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pro vyhledávání: '"R. M. Sandri-Goldin"'
Autor:
R M, Sandri-Goldin
Publikováno v:
Current topics in microbiology and immunology. 259
Autor:
R M, Sandri-Goldin
Publikováno v:
Infectious agents and disease. 3(2-3)
The herpes simplex virus type 1 (HSV-1) immediate early (alpha) protein ICP27 is an essential regulatory protein that appears to be involved in a number of different processes during lytic viral infection. Viral mutants defective in ICP27 have a vari
Autor:
R. M. Sandri-Goldin
The alpha herpesviruses are an important group of viruses characterized by a short reproductive cycle, rapid destruction of the host cell, and the ability to replicate in a wide variety of host tissues. A key attribute of these viruses is the ability
Publikováno v:
Molecular and Cellular Biology. 1:743-752
The protoplast fusion technique of Schaffner (W. Schaffner, Proc. Natl. Acad. Sci. U.S.A. 77:2163-2167, 1980) has been adapted to introduce cloned herpes simplex virus genes into cultured mammalian cells. The technique involves digesting bacterial ce
Publikováno v:
Methods in enzymology. 101
Protoplast fusion is a highly efficient method for effecting gene transfer to cells in culture resulting in stable transformation at high frequency. A number of cell lines have been used successfully as recipients. There is no need to isolate and pur