Zobrazeno 1 - 10
of 29
pro vyhledávání: '"R. P. Haugland"'
Publikováno v:
Journal of Histochemistry & Cytochemistry. 43:907-915
The fungal metabolite brefeldin A (BFA) interferes with vesicular trafficking in most animal cells. To gain insight into the mechanism of BFA action, we esterified it to the fluorophore, boron dipyromethene difluoride (BODIPY). BODIPY-BEA localized p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48f205d5df771f3a79ad1842e37eba6c
https://doi.org/10.1177/43.9.7543914
https://doi.org/10.1177/43.9.7543914
Publikováno v:
Journal of Histochemistry & Cytochemistry. 41:313-317
We describe here the in situ detection of alkaline phosphatase (APase) activity with a new fluorogenic substrate, 2-(5'-chloro-2'-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (CPPCQ). CPPCQ is very soluble and colorless. APase converts it into
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05519b2169b768fa678931c45a0f8617
https://doi.org/10.1177/41.2.8419466
https://doi.org/10.1177/41.2.8419466
Autor:
John Bernardo, Richard J. Roman, G S Strohmeier, Richard D. Diamond, Elizabeth R. Simons, Tova Meshulam, Haya Herscovitz, David A. Casavant, R P Haugland
Publikováno v:
Journal of Biological Chemistry. 267:21465-21470
The interrelationships between activation of phospholipases and neutrophil stimulus-induced Ca2+ responses remain unclear. We report here that immune complexes activate a phosphatidylcholine-specific phospholipase A in a neutrophil only after the cyt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::41bab20670a632c44fd0841c09e7e2a3
https://doi.org/10.1016/s0021-9258(19)36632-3
https://doi.org/10.1016/s0021-9258(19)36632-3
Publikováno v:
The Journal of Cell Biology
A series of ceramide analogues bearing the fluorophore boron dipyrromethene difluoride (BODIPY) were synthesized and evaluated as vital stains for the Golgi apparatus, and as tools for studying lipid traffic between the Golgi apparatus and the plasma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4e2579b4a05db7936f72ae9af097bf9
https://doi.org/10.1083/jcb.113.6.1267
https://doi.org/10.1083/jcb.113.6.1267
Autor:
J Whitaker, Ira Pastan, R P Haugland, S J Currier, Mark C. Willingham, Michael M. Gottesman, Franz Thiebaut
Publikováno v:
Journal of Histochemistry & Cytochemistry. 38:685-690
Multidrug-resistant cells contain a plasma membrane efflux pump, the multidrug transporter, which actively expels certain hydrophobic drugs from the cytosol to the cell exterior. These drugs are usually positively charged at physiological pH. Because
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86b86fed082538f5a5050b29e44ed86c
https://doi.org/10.1177/38.5.1692055
https://doi.org/10.1177/38.5.1692055
Autor:
T H, Steinberg, K, Pretty On Top, K N, Berggren, C, Kemper, L, Jones, Z, Diwu, R P, Haugland, W F, Patton
Publikováno v:
Proteomics. 1(7)
The fluorescent hydrazide, Pro-Q Emerald 300 dye, may be conjugated to glycoproteins by a periodic acid Schiff's (PAS) mechanism. The glycols present in glycoproteins are initially oxidized to aldehydes using periodic acid. The dye then reacts with t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1202a49f8f5a315eae02becc0ba25a62
https://pubmed.ncbi.nlm.nih.gov/11503209
https://pubmed.ncbi.nlm.nih.gov/11503209
Publikováno v:
Electrophoresis. 22(5)
A two-color fluorescence detection method is described based upon covalently coupling the succinimidyl ester of BODIPY FL-X to proteins immobilized on poly(vinylidene difluoride) (PVDF) membranes, followed by detection of target proteins using the fl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::baf15d32c4936543f0de59e332e0d528
https://pubmed.ncbi.nlm.nih.gov/11332758
https://pubmed.ncbi.nlm.nih.gov/11332758
Autor:
K, Berggren, E, Chernokalskaya, T H, Steinberg, C, Kemper, M F, Lopez, Z, Diwu, R P, Haugland, W F, Patton
Publikováno v:
Electrophoresis. 21(12)
SYPRO Ruby dye is a permanent stain comprised of ruthenium as part of an organic complex that interacts noncovalently with proteins. SYPRO Ruby Protein Gel Stain provides a sensitive, gentle, fluorescence-based method for detecting proteins in one-di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::fe634c8c3252c705d37d647834c4bc6f
https://pubmed.ncbi.nlm.nih.gov/10939466
https://pubmed.ncbi.nlm.nih.gov/10939466
Autor:
T H, Steinberg, E, Chernokalskaya, K, Berggren, M F, Lopez, Z, Diwu, R P, Haugland, W F, Patton
Publikováno v:
Electrophoresis. 21(3)
SYPRO Ruby IEF Protein Gel Stain is an ultrasensitive, luminescent stain optimized for the analysis of protein in isoelectric focusing gels. Proteins are stained in a ruthenium-containing metal complex overnight and then rinsed in distilled water for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4dea03a7b74699efb2f52479e01ff970
https://pubmed.ncbi.nlm.nih.gov/10726748
https://pubmed.ncbi.nlm.nih.gov/10726748
Publikováno v:
Cytometry. 33(4)
We describe pairs of fluorochromes for use with the 407-nm line of a violet-light-enhanced krypton ion laser. These fluorochromes and a previously described violet-light-excited reporter variant, GFP-Vex, fall into two emission classes: blue for Casc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::43fef1f2ddd451d489bfaffd66a8ffc1
https://pubmed.ncbi.nlm.nih.gov/9845438
https://pubmed.ncbi.nlm.nih.gov/9845438