Výsledky vyhledávání

Adenosine 5'-0-[S-(4-succinimidyl-benzophenone)thiophosphate]: a new photoaffinity label of the allosteric ADP site of bovine liver glutamate dehydrogenase

Autor: K S, Madhusoodanan, R F, Colman

Publikováno v: Biochemistry. 40(6)

By reaction of adenosine 5'-monothiophosphate with benzophenone-4-maleimide, we synthesized adenosine 5'-O-[S-(4-succinimidyl-benzophenone)thiophosphate] (AMPS-Succ-BP) as a photoreactive ADP analogue. Bovine liver glutamate dehydrogenase is known to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::898ee023d372c4640d1d32009053e8b6
https://pubmed.ncbi.nlm.nih.gov/11327816

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Inactivation of platelet PDE2 by an affinity label: 8-[(4-bromo-2, 3-dioxobutyl)thio]cAMP

Autor: F, Mseeh, R F, Colman, R W, Colman

Publikováno v: Thrombosis research. 98(5)

Cyclic GMP-stimulated cyclic nucleotide phosphodiesterase (PDE2) is the second most abundant of this class of enzymes in platelets. PDE2 probably plays an important role in the regulation of elevated intracellular concentrations of cAMP and cGMP in p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b4d44c643fd740bb07ff1386470fa8f3
https://pubmed.ncbi.nlm.nih.gov/10828479

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Inactivation of recombinant monocyte cAMP-specific phosphodiesterase by cAMP analog, 8-[(4-bromo-2,3-dioxobutyl)thio]adenosine 3',5'-cyclic monophosphate

Autor: G A, Omburo, T J, Torphy, G, Scott, S, Jacobitz, R F, Colman, R W, Colman

Publikováno v: Blood. 89(3)

Two cAMP analogs, 8- and 2- [(4-bromo-2,3-dioxobutyl)thio]adenosine 3',5'-cyclic monophosphate (8- and 2-BDB-TcAMP) have been used in probing the catalytic site of recombinant monocyte cAMP-specific phosphodiesterase (PDE4a). 2-BDB-TcAMP is a reversi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::991ff93211bda3a8e6e7366c12e00937
https://pubmed.ncbi.nlm.nih.gov/9028334

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Identification of His141 in the active site of Bacillus subtilis adenylosuccinate lyase by affinity labeling with 6-(4-bromo-2,3-dioxobutyl)thioadenosine 5'-monophosphate

Autor: T T, Lee, C, Worby, J E, Dixon, R F, Colman

Publikováno v: The Journal of biological chemistry. 272(1)

Adenylosuccinate lyase of Bacillus subtilis is inactivated by 25-400 microM 6-(4-bromo-2,3-dioxobutyl)thioadenosine 5'-monophosphate (6-BDB-TAMP) at pH 7.0 and 25 degrees C. The initial inactivation rate constant exhibits nonlinear dependence on the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::26cc5514e1cbd7c54a1133145acadbe5
https://pubmed.ncbi.nlm.nih.gov/8995283

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Probing the active site of alpha-class rat liver glutathione S-transferases using affinity labeling by monobromobimane

Autor: L, Hu, B L, Borleske, R F, Colman

Publikováno v: Protein science : a publication of the Protein Society. 6(1)

Monobromobimane (mBBr) is a substrate of both mu- and alpha-class rat liver glutathione S-transferases, with Km values of 0.63 microM and 4.9 microM for the mu-class isozymes 3-3 and 4-4, respectively, and 26 microM for the alpha-class isozymes 1-1 a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::54844efbb46505f939b7320f3ad6425e
https://pubmed.ncbi.nlm.nih.gov/9007975

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Tyrosine 8 contributes to catalysis but is not required for activity of rat liver glutathione S-transferase, 1-1

Autor: J, Wang, J J, Barycki, R F, Colman

Publikováno v: Protein science : a publication of the Protein Society. 5(6)

Reaction of rat liver glutathione S-transferase, isozyme 1-1, with 4-(fluorosulfonyl)benzoic acid (4-FSB), a xenobiotic substrate analogue, results in a time-dependent inactivation of the enzyme to a final value of 35% of its original activity when a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b80c349f9a66a509bdd48046c18a7ac5
https://pubmed.ncbi.nlm.nih.gov/8762135

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Guanosine 5'-O-[S-(4-bromo-2,3-dioxobutyl)]thiophosphate and adenosine 5'-O-[S-(4-bromo-2,3-dioxobutyl)]thiophosphate. New nucleotide affinity labels which react with rabbit muscle pyruvate kinase

Autor: S H, Vollmer, M B, Walner, K V, Tarbell, R F, Colman

Publikováno v: The Journal of biological chemistry. 269(11)

Three new reactive nucleotide analogues with bromo-keto substituents adjacent to a thiophosphate have been synthesized. Guanosine 5'-O-[S-(4-bromo-2,3-dioxobutyl)]thiophosphate (GMPS-BDB), reacts covalently with rabbit muscle pyruvate kinase with com

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::967f14b26e339e1ccfa3abf598ec27e1
https://pubmed.ncbi.nlm.nih.gov/8132533

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