Systematic investigation of CRISPR–Cas9 configurations for flexible and efficient genome editing in Corynebacterium glutamicum NRRL-B11474

Autor: Jim Vallandingham, Harmen M. van Rossum, Kedar G. Patel, Shawn Szyjka, Zach Serber, Jed Dean, R. Cameron Coates, Stephen Blaskowski

Publikováno v: Journal of Industrial Microbiology and Biotechnology. 46:187-201

This study details a reliable and efficient method for CRISPR–Cas9 genome engineering in the high amino acid-producing strain of Corynebacterium glutamicum, NRRL-B11474. Our investigation demonstrates that a plasmid-encoded single-guide RNA paired

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c282b32afcf08b0896929e498b5b61f
https://doi.org/10.1007/s10295-018-2112-7

Single cell genome amplification accelerates identification of the apratoxin biosynthetic pathway from a complex microbial assemblage.

Autor: Rashel V Grindberg, Thomas Ishoey, Dumitru Brinza, Eduardo Esquenazi, R Cameron Coates, Wei-ting Liu, Lena Gerwick, Pieter C Dorrestein, Pavel Pevzner, Roger Lasken, William H Gerwick

Publikováno v: PLoS ONE, Vol 6, Iss 4, p e18565 (2011)

Filamentous marine cyanobacteria are extraordinarily rich sources of structurally novel, biomedically relevant natural products. To understand their biosynthetic origins as well as produce increased supplies and analog molecules, access to the cluste

Externí odkaz: https://doaj.org/article/ab750730540e4b89a80c5d1e6195d2d8

Minimum Information about a Biosynthetic Gene cluster

Autor: Patrick Caffrey, Renzo Kottmann, Eriko Takano, Sean Doyle, Axel A. Brakhage, Matthew Cummings, Juan Pablo Gomez-Escribano, Yvonne Mast, Ryan F. Seipke, Rob Lavigne, Markus Nett, Hans-Wilhelm Nützmann, Jan Claesen, David H. Sherman, Daniel Petras, Pablo Cruz-Morales, Carl J. Balibar, Anne Osbourn, Oscar P. Kuipers, Leonilde M. Moreira, Xinyu Liu, Marcia S. Osburne, Bohdan Ostash, David P. Fewer, Changsheng Zhang, Pelin Yilmaz, Mohamed S. Donia, Anja Greule, Hyun Uk Kim, Nicholas J. Tobias, Frank Oliver Glöckner, Christoph Geiger, Chia Y. Lee, William H. Gerwick, Philipp Wiemann, Bertolt Gust, Susan E. Jensen, Wilfred A. van der Donk, Jan Kormanec, Ben Shen, Christopher M. Thomas, Jason Micklefield, Srikanth Duddela, R. Cameron Coates, René De Mot, Anthony S. Haines, Neha Garg, Guohui Pan, Roderich D. Süssmuth, Hyung Jin Kwon, Jonathan D. Walton, Lena Gerwick, Jörn Piel, Monika Ehling-Schulz, Zhenhua Tian, Jonathan L. Klassen, Xiaohui Yan, Emily A. Monroe, Yunchang Xie, Russell J. Cox, Keishi Ishida, Grace Yim, Stefano Donadio, Nadine Ziemert, Yuta Tsunematsu, Matthew L. Hillwig, Miroslav Petricek, Sylvie Lautru, Tilmann Weber, Andrew W. Truman, Rainer Breitling, Peter Kötter, Nikos C. Kyrpides, Stephanie Düsterhus, Christian Hertweck, Hideaki Oikawa, Sean F. Brady, Christopher T. Walsh, Adam C. Jones, Marcus A. Moore, Bradley S. Moore, Barrie Wilkinson, Simone M. Mantovani, Nathan A. Moss, Elizabeth E. Wyckoff, Emily P. Balskus, Kapil Tahlan, Fengan Yu, Monica Höfte, Jos M. Raaijmakers, Taifo Mahmud, Yit-Heng Chooi, Yi Tang, Andreas Bechthold, Douglas A. Mitchell, Joanne M. Willey, Helge B. Bode, John B. Biggins, Margherita Sosio, Yi-Qiang Cheng, Carmen Méndez, Leonard Kaysser, Joleen Masschelein, Daniel Krug, Federico Rosconi, Marnix H. Medema, Kaarina Sivonen, Tomohisa Kuzuyama, Mikko Metsä-Ketelä, Esther K. Schmitt, Carsten Kegler, Andriy Luzhetskyy, Gilles P. van Wezel, Bai Linquan, Kai Blin, Jens Nielsen, Bertrand Aigle, Amrita Pati, Harald Gross, Muriel Viaud, Pieter C. Dorrestein, Carla S. Jones, Michael A. Fischbach, Shelley M. Payne, Zhe Rui, Gerard D. Wright, Wen Liu, Alexey V. Melnik, Barry Scott, Brett A. Neilan, Nancy P. Keller, Rainer Borriss, Katrin Jungmann, Michalis Hadjithomas, Evi Stegmann, Daniel J. Edwards, F. Jerry Reen, Alexander Kristian Apel, Wolfgang Wohlleben, Michael J. Smanski, Leonard Katz, Fergal O'Gara, Eric J. N. Helfrich, Sergey B. Zotchev, Olivier Ploux, Arnold J. M. Driessen, Rolf Müller, Jean-Luc Pernodet, K. D. Entian, José A. Salas, Irene de Bruijn, Francisco Barona-Gómez, Jianhua Ju, Jon Clardy

