Zobrazeno 1 - 10
of 30
pro vyhledávání: '"R D Sekura"'
Autor:
Yan ling Zhang, R. D. Sekura
Publikováno v:
Infection and Immunity. 59:3754-3759
A procedure is described for purification of pertussis heat-labile toxin (PEHLT) from cells of Bordetella pertussis. The purification procedure, performed in the cold and in the presence of protease inhibitors, gives 1,350-fold purification with yiel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fbf7d59928830366c7dda3025c1c5e4
https://doi.org/10.1128/iai.59.10.3754-3759.1991
https://doi.org/10.1128/iai.59.10.3754-3759.1991
Publikováno v:
Journal of Biological Chemistry. 259:8693-8698
The D-2 dopamine receptor mediates inhibition of adenylate cyclase in rat intermediate lobe; this receptor is linked to cyclase by the inhibitory guanyl nucleotide-binding protein (Ni). The functioning of components in the inhibitory system was compa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f4f0b3a9806fc7ab4a942b2f054c6705
https://doi.org/10.1016/s0021-9258(17)47207-3
https://doi.org/10.1016/s0021-9258(17)47207-3
Publikováno v:
Journal of Biological Chemistry. 261:9333-9340
Thyrotropin-releasing hormone (TRH) stimulated a rapid rise in inositol trisphosphate (IP3) formation and prolactin release from 7315c tumor cells. The potencies (half-maximal) of TRH in stimulating IP3 formation and prolactin release were 100 +/- 30
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e1d6dd31995bc1b252f4d31405063cfb
https://doi.org/10.1016/s0021-9258(18)67659-8
https://doi.org/10.1016/s0021-9258(18)67659-8
Publikováno v:
Journal of Biological Chemistry. 259:11408-11418
The effect of GTP analogs and Mg on the structure of Ns and Ni, the stimulatory and inhibitory regulatory components of adenylyl cyclase, were studied in a comparative manner. Both N proteins, which are alpha beta gamma heterotrimers that differ in t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::416052f247375ff9eccbfd0f7e391f1e
https://doi.org/10.1016/s0021-9258(18)90876-8
https://doi.org/10.1016/s0021-9258(18)90876-8
Publikováno v:
Journal of Biological Chemistry. 260:2585-2588
Both cholera toxin and pertussis toxin catalyzed ADP-ribosylation of purified bovine brain tubulin. The effect of cholera toxin was evident in the absence or presence of nucleotides. In contrast, pertussis toxin required adenine nucleotides for its A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::96e312a07a718ba4ccea2dccf3c9ff1e
https://doi.org/10.1016/s0021-9258(18)89395-4
https://doi.org/10.1016/s0021-9258(18)89395-4
Publikováno v:
Journal of Biological Chemistry. 261:11173-11179
The interaction of nucleotides with pertussis toxin (PT), and their effects on the ability of the toxin to ADP-ribosylate pure Ni, were evaluated. [32P]ATP (10 nM) bound directly to dithiothreitol-activated PT. This binding was competitively inhibite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c704cc81d6b6e780c61c81cdf27944a
https://doi.org/10.1016/s0021-9258(18)67364-8
https://doi.org/10.1016/s0021-9258(18)67364-8
Publikováno v:
Journal of Biological Chemistry. 262:11247-11251
The effect of the addition of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), the GTP analog which activates the inhibitory guanine nucleotide-binding regulatory protein of adenylyl cyclase (Ni), on the pertussis toxin-mediated ADP-ribosylation re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77900c486ad78f36e84e830287b40b5c
https://doi.org/10.1016/s0021-9258(18)60951-2
https://doi.org/10.1016/s0021-9258(18)60951-2
Publikováno v:
Journal of Biological Chemistry. 259:7893-7901
Treatment of MDCK cells with glucagon results in decreases in glucagon, NaF and prostaglandin E1-stimulated adenylyl cyclase activities, indicating the occurrence of a heterologous desensitization process. The extent of desensitization was time and g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::242b29692ecb151b2436ea4c21b5c904
https://doi.org/10.1016/s0021-9258(17)42877-8
https://doi.org/10.1016/s0021-9258(17)42877-8
Autor:
Charles R. Manclark, R D Sekura, Ravi Iyengar, John D. Hildebrandt, J Bryan, Mariel Birnbaumer, Lutz Birnbaumer, Juan Codina
Publikováno v:
Journal of Biological Chemistry. 259:5871-5886
Methods were developed to adequately extract, separate and, without the use of NaF as stabilizing agent, purify to better than 90% purity human erythrocyte Ns and Ni, the stimulatory and inhibitory guanine nucleotide- and Mg-binding regulatory compon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a07663ca93ffa7c2a5475063185d8441
https://doi.org/10.1016/s0021-9258(18)91097-5
https://doi.org/10.1016/s0021-9258(18)91097-5
Publikováno v:
Journal of Biological Chemistry. 261:4555-4561
Stimulation of the D-2 dopamine receptor inhibits pro-opiomelanocortin (POMC) synthesis in isolated rat intermediate lobe tissue. Intermediate lobe tissue was incubated in the absence or presence of various dopaminergic compounds, and then its capaci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5518b89df6e41e3825be1e05c596d0b3
https://doi.org/10.1016/s0021-9258(17)38537-x
https://doi.org/10.1016/s0021-9258(17)38537-x