Zobrazeno 1 - 10
of 20
pro vyhledávání: '"R. S. Canseco"'
Autor:
R. S. Canseco, William H. Velander, Raymond L. Page, Christopher G. Russell, John L. Johnson, F.C. Gwazdauskas
Publikováno v:
Transgenic Research. 4:12-17
The polymerase chain reaction (PCR) technique was used to detect a whey acidic protein (WAP) gene and transgene presence in mouse ova cultured to various stages of development after pronuclear microinjection at the one-cell stage. The PCR technique d
Autor:
John R. Gibbons, John L. Johnson, Christopher G. Russell, D. R. Notter, William H. Velander, R. S. Canseco, Rebecca L. Krisher, F.C. Gwazdauskas
Publikováno v:
Transgenic Research. 3:226-231
The effect of DNA microinjection at various times after in vitro insemination on DNA detection and survival rates of bovine embryos was investigated. Oocytes were inseminated 24 h after maturation with frozen/thawed semen prepared with a Percoll sepa
Autor:
Rebecca L. Krisher, R. S. Canseco, F.C. Gwazdauskas, R.E. Pearson, William H. Velander, James W. Knight, M. A. Hajdu
Publikováno v:
Journal of Animal Science. 72:1299-1305
A series of experiments evaluated development of porcine zygotes microinjected with DNA in three culture media and two incubation temperatures, from postpubertal and prepubertal donors, and between zygotes injected with DNA into the pronucleus and th
Autor:
F.C. Gwazdauskas, William H. Velander, M. L. McGilliard, John L. Johnson, R. S. Canseco, J. L. Page, Amy E.T. Sparks, Christopher G. Russell
Publikováno v:
Animal Reproduction Science. 35:1-7
Bovine morulae (n = 94; d 6–6.5) were collected nondashsurgically from superovulated dairy cows (n=22) to assess embryo viability in vitro after bisection or biopsy and compare the efficiency of polymerase chain reaction (PCR) amplification of 18S
Autor:
William H. Velander, R. S. Canseco, John L. Johnson, Francis C. Gwazdauskas, Christopher G. Russell, Amy E.T. Sparks, H D Moll
Publikováno v:
Journal of Dairy Science. 77:718-724
In vivo fertilized embryos were surgically collected from superovulated dairy cows to evaluate microinjection on embryo development and utilized the polymerase chain reaction technique for selection of transgenic embryos. Seventy-two percent of the e
Autor:
B. L. Williams, A.E.T. Sparks, R. S. Canseco, J. W. Knight, J. L. Johnson, W. H. Velander, R. L. Page, W. N. Drohan, J. M. Young, R. E. Pearson, T. D. Wilkins, F. C. Gwazdauskas
Publikováno v:
Journal of Animal Science. 70:2207-2211
The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and
Publikováno v:
Journal of Dairy Science. 74:1815-1820
Bovine morulae (d 6) were used to evaluate embryonic development in a deproteinized hemodialysate, agar embedding, and in the uterus of the immature mouse. Agar-embedded embryos were cultured in Ham's F-10 and 10% steer serum either (treatment 1) imm
Publikováno v:
Reproduction in domestic animals = Zuchthygiene. 37(6)
The most important point in embryo transfer success is the evaluation of the stage of development and quality of embryos. Therefore, the purpose of this study was to compare the morphological evaluation of embryos using stereoscopy, light microscopy
Autor:
M A, Hajdu, J W, Knight, R S, Canseco, R L, Krisher, W H, Velander, R E, Pearson, F C, Gwazdauskas
Publikováno v:
Journal of animal science. 72(5)
A series of experiments evaluated development of porcine zygotes microinjected with DNA in three culture media and two incubation temperatures, from postpubertal and prepubertal donors, and between zygotes injected with DNA into the pronucleus and th
Autor:
Amy E.T. Sparks, Raymond L. Page, William H. Velander, William N. Drohan, John L. Johnson, R.E. Pearson, R. S. Canseco, F.C. Gwazdauskas, Christopher G. Russell
Publikováno v:
Transgenic research. 3(1)
Litter size of DNA microinjected zygotes is lower than for non-manipulated zygotes. The rate of embryonic and fetal survival in early, mid and late gestation was determined to assess whether DNA integration was responsible for embryonic losses. Also,