Výsledky vyhledávání - "R K, Hickman"

Overproduction and purification of the Tn10-specified inner membrane tetracycline resistance protein Tet using fusions to ?-galactosidase

Autor: Stuart B. Levy, R K Hickman, Laura M. McMurry

Publikováno v: Molecular Microbiology. 4:1241-1251

Tetracycline resistance in the Enterobacteriaceae is mediated by a number of genetically related, usually plasmid-borne, determinants which specify an efflux system involving an inner membrane protein, Tet. Attempts to overproduce the Tn10 (Class B)-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96b7d5bcc8cdeadd8019ec62a44313e6
https://doi.org/10.1111/j.1365-2958.1990.tb00703.x

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Analysis of recombinant protein expression by MALDI-TOF mass spectrometry of bacterial colonies

Autor: M A, Winkler, R K, Hickman, A, Golden, H, Aboleneen

Publikováno v: BioTechniques. 28(5)

E. coli expressing soluble recombinant HIV antigens were analyzed directly by MALDI-TOF mass spectrometry (MS) from bacterial colonies picked from agar plates. An HIV envelope (ENV) antigen construct, penvA, was expressed in E. coli by transformation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fca0c1cd4eb07b1e5ff31d41552ac08e
https://pubmed.ncbi.nlm.nih.gov/10818694

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Molecular analyses of HIV-1 group O and HIV-2 variants from Africa

Autor: J C, Hunt, C A, Brennan, A M, Golden, J, Yamaguchi, J K, Lund, A S, Vallari, R K, Hickman, L, Zekeng, L G, Gürtler, H, Hampl, L, Kaptué, S G, Devare

Publikováno v: Leukemia. 11

Genetic variation among HIV isolates creates challenges for their detection by serologic and genetic techniques. To characterize the sequence variation and its correlation to serologic diversity of HIV-1 Group O and HIV-2 isolates, samples were ident

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::07b00195c6942f11dbfc4ebe70964eaa
https://pubmed.ncbi.nlm.nih.gov/9209322

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Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene

Autor: M S Curiale, R K Hickman, Stuart B. Levy, C Heuer, Wolfgang Hillen

The Tn10-like constitutively expressed tetracycline resistance determinant from a Haemophilus parainfluenzae strain was cloned in Escherichia coli. Toxicity resulting from expression on multicopy plasmids necessitated its being cloned on a low-copy p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e693cf2e45a74911e052ea79262b2e80
https://europepmc.org/articles/PMC211891/

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Evidence that TET protein functions as a multimer in the inner membrane of Escherichia coli

Autor: R K Hickman, Stuart B. Levy

Publikováno v: Journal of bacteriology. 170(4)

The inner membrane TET (TetA) protein, which is involved in Tn10-mediated microbial tetracycline resistance, consists of two domains, alpha and beta, both of which are needed for tetracycline resistance and efflux (M.S. Curiale, L.M. McMurry, and S.B

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e493c976ab575955a84b18f67fe9392
https://pubmed.ncbi.nlm.nih.gov/3280550

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Isolation of a stable cell wall-defective form of Neisseria gonorrhoeae from a case of untreated gonococcal urethritis

Autor: R K Hickman, J W Lawson

A cell wall-defective form of Neisseria gonorrhoeae from an exudate of an untreated patient with gonococcal urethritis was isolated on medium containing 7% polyvinylpyrrolidone. Initial attempts to grow the organism by standard microbiological method

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee2fd8be65786f4bd88972b9d31973d3
https://europepmc.org/articles/PMC273645/

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