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Epoetin alpha and beta in their erythropoetin isoform compositions and biological properties

Autor: R. J. Tiplady, Bridget E. Stenning, B. Rafferty, P. L. Storring, Lee J, Lamikanra A, Gaines Das Re

Publikováno v: British Journal of Haematology. 100:79-89

Epoetin alfa and beta are the two forms of recombinant DNA-derived erythropoietin (rEPO), both synthesized in Chinese hamster ovary cells, which are used for the treatment of erythropoietin (EPO)-responsive anaemias. Several batches of each of these

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::590962d305d9696faaa50dbddfc16f88
https://doi.org/10.1046/j.1365-2141.1998.00521.x

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Lectin-binding assays for the isoforms of human erythropoietin: comparison of urinary and four recombinant erythropoietins

Autor: Yogesh Mistry, R. E. Gaines Das, B. Rafferty, P. L. Storring, R. J. Tiplady

Publikováno v: Journal of Endocrinology. 150:401-412

Assays have been developed for the isoforms of erythropoietin (EPO) based on their binding to eight different lectins. These assays were used to compare the isoform compositions of two preparations of human urinary EPO (uEPO) and four preparations of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f842b22bf326bf7eb142c7dcf3e328c
https://doi.org/10.1677/joe.0.1500401

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Relationships between the N-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures

Autor: C T, Yuen, P L, Storring, R J, Tiplady, M, Izquierdo, R, Wait, C K, Gee, P, Gerson, P, Lloyd, J A, Cremata

Publikováno v: Advances in experimental medicine and biology. 564

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fcde46925dcfdda38f248eb5817d20a6
https://pubmed.ncbi.nlm.nih.gov/16400821

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Epoetin alfa and beta differ in their erythropoietin isoform compositions and biological properties

Autor: P L, Storring, R J, Tiplady, R E, Gaines Das, B E, Stenning, A, Lamikanra, B, Rafferty, J, Lee

Publikováno v: British journal of haematology. 100(1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f78ba153e87a06bee6377ab988fa710b
https://pubmed.ncbi.nlm.nih.gov/9450795

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A simple sensitive method, applicable to small polypeptides, for the determination of proteins in polyacrylamide gels after isoelectric focusing

Autor: R J Tiplady, P L Storring

Publikováno v: Biochemical Journal. 171:79-82

A simple method is described for the determination of polypeptides and proteins in polyacrylamide gels after isoelectric focusing. Precipitates formed in trichloroacetic acid, under controlled conditions, are quantified densitometrically by measuring

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b07a4b7fb5c0b91c336a9caa84be88c
https://doi.org/10.1042/bj1710079

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Stability of the Third International Standard for corticotrophin: accelerated degradation study using different bioassays and isoelectric focusing

Autor: R. J. Tiplady, Rose E. Gaines-Das, Bridget E. Stenning, P. L. Storring, Y. G. Mistry

Publikováno v: The Journal of endocrinology. 85(3)

The corticotrophin activity of ampoules of the Third International Standard for Corticotrophin (IS) kept at 20 and 37 °C for 15 years was compared with that of ampoules of the IS stored under normal conditions (−20 °C in the dark), using adrenal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c5b13ae747b5281bfa1583dcc3a135d
https://pubmed.ncbi.nlm.nih.gov/6251152

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