Zobrazeno 1 - 10 of 11 pro vyhledávání: '"R W, Rubin"'
1
Direct biochemical measurements of microtubule assembly and disassembly in Chinese hamster ovary cells. The effect of intercellular contact, cold, D2O, and N6,O2'-dibutyryl cyclic adenosine monophosphate
Autor: G D Weiss, R W Rubin
Publikováno v: The Journal of Cell Biology
A study was undertaken to develop a means of quantitating the amount of tubulin present as a soluble pool and as intact microtubules in cultured Chinese hamster ovary cells. A procedure was developed in which these cells grown on monolayer culture in
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f56992b62674b399549cd24559b4d5d1
https://doi.org/10.1083/jcb.64.1.42
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2
Comparison of the protein content of three different bovine secretory granule membrane types: a search for exocytosis-specific shared proteins
Autor: R W Rubin, B C Pressman, A K Lyubkin
Publikováno v: The Journal of Cell Biology
A two-dimensional polyacrylamide gel analysis of three types of bovine exocytotic granule membranes has been undertaken. Great care was taken to purify the membranes of biochemical homogeneity with minimal contamination from other membrane sources. T
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::797da4fb3277946c18beb561ffb3b403
https://doi.org/10.1083/jcb.99.1.356
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3
Isolation and electrophoretic analysis of nucleoli, phenol-soluble nuclear proteins, and outer cyst walls from Acanthamoeba castellanii during encystation initiation
Autor: J Boehmer, R W Rubin, M C Hill, P Hepworth
Publikováno v: The Journal of Cell Biology
A technique is described for isolating nuceoli from Acanthamoeba castellanii. Nuclei isolated by a modification of the technique of F. J. Chlapowski and R. N. Band (1971) are sonicated in a surcrose-Tris-MgSO4-KC1-Triton X-100 buffer and centrifuged
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29c7dda5bbe37811f66b746870a85082
https://doi.org/10.1083/jcb.68.3.740
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4
Subcellular fractions of the adrenal medulla. Comparison by two-dimensional polyacrylamide gel electrophoresis
Autor: J J, Corcoran, F A, Lattanzio, R W, Rubin, B C, Pressman
Publikováno v: Biochimica et biophysica acta. 707(2)
Subfractions of adrenal medullary homogenates were analyzed in two-dimensional polyacrylamide gels to assess the extent of protein homology. Chromaffin granule proteins were highly acidic, with the exception of the soluble form of the enzyme dopamine
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c7909a367e358bc615428a91df8ea4c1
https://pubmed.ncbi.nlm.nih.gov/7138885
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5
Cyclic changes in the cell surface. I. Change in thymidine transport and its inhibition by cytochalasin B in Chinese hamster ovary cells
Autor: L P, Everhart, R W, Rubin
Publikováno v: The Journal of Cell Biology
Cytochalasin B (CB) shows a marked concentration-dependent inhibition of the incorporation of [(3)H]thymidine into Chinese hamster ovary cells. This inhibition was shown to result from an inhibition of thymidine uptake, not from an inhibition of DNA
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6319a84e9eac47c5040f96ab7c0b54ec
https://pubmed.ncbi.nlm.nih.gov/4855903
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6
Cyclic changes in the cell surface. II. The effect of cytochalasin B on the surface morphology of synchronized Chinese hamster ovary cells
Autor: L P, Everhart, R W, Rubin
Publikováno v: The Journal of Cell Biology
The surface morphology of Chinese hamster ovary cells treated with cytochalasin B (CB) has been examined using the scanning electron microscope. The cells respond to treatment with CB by retracting peripheral processes, rounding up, and assuming a sm
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::646c3d0bb34243f8eeeb7d8108a63a79
https://pubmed.ncbi.nlm.nih.gov/4855904
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9
Tubulin as a major cell surface protein in human lymphoid cells of leukemic origin
Autor: R W, Rubin, M, Quillen, J J, Corcoran, R, Ganapathi, A, Krishan
Publikováno v: Cancer research. 42(4)
Surface-exposed proteins of vinblastine-sensitive human lymphoid cell line of leukemic origin (CCRF-CEM) were examined by the lactoperoxidase-catalyzed iodination and two-dimensional polyacrylamide gel electrophoresis methods. Spots which comigrate w
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::59b5317338c019a6b729bdaffc0be4bd
https://pubmed.ncbi.nlm.nih.gov/6949640
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10
Two-dimensional gel electrophoresis of membrane proteins from the R3327 prostate adenocarcinoma
Autor: P L, Kozlovskis, A J, Claflin, M A, Fletcher, E C, McKinney, R W, Rubin
Publikováno v: Cancer research. 42(7)
The Dunning rat prostate adenocarcinoma (R3327) is a reliable model that shares many similarities with the human tumor. Two sublines of the tumor, G and H, represent opposite extremes in histology and growth rate. Purified membrane fractions from G a
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::87de6d6d327103dcd4c3988023bc9e64
https://pubmed.ncbi.nlm.nih.gov/7083165
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