Zobrazeno 1 - 10
of 12
pro vyhledávání: '"R. Andrew Marshall"'
Autor:
Albert Tsai, Sotaro Uemura, Magnus Johansson, Elisabetta Viani Puglisi, R. Andrew Marshall, Colin Echeverría Aitken, Jonas Korlach, Måns Ehrenberg, Joseph D. Puglisi
Publikováno v:
Cell Reports, Vol 3, Iss 2, Pp 497-508 (2013)
Inferring antibiotic mechanisms on translation through static structures has been challenging, as biological systems are highly dynamic. Dynamic single-molecule methods are also limited to few simultaneously measurable parameters. We have circumvente
Externí odkaz:
https://doaj.org/article/fd8bba3747bd498782d3adb8bc8bff50
Publikováno v:
Biophysical Journal. 94:1826-1835
The application of single-molecule fluorescence techniques to complex biological systems places demands on the performance of single fluorophores. We present an enzymatic oxygen scavenging system for improved dye stability in single-molecule experime
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55a347ebfad0bf5ddbdf6d1706fbd23c
https://doi.org/10.1529/biophysj.107.117689
https://doi.org/10.1529/biophysj.107.117689
Autor:
Elisabetta Viani Puglisi, R. Andrew Marshall, Darrin A. Lindhout, Sean A. McKenna, Insil Kim, Colin Echeverría Aitken, Joseph D. Puglisi
Publikováno v:
Nature Protocols. 2:3270-3277
RNA synthesis using in vitro transcription by phage T7 RNA polymerase allows preparation of milligram quantities of RNA for biochemical, biophysical and structural investigations. Previous purification approaches relied on gel electrophoretic or grav
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::249d491dd16797d3fe978d76f6103e61
https://doi.org/10.1038/nprot.2007.480
https://doi.org/10.1038/nprot.2007.480
Autor:
Joseph D. Puglisi, R. Andrew Marshall
Publikováno v:
Aminoglycoside Antibiotics: From Chemical Biology to Drug Discovery
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4459fa3bed2d11cf487f751f65b18edb
https://doi.org/10.1002/9780470149676.ch5
https://doi.org/10.1002/9780470149676.ch5
Autor:
Jaroslav Zajicek, R. Andrew Marshall, Bradley D. Smith, Alicia M. Beatty, Joseph M. Mahoney, Rameshwer Shukla
Publikováno v:
The Journal of Organic Chemistry. 67:1436-1440
A crown ether-containing macrobicycle was used as the wheel component in a templated synthesis of a [2]rotaxane with an acetal-containing axle. The molecular structures of the macrobicycle and the [2]rotaxane were characterized by NMR spectroscopy an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f1c07004bae17679cfba051f7518ef5
https://doi.org/10.1021/jo0162787
https://doi.org/10.1021/jo0162787
Autor:
R. Andrew Marshall, Bradley D. Smith, Alicia M. Beatty, Philip A. Gale, Salvatore Camiolo, Joseph M. Mahoney
Publikováno v:
Journal of Supramolecular Chemistry. 1:289-292
A 2,5-diamidopyrrole-capped macrobicycle was synthesized and its chloride salt binding ability evaluated in polar and apolar organic solvents. X-ray crystal structures of the receptor and its sodium chloride complex were obtained and in the latter st
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7bb74c043347d7a1c99f0d11646d5018
https://doi.org/10.1016/s1472-7862(02)00068-0
https://doi.org/10.1016/s1472-7862(02)00068-0
Autor:
Joseph D. Puglisi, Alexey Petrov, Albert Tsai, Jonas Korlach, Sotaro Uemura, R. Andrew Marshall
Publikováno v:
Nature. 487(7407)
A single-molecule approach is used to investigate the kinetics of assembly of the translation initiation complex, revealing that there is more than one pathway by which the necessary factors can assemble. Translation initiation involves the associati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6c520655ed08341ae10db6123af721e
https://pubmed.ncbi.nlm.nih.gov/23019691
https://pubmed.ncbi.nlm.nih.gov/23019691
Recent structural data have revealed two distinct conformations of the ribosome during initiation. We employed single-molecule fluorescence methods to probe the dynamic relation of these ribosomal conformations in real time. In the absence of initiat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a967bfb1f08254e9b043f7d7bb18ba4
https://europepmc.org/articles/PMC4447082/
https://europepmc.org/articles/PMC4447082/
Publikováno v:
NATO Science for Peace and Security Series B: Physics and Biophysics ISBN: 9789048123674
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::803b6dca63b7337a6aed68075ac8e215
https://doi.org/10.1007/978-90-481-2368-1_6
https://doi.org/10.1007/978-90-481-2368-1_6
This chapter presents an overview of the single molecule methods that have been used for investigating translation and discusses the key discoveries from these methods on the dynamic nature of protein biosynthesis. Single molecule techniques, includi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::20be0817ae26b1219e25907915daaca2
https://doi.org/10.1016/b978-0-12-374227-8.00007-9
https://doi.org/10.1016/b978-0-12-374227-8.00007-9