Nuclear Run-On Assay Using Biotin Labeling, Magnetic Bead Capture and Analysis by Fluorescence-Based RT-PCR

Autor: G. Patrone, F. Puppo, R. Cusano, M. Scaranari, I. Ceccherini, A. Puliti, R. Ravazzolo

Publikováno v: BioTechniques, Vol 29, Iss 5, Pp 1012-1017 (2000)

In this report, we present a fluorescencebased approach to the assessment of cellular gene expression and transcription rates. Nuclear run-on was performed by supplying biotin-16-UTP to nuclei, and labeled transcripts were bound to streptavidin-coate

Externí odkaz: https://doaj.org/article/0eaa72cb9f724868a3c6f5638f18d3cd

DNSSEC in the ccTLD .it - Main EPP implementation choices

Autor: M. Loffredo, M. Martinelli, R. Ravazzolo

Publikováno v: IIT TR-01/2017, 2017

The DNS protocol (Domain Name System) defines the specifications to provide a domain name resolution service that has no form of authentication nor implements mechanisms to ensure data integrity. In order to overcome this limitation, the IETF has def

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=cnr_________::b36638f883f7fc46d71aab13ba1c2e5b
https://publications.cnr.it/doc/365435

Contents Vol. 92, 2001

Autor: L. Iannuzzi, C. Broccardo, Y. Mao, J.J.M. van Groningen, W.D. Phillips, J. Wang, J. Rathjen, T. Krieg, D.S. Gallagher, F. Sangiuolo, P. Lichter, M. Wilbrink, P.D. Rathjen, N.W. Richards, K.-M. Debatin, Y. Matsuda, A. Vaiman, K.J. Portbury, P. Perelman, F. Bourgeois, X. Huang, D.L. Gumucio, I. Jentsch, S.S. Bhattacharya, Q. Jiang, K. Ying, I. Matera, A. Rösen-Wolff, C. Mas, K. Ogura, J. Koch, S. Kiuru-Kuhlefelt, C. Auffray, P. Denèfle, R. Allikmets, M. Miettinen, S. Schmutz, M.P. Hildebrand, I. Arnould, C. Prades, R. Sauer, C. Sapienza, R.B. Voyle, F. Yang, M. Gahr, L.J. Coignet, A. Kuroiwa, C.G. Scheuerpflug, N. Serdukova, W.O. Bauer, L.M. Gulluyan, B. Brewer, M. Simonneau, J. Fanburg-Smith, A.S. Graphodatsky, I. Kola, C. Stover, M. Sancandi, J. Dai, W. El-Rifai, Y. Obara, M. Bozzali, M.-D. Devignes, E. Reichenberger, A.G. Marneros, T. Otoguro, B.R. Olsen, A. Botta, C. Drögemüller, A. Mincheva, W. Schwaeble, W. Wang, F. Jakob, S-I. Kawada, L. Naudin, B. Jordan, T. Isobe, M.R. Speicher, G. Palumbo, L. Granjon, S. Knuutila, C. Chelala, A. Bulfone, K. Engel, A. Hoffmann, E. Kater-Baats, H. Kuiper, R. Ravazzolo, G. Gradl, S. Fojo, Z. Zhou, M. Schmid, N. Serakıncı, D. Di Berardino, J. Rubes, I. Ceccherini, K. Matsumoto, B. Dallapiccola, K. Tsuchiya, J.M. Moalic, M.A. Lee-Kirsch, S. Galiègue-Zouitina, G. Merkx, S. Thomas, M.A.J. Weterman, V.T. Volobouev, M. Dean, M. Stumm, G. Novelli, F. Guimiot, N. Tommerup, M. Fredholm, A. Baldini, V. Jurecic, N.B. Atkin, W. Zhao, C. Hansen, W. Nie, G.P. Di Meo, I. Gustavsson, M. van Rooijen, S. Cirera, A.T. Remaley, J. Osorio, S. Imbeaud, R. Cinti, F. Amati, R.-D. Wegner, E. Conti, S. Murray, A. Geurts van Kessel, A. Pizzuti, C.M. Owczarek, K. Friis Henriksen, S. Halford, C. Lafargue, R. Tang, C.Y. Gregory-Evans, S. Cui, C.P. Popescu, P.J. Hertzog, T. Leeb, T. Ogura, P.E. Schaner, M. Kuro-o, Diederik R.H. de Bruijn, S. Shulenin, P. Wieacker, W. Rens, N.A. Jenkins, B. Pedersen, M. Fava, H.R. Treutlein, C. Roumier, M. Berloco, F. Pardo-Manuel de Villena, M. Rosier, P.C.M. O’Brien, N.D. Ebenezer, L. Kutschke, D. Fukushi, E.P. Cribiu, A. Ratti, G. Beck, O. Distl, M.-F. Luciani, S.E. Antonarakis, M. Eleveld, Y. Huang, R.M. Hope, J. Kere, J.A. Kennell, Y. Xie, B.S. Milne, M.A. Ferguson-Smith, S. Neubauer, J. Womack, R. Zoorob, H. Hayes, A. Eggen, N.G. Copeland, B. Levacher, Y.-A. Lee, B. Sicard, M. Bak, S.X. Liang, D.J. Gilbert, G. Stranzinger, E. Paditz, H. Mehenni, R. Wieser, G. Chimini, M.G. Mattei, L.M. Schriml, S. Keindorff, I. Nanda, M. Shichiri

Publikováno v: Cytogenetic and Genome Research. 92:363-365

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::00b283e01bc597e4d07a3a731ac04e44
https://doi.org/10.1159/000056936

A fibroblast-specific factor binds to an upstream negative control element in the promoter of the mouse alpha 1(I) collagen gene

Autor: R Ravazzolo, B de Crombrugghe, Gerard Karsenty

Publikováno v: Journal of Biological Chemistry. 266:7382-7387

We have identified, in nuclear extracts of NIH-3T3 fibroblasts, a factor which binds a sequence between -339 and -361 in the mouse alpha 1(I) collagen promoter. A 3-base pair substitution mutation introduced in this promoter element, which abolishes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c02854a37af881ff7f844b2d7675c6d0
https://doi.org/10.1016/s0021-9258(20)89458-7

Adenovirus E1A down-regulates the EGF receptor via repression of its promoter

Autor: M, Prudenziati, M, Sirito, H, van Dam, R, Ravazzolo

Publikováno v: International journal of cancer. 88(6)

The epidermal growth factor receptor (EGF-R), after activation by its ligands, stimulates a cascade of intracellular events leading to cellular proliferation. Its expression is increased in various forms of cancer as a consequence of altered regulati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8e5b34d9d5deebfb26f3af4b13f06835
https://pubmed.ncbi.nlm.nih.gov/11093819