Metal-based affinity separation of alpha- and gamma-chymotrypsin and thermal stability analysis of isolates

Autor: S L, Sagar, R R, Beitle, M M, Ataai, M M, Domach

Publikováno v: Bioseparation. 3(5)

Chymotrypsin preparations are contaminated by autolysis products and other post-translational products derived from the zymogen. Some prevalent contaminants are difficult to detect with activity assays and molecular weight separation. However, our di

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::71d3698217f115f87d6ed65ec437d590
https://pubmed.ncbi.nlm.nih.gov/1369428

A redesigned Escherichia coli triosephosphate isomerase restores growth properties in a bacterial strain useful for Immobilized Metal Affinity Chromatography (IMAC).

Autor: Haley R; Ralph E. Martin Department of Chemical Engineering, 3202 Bell Engineering Center, University of Arkansas, Fayetteville, AR 72701, United States., Fruchtl M; Boston Mountain Biotech, LLC 534W Research Center Blvd, Suite 254, Fayetteville, AR 72701, United States., Brune EM; Boston Mountain Biotech, LLC 534W Research Center Blvd, Suite 254, Fayetteville, AR 72701, United States., Ataai M; Department of Chemical and Petroleum Engineering, University of Pittsburgh, 1249 Benedum Hall, Pittsburgh, PA 15261, United States., Henry R; Department of Biological Sciences, 601 Science Engineering, University of Arkansas, Fayetteville, AR 72701, United States., Beitle R; Ralph E. Martin Department of Chemical Engineering, 3202 Bell Engineering Center, University of Arkansas, Fayetteville, AR 72701, United States. Electronic address: rbeitle@uark.edu.

Publikováno v: Journal of biotechnology [J Biotechnol] 2014 Oct 20; Vol. 188, pp. 48-52. Date of Electronic Publication: 2014 Jul 26.