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pro vyhledávání: '"R J, Zagursky"'
Autor:
S. Haney, R. J. Zagursky, Ellen Murphy, Shang Wei Wu, E. Brown, Paul M. Dunman, D. Shlaes, D. Palacios, Steven J. Projan, G. Tucker-Kellogg
The advent of transcription profiling technologies has provided researchers with an unprecedented ability to study biological processes. Accordingly, a custom-made Affymetrix GeneChip, constituting >86% of the Staphylococcus aureus genome, was used t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53878652ea8fce730730a8f2a5761edb
https://europepmc.org/articles/PMC95583/
https://europepmc.org/articles/PMC95583/
Publikováno v:
Developments in biologicals. 103
Currently, there is an extensive and unprecedented effort to obtain the complete nucleotide sequence of the complex genomes of many micro-organisms. In this post-genomic era, based on the availability of the entire genome sequence of an organism, thr
Publikováno v:
BioTechniques. 18(3)
Flow cytometry provides a rapid qualitative method for analyzing the binding of a fluorescent probe to a cell. To quantitate the binding of a probe using flow cytometry, one must be able to first calibrate the fluorescent signal with some known stand
Publikováno v:
BioTechniques. 11(1)
The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [a
Autor:
N A, Straus, R J, Zagursky
Publikováno v:
BioTechniques. 10(3)
A method has been developed for the preparation of large single-stranded DNA sequencing templates from primary cloning plasmids or cosmids. The method utilizes the separate action of T7 Gene 6 exonuclease and exonuclease III to generate large quantit
Autor:
R J, Zagursky, R M, McCormick
Publikováno v:
BioTechniques. 9(1)
DNA sequencing separations of standard DNA fragments of known sequence have been achieved in small diameter capillary gels electrophoresed and analyzed in parallel in a modified commercial DNA sequencer instrument. DNA sequencing in terms of base-cal
Publikováno v:
Journal of bacteriology. 169(12)
We have combined techniques of genetic and physical mapping with rapid DNA sequence analysis to identify the nucleotide change in lambda int mutations. These mutations define two dominant phenotypic classes: (i) recombination that is partially indepe