Zobrazeno 1 - 10
of 148
pro vyhledávání: '"R Edgell"'
Autor:
Dalton T. Ham, Tyler S. Browne, Pooja N. Banglorewala, Tyler L. Wilson, Richard K. Michael, Gregory B. Gloor, David R. Edgell
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract The CRISPR/Cas9 nuclease from Streptococcus pyogenes (SpCas9) can be used with single guide RNAs (sgRNAs) as a sequence-specific antimicrobial agent and as a genome-engineering tool. However, current bacterial sgRNA activity models struggle
Externí odkaz:
https://doaj.org/article/e91f69064291470d9447cda6db83c808
Autor:
Rachel D. Kelly, Gursimran Parmar, Laila Bayat, Matthew E. R. Maitland, Gilles A. Lajoie, David R. Edgell, Caroline Schild-Poulter
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-15 (2023)
Abstract The Ku70/80 heterodimer is a key player in non-homologous end-joining DNA repair but is involved in other cellular functions like telomere regulation and maintenance, in which Ku’s role is not fully characterized. It was previously reporte
Externí odkaz:
https://doaj.org/article/eb6a2b9fcb3446e0b036e18a1fd9df1c
Autor:
Samuel S. Slattery, Daniel J. Giguere, Emily E. Stuckless, Arina Shrestha, Lee-Ann K. Briere, Alexa Galbraith, Stephen Reaume, Xenia Boyko, Henry H. Say, Tyler S. Browne, Mallory I. Frederick, Jeremy T. Lant, Ilka U. Heinemann, Patrick O’Donoghue, Liann Dsouza, Steven Martin, Peter Howard, Christopher Jedeszko, Kinza Ali, Garth Styba, Martin Flatley, Bogumil J. Karas, Gregory B. Gloor, David R. Edgell
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)
Abstract The worldwide COVID-19 pandemic caused by the SARS-CoV-2 betacoronavirus has highlighted the need for a synthetic biology approach to create reliable and scalable sources of viral antigen for uses in diagnostics, therapeutics and basic biome
Externí odkaz:
https://doaj.org/article/addb994c28b44678acdeb227ced926e1
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
Externí odkaz:
https://doaj.org/article/98c6454a014f40ecad35b4895153c832
Autor:
Daniel J. Giguere, Alexander T. Bahcheli, Samuel S. Slattery, Rushali R. Patel, Tyler S. Browne, Martin Flatley, Bogumil J. Karas, David R. Edgell, Gregory B. Gloor
Publikováno v:
PeerJ, Vol 10, p e13607 (2022)
Phaeodactylum tricornutum is a marine diatom with a growing genetic toolbox available and is being used in many synthetic biology applications. While most of the genome has been assembled, the currently available genome assembly is not a completed te
Externí odkaz:
https://doaj.org/article/f232fb4cb60c41b98323c22c74f590df
Autor:
Ryan R. Cochrane, Arina Shrestha, Mariana M. Severo de Almeida, Michelle Agyare-Tabbi, Stephanie L. Brumwell, Samir Hamadache, Jordyn S. Meaney, Daniel P. Nucifora, Henry Heng Say, Jehoshua Sharma, Maximillian P. M. Soltysiak, Cheryl Tong, Katherine Van Belois, Emma J. L. Walker, Marc-André Lachance, Gregory B. Gloor, David R. Edgell, Rebecca S. Shapiro, Bogumil J. Karas
Publikováno v:
BioDesign Research, Vol 2022 (2022)
Fungi are nature’s recyclers, allowing for ecological nutrient cycling and, in turn, the continuation of life on Earth. Some fungi inhabit the human microbiome where they can provide health benefits, while others are opportunistic pathogens that ca
Externí odkaz:
https://doaj.org/article/3eae55de9a434a30ae09547dd4d83ec6
Autor:
Thomas A. Hamilton, Gregory M. Pellegrino, Jasmine A. Therrien, Dalton T. Ham, Peter C. Bartlett, Bogumil J. Karas, Gregory B. Gloor, David R. Edgell
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the con
Externí odkaz:
https://doaj.org/article/b1cfdfe24838427d8b5b49368d6df85b
Autor:
Gregory M Pellegrino, Tyler S Browne, Keerthana Sharath, Khaleda A Bari, Sarah J Vancuren, Emma Allen-Vercoe, Gregory B Gloor, David R Edgell
Publikováno v:
Nucleic Acids Research. 51:982-996
The ability to restrict gene expression to a relevant bacterial species in a complex microbiome is an unsolved problem. In the context of the human microbiome, one desirable target metabolic activity are glucuronide-utilization enzymes (GUS) that are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1c7977f27ee4f6fd767730d91b9020e4
https://doi.org/10.1093/nar/gkac1248
https://doi.org/10.1093/nar/gkac1248
Autor:
Claudia V Pereira, Sandra R Bacman, Tania Arguello, Ugne Zekonyte, Sion L Williams, David R Edgell, Carlos T Moraes
Publikováno v:
EMBO Molecular Medicine, Vol 10, Iss 9, Pp 1-11 (2018)
Abstract Pathogenic mitochondrial DNA (mtDNA) mutations often co‐exist with wild‐type molecules (mtDNA heteroplasmy). Phenotypes manifest when the percentage of mutant mtDNA is high (70–90%). Previously, our laboratory showed that mitochondria
Externí odkaz:
https://doaj.org/article/7a381d0d4b6e4d0eb16577d5be175b52
Autor:
Stephanie L Brumwell, Michael R MacLeod, Tony Huang, Ryan R Cochrane, Rebecca S Meaney, Maryam Zamani, Ola Matysiakiewicz, Kaitlyn N Dan, Preetam Janakirama, David R Edgell, Trevor C Charles, Turlough M Finan, Bogumil J Karas
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0206781 (2019)
Storage, manipulation and delivery of DNA fragments is crucial for synthetic biology applications, subsequently allowing organisms of interest to be engineered with genes or pathways to produce desirable phenotypes such as disease or drought resistan
Externí odkaz:
https://doaj.org/article/cea7a9fd87ad47e7b25e7a9700fc6982