Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance.

Autor: Deng X; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Achari A; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Federman S; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Yu G; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Somasekar S; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA., Bártolo I; Research Institute for Medicines, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal., Yagi S; Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA, USA., Mbala-Kingebeni P; Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo., Kapetshi J; Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo., Ahuka-Mundeke S; Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo., Muyembe-Tamfum JJ; Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo., Ahmed AA; Boston Children's Hospital, Boston, MA, USA.; Harvard Medical School, Boston, MA, USA., Ganesh V; Massachussetts General Hospital, Boston, MA, USA., Tamhankar M; Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA., Patterson JL; Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA., Ndembi N; Institute for Human Virology Nigeria, Abuja, Nigeria.; Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA., Mbanya D; Universite de Yaoundé I, Yaoundé, Cameroon.; University of Bamenda, Bamenda, Cameroon., Kaptue L; Université des Montagnes, Bangangté, Cameroon., McArthur C; University of Missouri-Kansas City, Kansas City, MO, USA., Muñoz-Medina JE; Instituto Mexicano del Seguro Social, Mexico City, Mexico., Gonzalez-Bonilla CR; Instituto Mexicano del Seguro Social, Mexico City, Mexico., López S; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico., Arias CF; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico., Arevalo S; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA., Miller S; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA., Stone M; Blood Systems Research Institute, San Francisco, CA, USA., Busch M; Blood Systems Research Institute, San Francisco, CA, USA., Hsieh K; Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA, USA., Messenger S; Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA, USA., Wadford DA; Viral and Rickettsial Disease Laboratory, California Department of Public Health, Richmond, CA, USA., Rodgers M; Abbott Laboratories, Abbott Park, IL, USA., Cloherty G; Abbott Laboratories, Abbott Park, IL, USA., Faria NR; Department of Zoology, University of Oxford, Oxford, UK., Thézé J; Department of Zoology, University of Oxford, Oxford, UK., Pybus OG; Department of Zoology, University of Oxford, Oxford, UK., Neto Z; Angolan National Institute of Health Research, Luanda, Angola., Morais J; Angolan National Institute of Health Research, Luanda, Angola., Taveira N; Research Institute for Medicines, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal.; Instituto Universitário Egas Moniz (IUEM), Monte de Caparica, Portugal., R Hackett J Jr; Abbott Laboratories, Abbott Park, IL, USA., Chiu CY; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA. charles.chiu@ucsf.edu.; UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA. charles.chiu@ucsf.edu.; Department of Medicine, Division of Infectious Diseases, University of California San Francisco, San Francisco, CA, USA. charles.chiu@ucsf.edu.

Publikováno v: Nature microbiology [Nat Microbiol] 2020 Mar; Vol. 5 (3), pp. 443-454. Date of Electronic Publication: 2020 Jan 13.