Zobrazeno 1 - 10
of 13
pro vyhledávání: '"R. Gray Huffman"'
Publikováno v:
Genome Biology, Vol 23, Iss 1, Pp 1-31 (2022)
Abstract Background Many biological processes, such as cell division cycle and drug resistance, are reflected in protein covariation across single cells. This covariation can be quantified and interpreted by single-cell mass spectrometry with suffici
Externí odkaz:
https://doaj.org/article/d160c19bb2534a74ada075541bdfd6d0
Autor:
Harrison Specht, Edward Emmott, Aleksandra A. Petelski, R. Gray Huffman, David H. Perlman, Marco Serra, Peter Kharchenko, Antonius Koller, Nikolai Slavov
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-27 (2021)
Abstract Background Macrophages are innate immune cells with diverse functional and molecular phenotypes. This diversity is largely unexplored at the level of single-cell proteomes because of the limitations of quantitative single-cell protein analys
Externí odkaz:
https://doaj.org/article/48c645d97c414b82abdbbbe683278eab
Autor:
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A. Petelski, David H. Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov
Publikováno v:
Nature Methods. 20:714-722
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth of protein quantification, especially for proteins and modifications of biological interest. Here, to simultaneously advance all these aims, we developed p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c2cbe6263ec39b9b43e5265aae1afe91
https://doi.org/10.1038/s41592-023-01830-1
https://doi.org/10.1038/s41592-023-01830-1
Autor:
R Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco di Gioia, Francesco Borriello, Harrison Specht, Jason Derks, Saad Khan, Luke Khoury, Edward Emmott, Aleksandra A. Petelski, David H Perlman, Jürgen Cox, Ivan Zanoni, Nikolai Slavov
Major aims of single-cell proteomics include increasing the consistency, sensitivity, and depth of protein quantification, especially for proteins and modifications of biological interest. To simultaneously advance all these aims, we developed priori
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5fa6871c587c9af505a64fa41b0578c6
https://doi.org/10.1101/2022.03.16.484655
https://doi.org/10.1101/2022.03.16.484655
Autor:
Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman, Matthew Willetts, Saad Khan, Harrison Specht, Markus Ralser, Vadim Demichev, Nikolai Slavov
Publikováno v:
Nature Biotechnology
Nat Biotechnol
Nat Biotechnol
Current mass-spectrometry methods enable high-throughput proteomics of large sample amounts, but proteomics of low sample amounts remains limited in depth and throughput. To increase the throughput of sensitive proteomics, we developed an experimenta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f911bd07567d45723e6e4b41df71965
Autor:
Harrison Specht, Edward Emmott, Aleksandra A. Petelski, R. Gray Huffman, David H. Perlman, Antonius Koller, Nikolai Slavov
Protocol for preparing single cells for mass-spec analysis by SCoPE2 as described by Specht et al., doi: 10.1101/665307 (2019) and Specht et al., Genome Biology, doi: 10.1186/s13059-021-02267-5 (2021). For a detailed protocol paper, see Petelski A, E
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c31f971b892eeedbf5c7d3a2910d4fb3
https://doi.org/10.17504/protocols.io.bwb3paqn
https://doi.org/10.17504/protocols.io.bwb3paqn
Autor:
R. Gray Huffman, Aleksandra A. Petelski, Andrew Leduc, Harrison Specht, Edward Emmott, David H. Perlman, Nikolai Slavov
Publikováno v:
Nature Protocols
Nat Protoc
Nat Protoc
Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a48a61879c63746354df9dec3b721bc1
https://doi.org/10.1101/2021.03.12.435034
https://doi.org/10.1101/2021.03.12.435034
Autor:
Specht, Harrison, Emmott, Edward, Petelski, Aleksandra A., R. Gray Huffman, Perlman, David H., Serra, Marco, Kharchenko, Peter, Koller, Antonius, Slavov, Nikolai
Additional file 2. Review history.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33db20fdf9850966209883332b1b15d0
Autor:
Aleksandra A. Petelski, R. Gray Huffman, Marco Serra, David H. Perlman, Edward Emmott, Harrison Specht, Antonius Koller, Peter V. Kharchenko, Nikolai Slavov
Publikováno v:
Genome Biology
GENOME BIOLOGY
Genome Biology, Vol 22, Iss 1, Pp 1-27 (2021)
GENOME BIOLOGY
Genome Biology, Vol 22, Iss 1, Pp 1-27 (2021)
Background Macrophages are innate immune cells with diverse functional and molecular phenotypes. This diversity is largely unexplored at the level of single-cell proteomes because of the limitations of quantitative single-cell protein analysis. Resul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f7a93211123bbbb5a22e0f715985ba7
http://europepmc.org/articles/PMC7839219
http://europepmc.org/articles/PMC7839219
Autor:
Harrison Specht, Edward Emmott, Aleksandra A. Petelski, David H. Perlman, Marco Serra, Peter V. Kharchenko, Antonius Koller, Nikolai Slavov, R. Gray Huffman
Macrophages are innate immune cells with diverse functional and molecular phenotypes. This diversity is largely unexplored at the level of single-cell proteomes because of limitations of quantitative single-cell protein analysis. To overcome this lim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba6c9a56eff1c2f51150d182bb8a7a74
http://livrepository.liverpool.ac.uk/3150797/1/SpechtSCoPE2.pdf
http://livrepository.liverpool.ac.uk/3150797/1/SpechtSCoPE2.pdf