Zobrazeno 1 - 10
of 33
pro vyhledávání: '"R J Mattaliano"'
Autor:
Stewart Craig, G. Marius Clore, R J Mattaliano, Angela M. Gronenborn, Ursula Schmeissner, Paul T. Wingfield, H. Robson MacDonald
Publikováno v:
"Protein Engineering, Design and Selection". 1:413-417
Recombinant-derived human interleukin-1 alpha (IL-1 alpha), purified from Escherichia coli, was resolved by isoelectric focusing on polyacrylamide gels into two species of isoelectric points (pI) 5.45 and 5.20, which constituted approximately 75% and
Publikováno v:
Transfusion. 18:685-692
Granulocytes were isolated from the buffy coat of baboon blood by counterflow centrifugation. They were frozen in polypropylene tubes in 2.0 ml volumes containing 1 × 107 granulocytes. The medium consisted of 5% DMSO, 6% HES, 4% human serum albumin,
Publikováno v:
Proceedings of the National Academy of Sciences. 84:3992-3996
The genes that encode the α, β, and γ subunits of component C of m ethyl c oenzyme M r eductase ( mcrA, mcrB , and mcrG ) in Methanococcus vannielii have been cloned and sequenced, and their expression in Escherichia coli has been demonstrated. Th
Autor:
Lesley K. Sinclair, C Burne, E P Chow, Jeffrey L. Browning, R J Mattaliano, R B Pepinsky, G T Miller, D Pratt, Richard Tizard, K S Huang
Publikováno v:
Journal of Biological Chemistry. 263:10799-10811
We have purified two 35-kDa proteins from rat peritoneal lavages that inhibit phospholipase A2 activity. Both are calcium/phospholipid-dependent membrane binding proteins and share similar structural and biochemical properties with lipocortins I and
Publikováno v:
Journal of Molecular Biology. 203:971-983
A cDNA encoding DNA (cytosine-5)-methyltransferase (DNA MeTase) of mouse cells has been cloned and sequenced. The nucleotide sequence contains an open reading frame sufficient to encode a polypeptide of 1573 amino acid residues, which is close to the
Autor:
Patricia K. Donahoe, R J Mattaliano, Richard L. Cate, E P Chow, T F Manganaro, Lesley K. Sinclair, R B Pepinsky
Publikováno v:
Journal of Biological Chemistry. 263:18961-18964
Mullerian inhibiting substance (MIS) is a differentiation factor that causes the Mullerian duct to regress during the development of the male reproductive tract. The active form is a disulfide-linked dimer consisting of two identical 70-kDa subunits.
Publikováno v:
Journal of Biological Chemistry. 262:7639-7645
Human lipocortin I is a 38.5-kDa phospholipase A2 inhibitor that has been produced in Escherichia coli in large quantities by recombinant DNA technology (Wallner, B.P., Mattaliano, R.J., Hession, C., Cate, R. L., Tizard, R., Sinclair, L.K., Foeller,
Autor:
Kuo-Sen Huang, John C.-T. Tang, John E. Smart, C Burne, R. Blake Pepinsky, Richard Tizard, Barbara P. Wallner, E. Pingchang Chow, Paula McGray, Catherine Hession, Lesley K. Sinclair, Alexis Z. Frey, Jeffrey L. Browning, R J Mattaliano, K. L. Ramachandran
Publikováno v:
Cell. 46:191-199
We have purified two 35 kd phospholipase A2 inhibitors from human placenta, which we refer to as lipocortin I and II. Both proteins exhibit similar biochemical properties and occur in placenta at about 0.2% of the total protein. By peptide mapping, s
Publikováno v:
Biochemical Journal. 253:511-516
5-Ketofructose reductase [D(-)fructose:(NADP+) 5-oxidoreductase] was purified to homogeneity from Erwinia citreus and demonstrated to catalyse the reversible NADPH-dependent reduction of 5-ketofructose (D-threo-2,5-hexodiulose) to D-fructose. The enz
Autor:
K. L. Ramachandran, R J Mattaliano, E P Chow, Jürgen Brosius, Tomas Kirchhausen, John E. Smart, Stephen C. Harrison
Publikováno v:
Proceedings of the National Academy of Sciences. 84:8805-8809
We have deduced the 1675-amino acid sequence of rat clathrin heavy chain from cDNA clones and predict a protein of Mr 191,569. We have established the polarity of the heavy chain and assigned sequence positions to several structural landmarks of the