Zobrazeno 1 - 10
of 14
pro vyhledávání: '"R. K. Niedenthal"'
Autor:
Ulrich Güldener, Linda Riles, Johannes H. Hegemann, R. K. Niedenthal, Sabine Klein, Mark Johnston
Publikováno v:
Yeast. 15:1775-1796
To begin genome-wide functional analysis, we analysed the consequences of deleting each of the 265 genes of chromosome VIII of Saccharomyces cerevisiae. For 33% of the deletion strains a growth phenotype could be detected: 18% of the genes are essent
Autor:
Johannes H. Hegemann, Susanne Heck, R. K. Niedenthal, Sabine Klein, Ursula Fleig, Ulrich Güldener
Publikováno v:
Yeast. 15:1009-1019
We have developed a simple, fast and reliable method for the analysis of genetic stability in budding yeast strains. The assay relies on our previous finding that cells expressing the green fluorescent protein (GFP) can be detected and counted by flo
Publikováno v:
Yeast. 12:773-786
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has attracted much attention as a tool to study a number of biological processes. This study describes the use of GFP as a vital reporter molecule for localization and expressio
Publikováno v:
Yeast. 12:505-514
In the framework of the European Union programme for sequencing the genome of Saccharomyces cerevisiae we have determined the nucleotide sequence of a region of 24,152 bp located on the left arm of chromosome XIV between the BNI1 and the POL2 genes.
Publikováno v:
Nucleic Acids Research. 22:2791-2800
The centromere and promoter factor Cpf1 binds centromere DNA element I found in all centromere DNAs from the yeast Saccharomyces cerevisiae. We analyzed thirty different point mutations in or around CEN6-CDEI (ATCACGTG) for their relative binding aff
Publikováno v:
Nucleic Acids Research. 21:4726-4733
The centromere complex is a multicomponent structure essential for faithful chromosome transmission. Here we show that the S. cerevisiae centromere protein Cpf1 bends centromere DNA element I (CDEI) with the bend angle ranging from 66 degrees to 71 d
Publikováno v:
Molecular and Cellular Biology. 11:5212-5221
In the yeast Saccharomyces cerevisiae, the complete information needed in cis to specify a fully functional mitotic and meiotic centromere is contained within 120 bp arranged in the three conserved centromeric (CEN) DNA elements CDEI, -II, and -III.
Publikováno v:
Molecular and Cellular Biology. 11:3545-3553
The centromere DNA element I (CDEI) is an important component of Saccharomyces cerevisiae centromere DNA and carries the palindromic sequence CACRTG (R = purine) as a characteristic feature. In vivo, CDEI is bound by the helix-loop-helix protein CPF1
Autor:
P. Meyer, Sabine Klein, H. Launhardt, Ulrich Güldener, M. Ramezani Rad, Gregor Jansen, R. K. Niedenthal, M.-L. Siegler, Klaus Werner Wolf, Johannes H. Hegemann, T. Munder, N. Wilhelm, A. Römer, Horst Feldmann, A. Röhmer, Friedrich K. Zimmermann, Peter Kötter, J. Vetter, Alfred Zollner, Dietmar Becher, R. Götz, A. Maierl, C. P. Hollenberg, W. Mewes, Albert Hinnen, Gertrud Mannhaupt, J. Kricke, Matthias Rose, Bernd Schäfer, T. Schuster, M. Hansen, K. D. Entian, W. Kramer
Publikováno v:
Moleculargeneral genetics : MGG. 262(4-5)
In a systematic approach to the study of Saccharomyces cerevisiae genes of unknown function, 150 deletion mutants were constructed (1 double, 149 single mutants) and phenotypically analysed. Twenty percent of all genes examined were essential. The vi
Publikováno v:
Yeast (Chichester, England). 15(10B)
We have developed a simple, fast and reliable method for the analysis of genetic stability in budding yeast strains. The assay relies on our previous finding that cells expressing the green fluorescent protein (GFP) can be detected and counted by flo