A simple method to determine changes in the affinity between HisF and HisH in the Imidazole Glycerol Phosphate Synthase heterodimer.

Autor: Vitor M Almeida, J Patrick Loria, Sandro R Marana

Publikováno v: PLoS ONE, Vol 17, Iss 4, p e0267536 (2022)

The bi-enzyme HisF-HisH heterodimer is part of the pathway that produces histidine and purines in bacteria and lower eukaryotes, but it is absent in mammals. This heterodimer has been largely studied probing the basis of the allosteric effects and th

Externí odkaz: https://doaj.org/article/099cdaed7ad743b18e8d4d2d85eb85e3

Structural and biochemical data of Trichoderma harzianum GH1 β-glucosidases

Autor: Renata N. Florindo, Valquiria P. Souza, Hemily S. Mutti, Lívia R. Manzine Margarido, Cesar Camilo, Sandro R. Marana, Igor Polikarpov, Alessandro S. Nascimento

Publikováno v: Data in Brief, Vol 15, Iss , Pp 340-343 (2017)

Here the statistics concerning X-ray data processing and structure refinement are given, together with the substrate preference analysis for ThBgl1 and ThBgl2. Finally, the analysis of the influence of temperature and pH on the activities of both enz

Externí odkaz: https://doaj.org/article/b7e90fa16300456a83b18ad374e71504

Search for independent (β/α)4 subdomains in a (β/α)8 barrel β-glucosidase.

Autor: Vitor M Almeida, Maira A Frutuoso, Sandro R Marana

Publikováno v: PLoS ONE, Vol 13, Iss 1, p e0191282 (2018)

Proteins that fold as (β/α)8 barrels are thought to have evolved from half-barrels that underwent duplication and fusion events. The evidence is particularly clear for small barrels, which have almost identical halves. Additionally, computational c

Externí odkaz: https://doaj.org/article/359e85f6f0a64201ac1e56101ee134ab

H2O2 release from the very long chain acyl-CoA dehydrogenase

Autor: Pâmela A.H.B. Kakimoto, Fábio K. Tamaki, Ariel R. Cardoso, Sandro R. Marana, Alicia J. Kowaltowski

Publikováno v: Redox Biology, Vol 4, Iss C, Pp 375-380 (2015)

Enhanced mitochondrial generation of oxidants, including hydrogen peroxide (H2O2), is related to a large number of pathological conditions, including diet-induced obesity and steatohepatosis. Indeed, we have previously shown that high fat diets incre

Externí odkaz: https://doaj.org/article/7f94a1bff3b64b02becf2d4f73aef63b

Molecular Dynamics Reveals Complex Compensatory Effects of Ionic Strength on the Severe Acute Respiratory Syndrome Coronavirus 2 Spike/Human Angiotensin-Converting Enzyme 2 Interaction

Autor: Chuck S. Farah, Cristiane R. Guzzo, Anacleto Silva de Souza, Jose D. Rivera, Henning Ulrich, Pingju Ge, Roberto Kopke Salinas, Vitor Medeiros Almeida, Robson Francisco de Souza, Sandro R. Marana

Publikováno v: The Journal of Physical Chemistry Letters
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

The SARS-CoV-2 pandemic has already killed more than one million people worldwide. To gain entry, the virus uses its Spike protein to bind to host hACE-2 receptors on the host cell surface and mediate fusion between viral and cell membranes. As initi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3491d1ba25cffc44c0ec011aaa13445f
https://doi.org/10.1021/acs.jpclett.0c02602