Zobrazeno 1 - 10
of 47
pro vyhledávání: '"R. F. Steiner"'
Autor:
R. F. Steiner, Jianqing Lan
Publikováno v:
Biochemical Journal. 274:445-451
Phosphofructokinase (PFK) is a calmodulin (CaM)-binding protein [Mayr & Heilmeyer (1983) FEBS Lett. 195, 51-57]. We found that troponin C (TnC), which is homologous to CaM, also binds PFK and affects PFK's catalytic activity, aggregation states and c
Publikováno v:
Journal of Biological Chemistry. 266:2327-2332
The ArsA protein, the catalytic component of the plasmid-encoded resistance system for removal of the toxic oxyanions arsenite, antimonite, and arsenate from bacterial cells, catalyzes oxyanion-stimulated ATP hydrolysis. Three lines of evidence sugge
Publikováno v:
The Journal of biological chemistry. 269(3)
The regulatory peptides Phk13 (301-327) and Phk5 (342-367) have been synthesized and their interaction with calmodulin studied. In the case of Phk13 modified forms were also synthesized in which a tryptophan group was placed at position 4 or 21, as w
Autor:
E, Bucci, R F, Steiner
Publikováno v:
Biophysical chemistry. 37(1-3)
When an allosteric macromolecular system is capable of existing in two conformations, both of which may be converted into energy-storing forms by the binding of a substrate or by the absorption of radiant energy, then a kinetic process may occur, suc
Autor:
R F Steiner, C G Caday
Publikováno v:
Journal of Biological Chemistry. 260:5985-5990
The dye "Stains-all" combines with calmodulin to yield a series of complex species whose absorption and circular dichroism spectra are sensitive to the presence of Ca2+ or Mg2+. At high dye:calmodulin ratios, the dominant complex formed is characteri
Autor:
R. F. Steiner, L. Marshall
Publikováno v:
Biopolymers. 24:547-563
Derivatives of calmodulin have been prepared in which tyrosine-99 and tyrosine-138 have been converted to their aminotyrosine derivatives. Significant changes in microenvironment occur in the presence of Ca2+. Both groups have a significant degree of
Autor:
A. J. McAlister, R. F. Steiner
Publikováno v:
Journal of Polymer Science. 24:105-123
Measurements of excited lifetime and of degree of polarization have been combined to yield values for the mean relaxation time of fluorescent conjugates of bovine serum albumin, egg albumin, and lysozyme. Evidence has been presented for the validity
Autor:
R. F. Steiner, H. Edelhoch
Publikováno v:
Journal of the American Chemical Society. 83:1435-1444
The rotational relaxation time of native thyroglobulin has been evaluated from polarization measurements of thyroglobulin coupled to a fluorescent dye. By treatment with detergent or urea the relaxation time of thyroglobulin was markedly reduced. The
Autor:
R. F. Steiner, Harold Edelhock
Publikováno v:
Journal of the American Chemical Society. 84:2139-2148
The effects of acid, alkali, anionic and cationic detergents, urea and guanidine on the relaxation times of rabbit anti-thyro-globulin antibody and bovine gamma globulin have been evaluated by means of the polarization of fluorescence technique. Both
Autor:
R. F. Steiner
Publikováno v:
The Journal of Chemical Physics. 32:215-221
A theory is developed for the hydrogen ion titration curve of doubly stranded helical polynucleotides for which the two strands are nonidentical and very long. It is shown that for pronounced sharpening to occur the binding (or dissociation) of a pro