Zobrazeno 1 - 10
of 18
pro vyhledávání: '"R E, Dalbey"'
Publikováno v:
Methods in enzymology. 584
Signal peptidases are membrane proteases that play crucial roles in the protein transport pathway of bacteria. They cleave off the signal peptide from precursor proteins that are membrane inserted by the SecYEG or Tat translocons. Signal peptide clea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c65686b445ef83d3d8cb5714b3633d2d
https://pubmed.ncbi.nlm.nih.gov/28065271
https://pubmed.ncbi.nlm.nih.gov/28065271
Autor:
R E, Dalbey
Publikováno v:
Molecular Microbiology. 5:2855-2860
The Escherichia coli leader peptidase has been vital for unravelling problems in membrane assembly and protein export. The role of this essential peptidase is to remove amino-terminal leader peptides from exported proteins after they have crossed the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6777747074b527022c74cf8828be5bc9
https://doi.org/10.1111/j.1365-2958.1991.tb01844.x
https://doi.org/10.1111/j.1365-2958.1991.tb01844.x
Publikováno v:
The Journal of biological chemistry. 276(37)
The membrane insertion of the Sec-independent M13 Procoat protein in bacteria requires the membrane electrochemical potential and the integral membrane protein YidC. We show here that YidC is involved in the translocation but not in the targeting of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ec33f07ca21dc085979a7cffc057a06b
https://pubmed.ncbi.nlm.nih.gov/11457858
https://pubmed.ncbi.nlm.nih.gov/11457858
Publikováno v:
Pharmacologytherapeutics. 87(1)
Type I signal peptidases are essential membrane-bound serine proteases that function to cleave the amino-terminal signal peptide extension from proteins that are translocated across biological membranes. The bacterial signal peptidases are unique ser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d54adde0bd23323a58b09cd116e1fde0
https://pubmed.ncbi.nlm.nih.gov/10924740
https://pubmed.ncbi.nlm.nih.gov/10924740
Publikováno v:
Protein science : a publication of the Protein Society. 6(6)
The discovery that proteins exported from the cytoplasm are typically synthesized as larger precursors with cleavable signal peptides has focused interest on the peptidases that remove the signal peptides. Here, we review the membrane-bound peptidase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::59db8451d55af7d28507326b1faac696
https://pubmed.ncbi.nlm.nih.gov/9194173
https://pubmed.ncbi.nlm.nih.gov/9194173
Publikováno v:
Journal of molecular biology. 245(4)
An effective method for the determination of the activity of signal peptidase I (SPase I) of Escherichia coli is established using the hybrid protein pro-OmpA-nuclease A as substrate. Pro-OmpA-nuclease A, a hybrid secretory precursor was purified to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::674842adc340d703cf7102c619771ab2
https://pubmed.ncbi.nlm.nih.gov/7837264
https://pubmed.ncbi.nlm.nih.gov/7837264
Publikováno v:
The Journal of biological chemistry. 269(43)
We have studied the membrane insertion of two proteins from the inner membrane of Escherichia coli, both with two transmembrane segments connected by a short periplasmic loop: the M13 procoat protein and a mutant "inverted" leader peptidase. Neither
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::91105b088d542c59cc2a46ae3367f720
https://pubmed.ncbi.nlm.nih.gov/7929429
https://pubmed.ncbi.nlm.nih.gov/7929429
Autor:
W R, Tschantz, R E, Dalbey
Publikováno v:
Methods in enzymology. 244
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0ca3bf123a5abd2d3d354c866722355e
https://pubmed.ncbi.nlm.nih.gov/7845215
https://pubmed.ncbi.nlm.nih.gov/7845215
Publikováno v:
The Journal of biological chemistry. 268(36)
We report that a thiol leader peptidase, produced by replacing the critical serine at position 90 with a cysteine residue, is enzymatically active. In contrast to the wild-type leader peptidase, the thiol enzyme can be inactivated with N-ethylmaleimi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::91f493b745dbb2e30dfe5fe5a861fd9f
https://pubmed.ncbi.nlm.nih.gov/8262975
https://pubmed.ncbi.nlm.nih.gov/8262975
Autor:
M, Sung, R E, Dalbey
Publikováno v:
The Journal of biological chemistry. 267(19)
Leader peptidase of Escherichia coli cleaves the leader sequence from the amino terminus of membrane and secreted proteins after these proteins insert across the membrane. Despite considerable research, the mechanism of catalysis of leader peptidase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0105d484ef9d42715caf5b76a3a2718e
https://pubmed.ncbi.nlm.nih.gov/1618816
https://pubmed.ncbi.nlm.nih.gov/1618816