Zobrazeno 1 - 10
of 17
pro vyhledávání: '"R. H. Ashley"'
Autor:
K. A. Hayman, R. H. Ashley
Publikováno v:
The Journal of Membrane Biology. 136:191-197
Ion channels from sheep cardiac mitoplast (inverted inner mitochondrial membrane vesicle) preparations were incorporated into voltage-clamped planar lipid bilayers. A low-conductance anion channel (approximately 40 or approximately 85 pS in symmetric
Publikováno v:
The Journal of Membrane Biology. 136:181-190
Ion channels from sheep cardiac mitoplast (inverted inner mitochondrial membrane vesicle) preparations were incorporated into voltage-clamped planar lipid bilayers. The appearance of anion rather than cation channels could be promoted by exposing the
Autor:
Alan J. Williams, R H Ashley
Publikováno v:
The Journal of General Physiology
Single Ca2+ release channels from vesicles of sheep cardiac junctional sarcoplasmic reticulum have been incorporated into uncharged planar lipid bilayers. Single-channel currents were recorded from Ca2(+)-activated channels that had a Ca2+ conductanc
Publikováno v:
The FEBS journal. 274(24)
Chloride intracellular channels (CLICs) are soluble, signal peptide-less proteins that are distantly related to Omega-type glutathione-S-transferases. Although some CLICs bypass the classical secretory pathway and autoinsert into cell membranes to fo
Publikováno v:
The Biochemical journal. 359(Pt 1)
Mammalian chloride intracellular channel (CLIC) (p64-related) proteins are widely expressed, with an unusual dual localization as both soluble and integral membrane proteins. The molecular basis for their cellular localization and ion channel activit
Autor:
V, Shoshan-Barmatz, R H, Ashley
Publikováno v:
International review of cytology. 183
The fundamental biological process of Ca2+ signaling is known to be important in most eukaryotic cells, and inositol 1,2,5-trisphosphate and ryanodine receptors, intracellular Ca2+ release channels encoded by two distantly related gene families, are
Autor:
R H, Ashley
Publikováno v:
Essays in biochemistry. 30
Publikováno v:
European biophysics journal : EBJ. 23(4)
A K+ channel was incorporated into voltage-clamped planar lipid bilayers from bovine chromaffin granules and resealed granule membranes ("ghosts"). It was not incorporated from plasma membrane-rich fractions from the adrenal medulla. The channel had
Autor:
R. H. Ashley
Publikováno v:
The Journal of Membrane Biology. 111:179-189
Rat brain microsomal membranes were found to contain high-affinity binding sites for the alkaloid ryanodine (kd 3 nM, Bmax 0.6 pmol per mg protein). Exposure of planar lipid bilayers to microsomal membrane vesicles resulted in the incorporation, appa
Publikováno v:
Biochemical Journal. 219:149-158
The recently synthesized calcium indicator quin −2 was incorporated into synaptosomes from guinea-pig cerebral cortex following uptake and internal hydrolysis of quin −2 tetra-acetoxymethyl ester. Incubation in physiological media containing 1 mM