Výsledky vyhledávání - "R. R. Burgess"

An Overview of a Feasibility Study for the Production of Industrial Enzymes in Transgenic Alfalfaa

Autor: M. N. Shahan, S. Austin, R. G. Koegel, R. J. Straub, E. T. Bingham, D. E. Mathews, R. R. Burgess

Publikováno v: Annals of the New York Academy of Sciences. 721:234-244

The overall aims of the research are to develop genetically engineered alfalfa producing high levels of industrially important enzymes and to develop rapid methods for extracting and purifying these enzymes from alfalfa juice. Using a reporter gene b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e0cbc78b41e0083c9ae66dfd21ec7a4
https://doi.org/10.1111/j.1749-6632.1994.tb47395.x

Zobrazit plný text záznamu

Identification of polyol-responsive monoclonal antibodies for use in immunoaffinity chromatography

Autor: N E, Thompson, R R, Burgess

Publikováno v: Current protocols in molecular biology.

One of the limitations of immunoaffinity chromatography as been that high-affinity antigen-antibody complexes are difficult to dissociate, often leading to inactivation of the protein product during elution from the immobilized antibody. As described

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::394d0aa3c14e2435a9295e1d86f47f76
https://pubmed.ncbi.nlm.nih.gov/18265073

Zobrazit plný text záznamu

Chlamydia trachomatis RNA polymerase major sigma subunit. Sequence and structural comparison of conserved and unique regions with Escherichia coli sigma 70 and Bacillus subtilis sigma 43

Autor: N E Thompson, Jane E. Koehler, Richard S. Stephens, R R Burgess

Publikováno v: Journal of Biological Chemistry. 265:13206-13214

We identified and sequenced the gene for the Chlamydia trachomatis RNA polymerase major sigma subunit. The gene encodes a 66,141-dalton protein (sigma 66), intermediate in size between the major sigma subunits of Escherichia coli (sigma 70) and Bacil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::59228ad91719eccaf5134863846eea9f
https://doi.org/10.1016/s0021-9258(19)38286-9

Zobrazit plný text záznamu

Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins

Autor: R R, Burgess, T M, Arthur, B C, Pietz

Publikováno v: Methods in enzymology. 328

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::50c5308597404de9d50c190c8508826c
https://pubmed.ncbi.nlm.nih.gov/11075344

Zobrazit plný text záznamu

Immobilization of manganese peroxidase from Lentinula edodes and its biocatalytic generation of MnIII-chelate as a chemical oxidant of chlorophenols

Autor: A C, Grabski, H J, Grimek, R R, Burgess

Publikováno v: Biotechnology and bioengineering. 60(2)

Manganese peroxidase (MnP) purified from commercial cultures of Lentinula edodes was covalently immobilized through its carboxyl groups using an azlactone-functional copolymer derivatized with ethylenediamine and 2-ethoxy-1-ethoxycarbonyl-1,2-dihydro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::45bcc374808bf3e91a6bc730b63919e6
https://pubmed.ncbi.nlm.nih.gov/10099422

Zobrazit plný text záznamu

Use of asymmetric PCR to generate long primers and single-stranded DNA for incorporating cross-linking analogs into specific sites in a DNA probe

Autor: R R Burgess, Christine I. Wooddell

Publikováno v: Genome research. 6(9)

Photoactivatable DNA analogs have been incorporated enzymatically into DNA and used to map the locations of polypeptides in protein complexes bound to DNA. We have developed a procedure for generating long primers from short oligodeoxyribonucleotides

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f8cfb76ff84d5d4e38c888f9e5ed0e0
https://pubmed.ncbi.nlm.nih.gov/8889557

Zobrazit plný text záznamu

Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl

Autor: R R, Burgess

Publikováno v: Methods in enzymology. 273

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b3f48d808580a40028a7e17328986eee
https://pubmed.ncbi.nlm.nih.gov/8791607

Zobrazit plný text záznamu

Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies

Autor: N E, Thompson, R R, Burgess

Publikováno v: Methods in enzymology. 274

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6d8c23a7b1b6d75abbc45736bcc3513c
https://pubmed.ncbi.nlm.nih.gov/8902829

Zobrazit plný text záznamu

Tagetitoxin inhibition of RNA polymerase III transcription results from enhanced pausing at discrete sites and is template-dependent

Autor: T H, Steinberg, R R, Burgess

Publikováno v: The Journal of biological chemistry. 267(28)

In yeast nuclear extracts, tagetitoxin inhibition of RNA polymerase III promoter-directed single- and multiple-round transcription is characterized by pausing or stalling of the elongation complex at several discrete points on the template. Paused te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6e6e48ca4b8560c1708ca2b9ae4feba7
https://pubmed.ncbi.nlm.nih.gov/1400338

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání