Vol 13 (2022)

Exometabolomics is an approach to assess how microorganisms alter, or react to their environments through the depletion and production of metabolites. It allows the examination of how soil microbes transform the small molecule metabolites within thei

Externí odkaz: https://doaj.org/article/2582858633924c35a57655987a2a02c2

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Untangling the sequence of events during the S 2 → S 3 transition in photosystem II and implications for the water oxidation mechanism

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, vol 117, iss 23
Proceedings of the National Academy of Sciences of the United States of America

Significance A new bridging oxygen ligand is incorporated between one of the Mn atoms and Ca in the Mn4Ca cluster during the transition from the one-photon induced S2 intermediate state to the two-photon induced S3 state in the catalytic water oxidat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03e9fc4b2308bbb70bbaa994da4af371
https://doi.org/10.1073/pnas.2000529117

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A defined medium based on R2A for cultivation and exometabolite profiling of soil bacteria

Autor: Benjamin P. Bowen, Amber Golini, Peter F. Andeer, Ying Wang, Romy Chakraborty, Suzanne M. Kosina, Yifan Li, Trent R. Northen, Markus de Raad, La Zhen Han, Nicholas R Saichek

Author(s): de Raad, Markus; Li, Yifan; Andeer, Peter; Kosina, Suzanne M; Saichek, Nicholas R; Golini, Amber; Han, La Zhen; Wang, Ying; Bowen, Benjamin P; Chakraborty, Romy; Northen, Trent R | Abstract: SummaryExometabolomics is an approach to assess

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16d9441165b760b74ad9a2183d005973
https://escholarship.org/uc/item/7hj7q67x

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Conservation of isolate substrate preferences in mixed communities revealed through ribosomal marker protein profiling

Autor: Trent R. Northen, Ying Wang, Benjamin P. Bowen, Nicholas R Saichek, Suzanne M. Kosina, Romy Chakraborty

Assessment of structure-function relationships is a central theme in microbial ecology. However, the degree that isolate metabolic activities are conserved in communities remains unclear. This is because tracking population dynamics and substrate par

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2fee9a78daa09e1754d715192200bac3
https://doi.org/10.1101/2021.05.12.443848

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Comparison of metal oxide catalysts for pyrolytic MOLI–MS bacterial identification

Autor: Nicholas R. Saichek, Peter de B. Harrington, Kent J. Voorhees, Kirk R. Jensen, Christopher Cox

Publikováno v: Journal of Analytical and Applied Pyrolysis. 113:78-83

Six metal oxide catalysts were investigated for effective cleavage and laser ionization of bacterial cell wall phospholipid extracts. Calcium oxide has been utilized in the past for this application, but suffered from degradation of catalytic activit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ad7e1fcfe9011e81fee8e12d82a1d0cc
https://doi.org/10.1016/j.jaap.2014.10.016

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Strain-level Staphylococcus differentiation by CeO2-metal oxide laser ionization mass spectrometry fatty acid profiling

Autor: Kent J. Voorhees, Nicholas R. Stambach, Seungki Kim, Nicholas R. Saichek, Christopher Cox, Peter de B. Harrington

Publikováno v: BMC microbiology, vol 16, iss 1
BMC Microbiology

Background The Staphylococcus genus is composed of 44 species, with S. aureus being the most pathogenic. Isolates of S. aureus are generally susceptible to β-lactam antibiotics, but extensive use of this class of drugs has led to increasing emergenc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::157af7aabbf0095a9cbeaf957b9ab948
https://doi.org/10.1186/s12866-016-0658-y

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Strain-level bacterial identification by CeO2-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods

Autor: Nicholas R. Saichek, Kent J. Voorhees, Christopher Cox, Kirk R. Jensen

Publikováno v: Scientific Reports

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid approach for clinical bacterial identification. However, current protein-based commercial bacterial ID methods fall short when differen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83e5933e8e629a256857f5984721d3c7

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Rapid

Autor: Christopher R, Cox, Nicholas R, Saichek, Herbert P, Schweizer, Kent J, Voorhees

Publikováno v: Bacteriophage

Phage amplification detected by MALDI-TOF MS was investigated for rapid and simultaneous Burkholderia pseudomallei identification and ceftazidime resistance determination. B. pseudomallei ceftazidime susceptible and resistant ΔpurM mutant strains Bp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c8cca6f61becf740c33c7ba01ca828ca
https://pubmed.ncbi.nlm.nih.gov/25050191

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