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Carbonic anhydrase inhibitors: The very weak inhibitors dithiothreitol, β-mercaptoethanol, tris(carboxyethyl)phosphine and threitol interfere with the binding of sulfonamides to isozymes II and IX

Autor: Claudiu T. Supuran, Mikaela Lindfors, Seppo Parkkila, Markku S. Kulomaa, Henri R. Nordlund, Andrea Scozzafava, Alessio Innocenti, Mika Hilvo

Publikováno v: Innocenti, A, Hilvo, M, Scozzafava, A, Lindfors, M, Nordlund, H R, Kulomaa, M S, Parkkila, S & Supuran, C T 2008, ' Carbonic anhydrase inhibitors: The very weak inhibitors dithiothreitol, β-mercaptoethanol, tris(carboxyethyl)phosphine and threitol interfere with the binding of sulfonamides to isozymes II and IX ', Bioorganic and Medicinal Chemistry Letters, vol. 18, no. 6, pp. 1898-1903 . https://doi.org/10.1016/j.bmcl.2008.02.008

The inhibition of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1) with dithiothreitol, 2-mercaptoethanol, tris(carboxyethyl)phosphine (reducing agent frequently added to enzyme assay buffers) and threitol has been investigated. The agents were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85e4a116ccbfcf75f1a3abe35aab7adb
https://doi.org/10.1016/j.bmcl.2008.02.008

Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells

Autor: Sanna T.H. Uotila, Minna Kulmala, Markku S. Kulomaa, Olli H. Laitinen, Henri R. Nordlund, Nisse Kalkkinen

Publikováno v: Biochemical and Biophysical Research Communications. 336:232-238

The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins fused to it in insect cells. This fusion protein forms a unique tag, which serves as a mul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b32c4b82f24d80d8149567d652b152cb
https://doi.org/10.1016/j.bbrc.2005.08.095

Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli

Autor: Markku S. Kulomaa, Juha A. E. Määttä, Mark S. Johnson, Eevaleena J. Porkka, Heidi Kidron, Olli H. Laitinen, Tiina Paldanius, Tiina A. Salminen, Jarno Hörhä, Henri R. Nordlund, Tomi T. Airenne, Kari J. Airenne, Seppo Ylä-Herttuala, Vesa P. Hytönen, Niko J. Meltola

Publikováno v: Biochemical Journal. 384:385-390

Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin protein in the periplasmic space of Escherichia coli in the active form is described. Avidin was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a43168d1cc77721eb4295f961c573d9
https://doi.org/10.1042/bj20041114

Baculovirus capsid display: a novel tool for transduction imaging

Autor: Christian Oker-Blom, Varpu Marjomäki, Kari J. Airenne, Anssi J. Mähönen, Seppo Ylä-Herttuala, Markku S. Kulomaa, Jani K. Raty, Pasi Kankaanpää, Henri R. Nordlund, Pauliina Lehtolainen, Olli H. Laitinen, Sari P. Kukkonen

Publikováno v: Molecular Therapy. 8(5):853-862

Baculoviruses are enveloped insect viruses that can carry large quantities of foreign DNA in their genome. Baculoviruses have proved to be very promising gene therapy vectors but little is known about their transduction mechanisms in mammalian cells.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7787b8d122acdf83c03b51844094f54