Výsledky vyhledávání - "R L DeBiasio"

Cell-Based, High-Content Screen for Receptor Internalization, Recycling and Intracellular Trafficking

Autor: Bruce R. Conway, Y T Chen, Lisa Minor, Keith T. Demarest, R L DeBiasio, Richik N. Ghosh, Richard Debiasio

Publikováno v: BioTechniques. 29:170-175

A variety of physiologically important receptors are internalized and then recycled back to the plasma membrane by the endocytic recycling compartment. These include the transferrin receptor and many G-protein coupled receptors (GPCRs). The internali

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0ec1eddefd820db00350c5d65d6a27a
https://doi.org/10.2144/00291pf01

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High-Content Screening: A New Approach to Easing Key Bottlenecks in the Drug Discovery Process

Autor: D. Lansing Taylor, R L DeBiasio, Joseph M. Zock, Kenneth A. Giuliano, George N. Pavlakis, Albert Gough, Joanne M. Volosky, R. Terry Dunlay

Publikováno v: SLAS Discovery. 2:249-259

Recent improvements in target discovery and high throughput screening (HTS) have increased the pressure at key points along the drug discovery pipeline. High-content screening (HCS) was developed t...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8a844bd59dcf2ac0a9b1c66bb020504f
https://doi.org/10.1177/108705719700200410

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Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD)

Autor: Zhengrong Zhu, Brigitte F. Schmidt, Jean Chao, Kenneth A. Giuliano, R L DeBiasio

Publikováno v: Cytometry. 23:48-53

Progress has been made in improving the immunohistochemical detection of antigens for imaging and flow cytometry. We report the synthesis of a novel fluorescent horseradish peroxidase substrate, Cy3.29-tyramide, and its application in an enzyme-based

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6bbf98eed5a45f9e9ccd7d79731e2a51
https://doi.org/10.1002/(sici)1097-0320(19960101)23:1<48::aid-cyto7>3.0.co

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A fluorescent protein biosensor of myosin II regulatory light chain phosphorylation reports a gradient of phosphorylated myosin II in migrating cells

Autor: P L Post, R L DeBiasio, D L Taylor

Publikováno v: Molecular Biology of the Cell. 6:1755-1768

Phosphorylation of the regulatory light chain by myosin light chain kinase (MLCK) regulates the motor activity of smooth muscle and nonmuscle myosin II. We have designed reagents to detect this phosphorylation event in living cells. A new fluorescent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f37b2d3f844ce5cbd81a853c4fd08c3
https://doi.org/10.1091/mbc.6.12.1755

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Antigen Presentation and Cytotoxic T Lymphocyte Killing Studied in Individual, Living Cells

Autor: D. Lansing Taylor, R L DeBiasio, Antoinette Tishon, Michael B. A. Oldstone, Hanna Lewicki, Greg LaRocca, Jean Edouard Gairin, Klaus M. Hahn

Publikováno v: Virology. 201:330-340

Interactions between individual, living fibroblasts and cytotoxic T lymphocyte (CTL) clones were analyzed by using video-enhanced differential interference contrast and fluorescence microscopy in a multimode configuration. Fibroblasts expressing know

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd53581280d51d9ae89c1713140f576d
https://doi.org/10.1006/viro.1994.1298

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Potential of Machine-Vision Light Microscopy in Toxicologic Pathology

Autor: Alan S. Waggoner, D. Pane, Bert Gough, Daniel L. Farkas, Judy Yu, John Kolega, Alasdair I. Dow, Kevin Burton, Ken Giuliano, Greg LaRocca, R L DeBiasio, D. Lansing Taylor, Penny Post

Publikováno v: Toxicologic Pathology. 22:145-159

Major developments in machine-vision light microscopy and in reagent chemistry have led to a renaissance and revolution in the use of the light microscope in biology, biotechnology, and medicine. The potential use of this technology in the field of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b4a5da3effe5c6c6e61b7240facd96f
https://doi.org/10.1177/019262339402200208

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Multimode Light Microscopy and the Dynamics of Molecules, Cells, and Tissues

Autor: M A Nederlof, Kevin W. Ryan, Albert Gough, R L DeBiasio, D L Taylor, G Baxter, D R Patek, D Pane, Daniel L. Farkas, J Pane

Publikováno v: Scopus-Elsevier
Europe PubMed Central

There is a renaissance and revolution in light microscopy and its use in biological research, biotechnology, and clinical diagnostics (139, 163). This renaissance has been driven primarily by the need to define the interplay of ions, metabolites, and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d0b6607358ce81d6fbc065dd337759c
https://doi.org/10.1146/annurev.ph.55.030193.004033

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Patterns of elevated free calcium and calmodulin activation in living cells

Autor: Klaus M. Hahn, R L DeBiasio, D. Lansing Taylor

Publikováno v: Nature. 359:736-738

THE temporal and spatial dynamics of intracellular signals and protein effectors are being defined as a result of imaging using fluorescent reagents within living cells1–5. We have described a new class of fluorescent analogues2 termed optical bios

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fb7bbeffb16d79bdf92e15e20080122
https://doi.org/10.1038/359736a0

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