Zobrazeno 1 - 10
of 16
pro vyhledávání: '"R. Robert Balcarcel"'
Autor:
Yuansheng Yang, R. Robert Balcarcel
Publikováno v:
BioTechniques, Vol 36, Iss 2, Pp 286-295 (2004)
In this report, we describe a method for the quantitative determination of carbon dioxide production rates of mammalian cells. Custom-made, reusable, optically clear plugs are used to seal the wells of a 24-well plate. These plugs prevent the loss of
Externí odkaz:
https://doaj.org/article/a5e179d08c514231b6cb6dd0ee3b2ef9
Publikováno v:
The Canadian Journal of Chemical Engineering. 99:2570-2583
Autor:
Victoria C. Tai, Nancy Van Houten Fisher, Megan M. Suhoski, Carl H. June, Richard G. Carroll, Xiaochuan Shan, Tatiana N. Golovina, Tatiana Mikheeva, Bruce L. Levine, Bruce R. Blazar, Nicole A. Aqui, Ronghua Liu, James L. Riley, R. Robert Balcarcel, Noel L. Warner
Publikováno v:
The Journal of Immunology. 181:2855-2868
The costimulatory requirements required for peripheral blood T regulatory cells (Tregs) are unclear. Using cell-based artificial APCs we found that CD28 but not ICOS, OX40, 4-1BB, CD27, or CD40 ligand costimulation maintained high levels of Foxp3 exp
Publikováno v:
Journal of Applied Toxicology. 25:310-317
Chloroform is a non-genotoxic compound that is present in drinking water and ambient air as a result of water chlorination but whose carcinogenic mechanism in humans is unknown. Chloroform targets the liver in humans, where cytochrome P-450-dependent
Autor:
Yuansheng Yang, R. Robert Balcarcel
Publikováno v:
ASSAY and Drug Development Technologies. 2:353-362
A 96-well plate assay has been devised for estimation of sublethal metabolic activity for compounds administered to in vitro cell cultures during 6- and 24-h exposures. This screen combines a resazurin reduction assay with lactate production and gluc
Publikováno v:
Biotechnology and Bioengineering. 76:1-10
Rapamycin was used as a medium additive to slow the progression of CRL 1606 hybridomas through the cell cycle, under the hypothesis that such a modulation might reduce cell death. Cell cycle distributions for CRL hybridomas in the G1 phase of the cel
Autor:
Gregg B. Nyberg, Gregory Stephanopoulos, Daniel I. C. Wang, R. Robert Balcarcel, Brian D. Follstad
Publikováno v:
IBM Journal of Research and Development. 44:770-783
Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligos
Publikováno v:
Biotechnology and Bioengineering. 63:675-683
Physiological state multiplicity was observed in continuous cultures of the hybridoma cell line ATCC CRL-1606 cultivated in glutamine-limited steady state chemo-stats. At the same dilution rate (0.04 h -1 ), two physiologically different cultures wer
Autor:
Gregory Stephanopoulos, Gregg B. Nyberg, Daniel I. C. Wang, Brian D. Follstad, R. Robert Balcarcel
Publikováno v:
Biotechnology and Bioengineering. 62:336-347
Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligos