Zobrazeno 1 - 10
of 39
pro vyhledávání: '"R. Michael Sramkoski"'
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e80861 (2013)
B cyclins regulate G2-M transition. Because human somatic cells continue to cycle after reduction of cyclin B1 (cycB1) or cyclin B2 (cycB2) by RNA interference (RNAi), and because cycB2 knockout mice are viable, the existence of two genes should be a
Externí odkaz:
https://doaj.org/article/da0c841e7c8e4951ab9ae54e6b516aad
Publikováno v:
PLoS ONE, Vol 7, Iss 7, p e38275 (2012)
Cytometry of asynchronous proliferating cell populations produces data with an extractable time-based feature embedded in the frequency of clustered, correlated events. Here, we present a specific case of general methodology for calculating dynamic e
Externí odkaz:
https://doaj.org/article/8f5e1168eaa74f8688181fbf0d8354b9
Publikováno v:
PLoS Computational Biology, Vol 7, Iss 2, p e1001077 (2011)
The timing of DNA synthesis, mitosis and cell division is regulated by a complex network of biochemical reactions that control the activities of a family of cyclin-dependent kinases. The temporal dynamics of this reaction network is typically modeled
Externí odkaz:
https://doaj.org/article/6f168a7685ca45c882d9473a45f23d90
Publikováno v:
Flow Cytometry Protocols ISBN: 9781493973446
Cell cycle cytometry and analysis are essential tools for studying cells of model organisms and natural populations (e.g., bone marrow). Methods have not changed much for many years. The simplest and most common protocol is DNA content analysis, whic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1aa6b0a76af0434692846899bf08d66b
https://doi.org/10.1007/978-1-4939-7346-0_11
https://doi.org/10.1007/978-1-4939-7346-0_11
Publikováno v:
Cell Cycle. 7:1285-1300
Cyclin B1 should have some rate limiting function for cell cycle progression. To test this, we measured the effect of siRNA-mediated depletion of cyclin B1 on mitotic entry and timing. We depleted cyclin B1 in HeLa and hTert-RPE1 cells to levels equi
Autor:
Sudesh Agrawal, A.R.M. Ruhul Amin, George R. Stark, Mukesh K. Agarwal, Vijay S. Thakur, Mark W. Jackson, James W. Jacobberger, Kedar Hastak, Rajib Paul, Munna L. Agarwal, R. Michael Sramkoski
Publikováno v:
Proceedings of the National Academy of Sciences. 105:6314-6319
p53-dependent G 1 and G 2 cell cycle checkpoints are activated in response DNA damage that help to maintain genomic stability. p53 also helps to protect cells from damage that occurs during S phase, for example, when the cells are starved for DNA pre
Autor:
Tammy Stefan, Keith Shults, Deena V. Soni, James W. Jacobberger, R. Michael Sramkoski, Phyllis S. Frisa
Publikováno v:
Cytometry Part A. :5-15
Many epitopes are phosphorylated during mitosis. These epitopes are useful biomarkers for mitotic cells. The most commonly used are MPM-2 and serine 10 of histone H3. Here we investigated the use of an antibody generated against a phospho peptide mat
Autor:
John J. Erickson, James W. Jacobberger, Brian T. Grimberg, Peter A. Zimmerman, R. Michael Sramkoski
Publikováno v:
Cytometry Part A. :546-554
The complex life cycle of Plasmodium falciparum (Pf) makes it difficult to limit infections and reduce the risk of severe malaria. Improved understanding of Pf blood-stage growth and development would provide new opportunities to evaluate and interfe
Autor:
Donny D. Licatalosi, Molly M. Hannigan, Qin Li, Sarah E. Grabinski, R. Michael Sramkoski, Leah L. Zagore, Thomas J. Sweet
Publikováno v:
Molecular and cellular biology. 35(23)
RNA binding proteins (RBPs) are increasingly recognized as essential factors in tissue development and homeostasis. The polypyrimidine tract binding (PTB) protein family of RBPs are important posttranscriptional regulators of gene expression. In the
Autor:
Erica M. Wood, Ian Woolley, James W. Kazura, Peter A. Zimmerman, John P. Miller, R. Michael Sramkoski
Publikováno v:
Immunohematology. 21:15-20
Flow cytometric methods commonly used to identify reticulocytes are of limited usefulness in malarious areas, since RNA staining also detects plasmodia. An important antigen expressed on reticulocytes is Duffy antigen receptor for chemokines (DARC, a