Publikováno v: Nature Chemical Biology, 11(9), 625-631. Nature Publishing Group
Nature Chemical Biology 11 (2015), Nr. 9
Medema, M H, Kottmann, R, Yilmaz, P, Cummings, M, Biggins, J B, Blin, K, Bruijn, I D, Chooi, Y H, Claesen, J, Coates, R C, Cruz-Morales, P, Duddela, S, Düsterhus, S, Edwards, D J, Fewer, D P, Garg, N, Geiger, C, Gomez-Escribano, J P, Greule, A, Hadjithomas, M, Haines, A S, Helfrich, E J N, Hillwig, M L, Ishida, K, Jones, A C, Jones, C S, Jungmann, K, Kegler, C, Kim, H U, Kötter, P, Krug, D, Masschelein, J, Melnik, A V, Mantovani, S M, Monroe, E A, Moore, M, Moss, N, Nützmann, H-W, Pan, G, Pati, A, Petras, D, Reen, F J, Rosconi, F, Rui, Z, Tian, Z, Tobias, N J, Tsunematsu, Y, Wiemann, P, Wyckoff, E, Yan, X, Yim, G, Yu, F, Xie, Y, Aigle, B, Apel, A K, Balibar, C J, Balskus, E P, Barona-Gómez, F, Bechthold, A, Bode, H B, Borriss, R, Brady, S F, Brakhage, A A, Caffrey, P, Cheng, Y-Q, Clardy, J, Cox, R J, Mot, R D, Donadio, S, Donia, M S, Donk, W A V D, Dorrestein, P C, Doyle, S, Driessen, A J M, Ehling-Schulz, M, Entian, K-D, Fischbach, M A, Gerwick, L, Gerwick, W H, Gross, H, Gust, B, Hertweck, C, Höfte, M, Jensen, S E, Ju, J, Katz, L, Kaysser, L, Klassen, J L, Keller, N P, Kormanec, J, Kuipers, O P, Kuzuyama, T, Kyrpides, N C, Kwon, H-J, Lautru, S, Lavigne, R, Lee, C Y, Linquan, B, Liu, X, Liu, W, Luzhetskyy, A, Mahmud, T, Mast, Y, Méndez, C, Metsä-Ketelä, M, Micklefield, J, Mitchell, D A, Moore, B S, Moreira, L M, Müller, R, Neilan, B A, Nett, M, Nielsen, J, O'Gara, F, Oikawa, H, Osbourn, A, Osburne, M S, Ostash, B, Payne, S M, Pernodet, J-L, Petricek, M, Piel, J, Ploux, O, Raaijmakers, J M, Salas, J A, Schmitt, E K, Scott, B, Seipke, R F, Shen, B, Sherman, D H, Sivonen, K, Smanski, M J, Sosio, M, Stegmann, E, Süssmuth, R D, Tahlan, K, Thomas, C M, Tang, Y, Truman, A W, Viaud, M, Walton, J D, Walsh, C T, Weber, T, Wezel, G P V, Wilkinson, B, Willey, J M, Wohlleben, W, Wright, G D, Ziemert, N, Zhang, C, Zotchev, S B, Breitling, R, Takano, E & Glöckner, F O 2015, ' Minimum Information about a Biosynthetic Gene cluster ', Nature chemical biology, vol. 11, pp. 625-631 . https://doi.org/10.1038/nchembio.1890
Medema, M H, Kottmann, R, Yilmaz, P, Cummings, M, Biggins, J B, Blin, K, de Bruijn, I, Kim, H U, Nielsen, J & Weber, T 2015, ' Minimum Information about a Biosynthetic Gene cluster ', Nature Chemical Biology, vol. 11, no. 9, pp. 625-631 . https://doi.org/10.1038/nchembio.1890
ResearcherID
Nature Chemical Biology
Nature Chemical Biology, 2015, 11 (9), pp.625-31. ⟨10.1038/nchembio.1890⟩
Nature Chemical Biology, Nature Publishing Group, 2015, 11 (9), pp.625-31. ⟨10.1038/nchembio.1890⟩
Nature Chemical Biology, 11(9), 625-631
Nature Chemical Biology 11 (2015) 9
Scopus
Medema, M H, Kottmann, R, Yilmaz, P, Cummings, M, Biggins, J B, Blin, K, Bruijn, I D, Chooi, Y H, Claesen, J, Coates, R C, Cruz-Morales, P, Duddela, S, Düsterhus, S, Edwards, D J, Fewer, D P, Garg, N, Geiger, C, Gomez-Escribano, J P, Greule, A, Hadjithomas, M, Haines, A S, Helfrich, E J N, Hillwig, M L, Ishida, K, Jones, A C, Jones, C S, Jungmann, K, Kegler, C, Kim, H U, Kötter, P, Krug, D, Masschelein, J, Melnik, A V, Mantovani, S M, Monroe, E A, Moore, M, Moss, N, Nützmann, H-W, Pan, G, Pati, A, Petras, D, Reen, F J, Rosconi, F, Rui, Z, Tian, Z, Tobias, N J, Tsunematsu, Y, Wiemann, P, Wyckoff, E, Yan, X, Yim, G, Yu, F, Xie, Y, Aigle, B, Apel, A K, Balibar, C J, Balskus, E P, Barona-Gómez, F, Bechthold, A, Bode, H B, Borriss, R, Brady, S F, Brakhage, A A, Caffrey, P, Cheng, Y-Q, Clardy, J, Cox, R J, Mot, R D, Donadio, S, Donia, M S, Donk, W A V D, Dorrestein, P C, Doyle, S, Driessen, A J M, Ehling-Schulz, M, Entian, K-D, Fischbach, M A, Gerwick, L, Gerwick, W H, Gross, H, Gust, B, Hertweck, C, Höfte, M, Jensen, S E, Ju, J, Katz, L, Kaysser, L, Klassen, J L, Keller, N P, Kormanec, J, Kuipers, O P, Kuzuyama, T, Kyrpides, N C, Kwon, H-J, Lautru, S, Lavigne, R, Lee, C Y, Linquan, B, Liu, X, Liu, W, Luzhetskyy, A, Mahmud, T, Mast, Y, Méndez, C, Metsä-Ketelä, M, Micklefield, J, Mitchell, D A, Moore, B S, Moreira, L M, Müller, R, Neilan, B A, Nett, M, Nielsen, J, O'Gara, F, Oikawa, H, Osbourn, A, Osburne, M S, Ostash, B, Payne, S M, Pernodet, J-L, Petricek, M, Piel, J, Ploux, O, Raaijmakers, J M, Salas, J A, Schmitt, E K, Scott, B, Seipke, R F, Shen, B, Sherman, D H, Sivonen, K, Smanski, M J, Sosio, M, Stegmann, E, Süssmuth, R D, Tahlan, K, Thomas, C M, Tang, Y, Truman, A W, Viaud, M, Walton, J D, Walsh, C T, Weber, T, Wezel, G P V, Wilkinson, B, Willey, J M, Wohlleben, W, Wright, G D, Ziemert, N, Zhang, C, Zotchev, S B, Breitling, R, Takano, E & Glöckner, F O 2015, ' Minimum Information about a Biosynthetic Gene cluster ' Nature chemical biology, vol. 11, pp. 625-631 . https://doi.org/10.1038/nchembio.1890
Nature Chemical Biology, Nature Publishing Group, 2015, 11 (9), pp.625-31. 〈10.1038/nchembio.1890〉
RUO. Repositorio Institucional de la Universidad de Oviedo
instname

