Zobrazeno 1 - 10
of 44
pro vyhledávání: '"R L Cysyk"'
Autor:
J R Kitchen, R L Cysyk
Publikováno v:
The Biochemical journal. 309
Hyaluronic acid (HA) and its synthesis were studied in intact Swiss 3T3 mouse fibroblasts and isolated membranes. HA chains in culture medium, attached to cells and in isolated membranes, were determined to possess average M(r) values of 5.2 x 10(6),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d6c14d3c9ce0d460e7bed500d860254
https://pubmed.ncbi.nlm.nih.gov/7626032
https://pubmed.ncbi.nlm.nih.gov/7626032
Publikováno v:
Biochemistry and molecular biology international. 35(5)
An improved assay for hyaluronic acid (HA) synthetase is described that is suitable for rapid processing of large numbers of samples. High background levels of unincorporated radioactivity are removed by passage of the reaction through a Sephadex G-5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::13673f1e422c7e84e3fb04f2a623753e
https://pubmed.ncbi.nlm.nih.gov/7549931
https://pubmed.ncbi.nlm.nih.gov/7549931
Publikováno v:
Biochemistry and molecular biology international. 31(4)
Vanadate (50 microM) increases the incorporation of 3H from D-[1,6-3H]glucosamine into hyaluronic acid (HA) in serum stimulated Swiss 3T3 fibroblasts. This increase is not a result of increased HA synthesis, but is due to a significant increase in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a092cd5e93db6f811681b3a28fb7dd8c
https://pubmed.ncbi.nlm.nih.gov/7507761
https://pubmed.ncbi.nlm.nih.gov/7507761
Publikováno v:
Biochemistry and molecular biology international. 30(5)
A new HPLC assay was developed to measure UDP-glucose dehydrogenase (UDP-GDH) activity in crude homogenates of 3T3 fibroblasts. UDP-GDH activity is directly related to the proliferative activity of the cell culture: enzyme activity is highest in log
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d76a09891a8181b04f43188bd7ca0ddb
https://pubmed.ncbi.nlm.nih.gov/8220234
https://pubmed.ncbi.nlm.nih.gov/8220234
Publikováno v:
Oncology research. 5(10-11)
Hyaluronic acid (HA) is an extracellular matrix glycosaminoglycan localized in the stroma of solid tumors, where it facilitates cell movement and thus tumor invasion and metastasis. This localization of HA is due to its synthesis by stromal fibroblas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2d81a4e77e28ce4f1b7d331558229411
https://pubmed.ncbi.nlm.nih.gov/8054702
https://pubmed.ncbi.nlm.nih.gov/8054702
Publikováno v:
Biochemistry international. 20(6)
Chromatographic analysis of the acid soluble nucleotide pool in 3T3 fibroblasts 30 minutes after serum stimulation revealed one component that was increased compared to quiescent controls. This component was identified as UDP-glucuronide by chromatog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0d385102609c4cf586228f44d8505a6b
https://pubmed.ncbi.nlm.nih.gov/2196057
https://pubmed.ncbi.nlm.nih.gov/2196057
Publikováno v:
Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry. 36:3012-3016
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::40cb39d0fcaf7c444411a2ebcf6c860b
https://doi.org/10.1107/s0567740880010709
https://doi.org/10.1107/s0567740880010709
Publikováno v:
Journal of Biological Chemistry. 258:13564-13569
The activity of the de novo pyrimidine biosynthetic pathway has been measured in resistant and sensitive murine tumors in vivo following a single intraperitoneal dose of N-phosphonacetyl-L-aspartate (PALA) (400 mg/kg). For these studies, we utilized
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c3a566967c9ec7f9fec7581f5098f182
https://doi.org/10.1016/s0021-9258(17)43951-2
https://doi.org/10.1016/s0021-9258(17)43951-2
Publikováno v:
Journal of Biological Chemistry. 259:67-72
The concentration of uridine in the media of cultured L1210 cells was maintained within the concentration range found in plasma (1 to 10 microM) to determine if such concentrations are sufficient to satisfy the pyrimidine requirements of a population
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ea9739e26d76aaa8d85b510be09165d6
https://doi.org/10.1016/s0021-9258(17)43622-2
https://doi.org/10.1016/s0021-9258(17)43622-2
Publikováno v:
Journal of Biological Chemistry. 260:4276-4281
A novel approach to the analysis of mass spectrally assayed stable isotope-labeling experiments for studies of biosynthetic pathways is reported. This method determines in a mixture of product molecules, the relative number of product molecules synth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8761eb3323f07af5aacef1fb36665744
https://doi.org/10.1016/s0021-9258(18)89260-2
https://doi.org/10.1016/s0021-9258(18)89260-2