Zobrazeno 1 - 3
of 3
pro vyhledávání: '"R L Mullinax"'
Publikováno v:
BioTechniques. 12(6)
A new method is presented for creating antibody expression libraries in Escherichia coli. Rather than perform two cloning steps to express the heavy and light chains of the antigen binding domain, we have used a fusion-PCR method to link the coding r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::10492a2a3574742f9b1ed82928f796ca
https://pubmed.ncbi.nlm.nih.gov/1642894
https://pubmed.ncbi.nlm.nih.gov/1642894
Autor:
H H Hogrefe, M M Kubitz, M Alting-Mees, R L Mullinax, D Ardourel, B N Hay, E A Gross, J R Amberg, Jay M. Short, A Greener
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 87(20)
We have applied a molecular biology approach to the identification of human monoclonal antibodies. Human peripheral blood lymphocyte mRNA was converted to cDNA and a select subset was amplified by the polymerase chain reaction. These products, contai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b33755bccdb4ef4f868176ec37e5d2dd
https://pubmed.ncbi.nlm.nih.gov/2146680
https://pubmed.ncbi.nlm.nih.gov/2146680
Publikováno v:
Journal of neurogenetics. 4(6)
We have cloned the cDNA encoding human liver glycogen phosphorylase (glycogenosis type VI) from a fetal brain cDNA library. Liver(L) and muscle(M) phosphorylase cDNA probes were used to determine the relative abundance of mRNA encoding the L- and M-i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d0b151f250437606503cbffe9de58fde
https://pubmed.ncbi.nlm.nih.gov/3509980
https://pubmed.ncbi.nlm.nih.gov/3509980