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Akademický článek

Detecting RNA base methylations in single cells by in situ hybridization

Autor: Rohan T. Ranasinghe, Martin R. Challand, Kristina A. Ganzinger, Benjamin W. Lewis, Charlotte Softley, Wolfgang H. Schmied, Mathew H. Horrocks, Nadia Shivji, Jason W. Chin, James Spencer, David Klenerman

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)

Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific

Externí odkaz: https://doaj.org/article/681a36aec6e7448383e2cd2cf34e6586

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Akademický článek

The Bristol Sponge Microbiome Collection: A Unique Repository of Deep-Sea Microorganisms and Associated Natural Products

Autor: Sam E. Williams, Henry L. Stennett, Catherine R. Back, Kavita Tiwari, Jorge Ojeda Gomez, Martin R. Challand, Katharine R. Hendry, James Spencer, Angela E. Essex-Lopresti, Christine L. Willis, Paul Curnow, Paul R. Race

Publikováno v: Antibiotics, Vol 9, Iss 8, p 509 (2020)

The deep ocean is the largest habitat for life on Earth, though the microorganisms that occupy this unique environmental niche remain largely unexplored. Due to the significant logistical and operational challenges associated with accessing the deep

Externí odkaz: https://doaj.org/article/42ef5339cb4f48669f376071db162784

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Akademický článek

Cysteine methylation controls radical generation in the Cfr radical AdoMet rRNA methyltransferase.

Autor: Martin R Challand, Enrico Salvadori, Rebecca C Driesener, Christopher W M Kay, Peter L Roach, James Spencer

Publikováno v: PLoS ONE, Vol 8, Iss 7, p e67979 (2013)

The 'radical S-adenosyl-L-methionine (AdoMet)' enzyme Cfr methylates adenosine 2503 of the 23S rRNA in the peptidyltransferase centre (P-site) of the bacterial ribosome. This modification protects host bacteria, notably methicillin-resistant Staphylo

Externí odkaz: https://doaj.org/article/9f91cb55988f46ca9b499787a21f4947

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The Bristol Sponge Microbiome Collection:A unique repository of deep-sea microorganisms and associated natural products

Autor: Katharine R. Hendry, Paul R. Race, Martin R. Challand, Jorge Ojeda Gomez, Paul Curnow, Kavita Tiwari, James Spencer, Catherine R. Back, Sam E Williams, Christine L. Willis, Angela E. Essex-Lopresti, Henry L Stennett

Publikováno v: Williams, S, Stennett, H L, Back, C, Tiwari, K, Ojeda Gomez, J, Challand, M R, Hendry, K, Spencer, J, Essex-Lopresti, A, Willis, C L, Curnow, P & Race, P R 2020, ' The Bristol Sponge Microbiome Collection : A unique repository of deep-sea microorganisms and associated natural products ', Antibiotics, vol. 9, no. 8, 509 . https://doi.org/10.3390/antibiotics9080509
Antibiotics
Volume 9
Issue 8
Antibiotics, Vol 9, Iss 509, p 509 (2020)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99c91bb9b604734931706cef4a6c4668
https://research-information.bris.ac.uk/ws/files/247315525/antibiotics_09_00509_v2.pdf

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A Rieske oxygenase/epoxide hydrolase-catalysed reaction cascade creates oxygen heterocycles in mupirocin biosynthesis

Autor: Christopher Williams, Christine L. Willis, Luoyi Wang, Marc W. van der Kamp, Alice E. Parnell, Paul R. Race, Nurfarhanim A. Bakar, Martin R. Challand, Thomas J. Simpson, Matthew P. Crump

Publikováno v: Wang, L, Parnell, A, Williams, C, Bakar, N A, Challand, M R, van der Kamp, M W, Simpson, T J, Race, P R, Crump, M P & Willis, C L 2018, ' A Rieske oxygenase/epoxide hydrolase-catalysed reaction cascade creates oxygen heterocycles in mupirocin biosynthesis ', Nature Catalysis, vol. 1, no. 12, pp. 968-976 . https://doi.org/10.1038/s41929-018-0183-5

Oxygen heterocycles—in particular, tetrahydropyrans (THPs) and tetrahydrofurans—are common structural features of many biologically active polyketide natural products. Mupirocin is a clinically important antibiotic isolated from Pseudomonas fluor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bff88620e00752a79e16987aa148a9dc
https://doi.org/10.1038/s41929-018-0183-5

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Imaging rRNA Methylation in Bacteria by MR-FISH

Autor: Kristina A, Ganzinger, Martin R, Challand, James, Spencer, David, Klenerman, Rohan T, Ranasinghe

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2038

Methylation of RNA is normally monitored in purified cell lysates using next-generation sequencing, gel electrophoresis, or mass spectrometry as readouts. These bulk methods require the RNA from ~10

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::981695d5f67eb06552c3dbcaca8e47df
https://pubmed.ncbi.nlm.nih.gov/31407280

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Imaging rRNA Methylation in Bacteria by MR-FISH

Autor: James Spencer, Martin R. Challand, Rohan T. Ranasinghe, Kristina A. Ganzinger, David Klenerman

Publikováno v: Imaging Gene Expression ISBN: 9781493996735

Methylation of RNA is normally monitored in purified cell lysates using next-generation sequencing, gel electrophoresis, or mass spectrometry as readouts. These bulk methods require the RNA from ~104 to 107 cells to be pooled to generate sufficient m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1ebfa3ac6d7ee4fd2a98bc0ab6f4e056
https://doi.org/10.1007/978-1-4939-9674-2_7

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