Zobrazeno 1 - 10
of 12
pro vyhledávání: '"R V, Guntaka"'
Autor:
R V, Guntaka, S K, Swamynathan
Publikováno v:
Indian journal of experimental biology. 36(6)
Although there are many hurdles to overcome for successful gene therapy, there is a vast potential to permanently incorporate genes into cells to correct genetic disorders and to combat viral infections. Retroviruses, inspite of some limitations, off
Publikováno v:
Folia biologica. 43(4)
The sequences in the LTR of Rous sarcoma virus that are required for transcription termination and polyadenylation have been determined. A vector containing LTR-neo-LTR has been constructed and deletions in the U5 region of the downstream LTR have be
Autor:
J C, Kandala, R V, Guntaka
Publikováno v:
Virology. 198(2)
A cDNA clone was isolated from a chicken embryo cDNA library employing a PCR-generated radiolabeled probe specific for the U3 region of the Rous sarcoma virus LTR. The cDNA encodes a protein of 345 aa and is homologous to the Y-box (inverted CCAAT) b
Publikováno v:
Folia biologica. 40(5)
Publikováno v:
BioTechniques. 13(3)
The ability to successfully screen a lambda gt11 cDNA expression library for specific gene products that can bind to selected sequences of DNA depends on radioactive double-stranded DNA probes with high specific activity. We demonstrate here that pro
Publikováno v:
Journal of General Virology. 64:1885-1893
Summary The structure and arrangement of the multiple provirus copies of avian sarcoma virus in a rat XC cell line were studied by restriction endonucleases. The following observations were made: (i) the majority of the proviruses integrated randomly
Publikováno v:
Scopus-Elsevier
The nucleotide sequences in the long terminal repeat of avian sarcoma virus that are recognized in vitro by HeLa cell RNA polymerase II have been identified. For this purpose, various 5' and 3' deletions were introduced into a cloned long terminal re
Publikováno v:
Molecular and Cellular Biology. 3:1975-1984
The regulatory elements present in the long terminal repeat (LTR) of avian sarcoma virus DNA were analyzed by recombinant DNA techniques coupled with DNA-mediated gene transfer in avian as well as mammalian cells. For this purpose, the neomycin resis
Publikováno v:
Gene. 16:217-225
A sequence in the long terminal repeat (LTR) of avian tumor virus (ATV) DNA was shown to contain a promoter active in Escherichia coli . For this analysis the bacterial promoters for the tetracycline (Tc) and neomycin (Nm) resistance genes were delet
Publikováno v:
Journal of Virology. 28:1015-1017
Circular viral DNA intermediates obtained from the quail tumor line, QT6, at 1 day after infection, were opened at one specific location by the single-strand specific nuclease, S1, of Aspergillus oryzae. This site was no longer accessible to the S1 n