M.H.M. was supported by a Rubicon fellowship of the Netherlands Organization for Scientific Research (NWO;Rubicon 825.13.001). The work of R.K. was supported by the European Union’s Seventh Framework Programme(Joint Call OCEAN.2011–2: Marine micr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0d1aa4fd11e7fd63b1669a0f6239bbd
https://doi.org/10.1038/nchembio.1890

16S rRNA GENE HETEROGENEITY IN THE FILAMENTOUS MARINE CYANOBACTERIAL GENUS LYNGBYA1

Autor: William H. Gerwick, R. Cameron Coates, Niclas Engene

Publikováno v: Journal of Phycology. 46:591-601

The SSU (16S) rRNA gene was used to investigate the phylogeny of the cyanobacterial genus Lyngbya as well as examined for its capacity to discriminate between different marine species of Lyngbya .W e show that Lyngbya forms a polyphyletic genus compo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::32a8e5bacfc0147231c60b4e8e9b635f
https://doi.org/10.1111/j.1529-8817.2010.00840.x

Characterization of cyanobacterial hydrocarbon composition and distribution of biosynthetic pathways

Autor: Sheila Podell, Anton Korobeynikov, Alla Lapidus, William H. Gerwick, R. Cameron Coates, Pavel A. Pevzner, Eric E. Allen, David H. Sherman, Lena Gerwick

Publikováno v: PLoS ONE, Vol 9, Iss 1, p e85140 (2014)
PLoS ONE
PloS one, vol 9, iss 1
Coates, RC; Podell, S; Korobeynikov, A; Lapidus, A; Pevzner, P; Sherman, DH; et al.(2014). Characterization of cyanobacterial hydrocarbon composition and distribution of biosynthetic pathways. PLoS ONE, 9(1). doi: 10.1371/journal.pone.0085140. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/4th951q6

Cyanobacteria possess the unique capacity to naturally produce hydrocarbons from fatty acids. Hydrocarbon compositions of thirty-two strains of cyanobacteria were characterized to reveal novel structural features and insights into hydrocarbon biosynt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::453b4f83038e510d2c5b48c585443c70
http://europepmc.org/articles/PMC3903477?pdf=render