Výsledky vyhledávání - "R Borlinghaus"

A modified confocal laser scanning microscope allows fast ultraviolet ratio imaging of intracellular Ca2+ activity using Fura-2

Autor: R. Borlinghaus, Roland Nitschke, S. Wilhelm, Rainer Greger, Jens Leipziger, R.J.M. Bindels

Publikováno v: Pflügers Archiv : European Journal of Physiology, 433, pp. 653-663
Pflügers Archiv : European Journal of Physiology, 433, 653-663
Pflügers Archiv : European Journal of Physiology, 433, 5, pp. 653-663
Pflügers Archiv : European Journal of Physiology, 433, 653-663. Springer verlag

A confocal, ultraviolet laser scanning microscope (LSM) for reliable ratio measurements of localized intracellular Ca2+ gradients using the Ca2+-sensitive dye Fura-2 was developed. In a commercial LSM, the filter wheels for the excitation band-pass f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84d85a92707345439e88ad516e849530
https://hdl.handle.net/2066/27891

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Direct identification, and localization of Azospirillum in the rhizosphere of wheat using fluorescence-labelled monoclonal antibodies and confocal scanning laser microscopy

Autor: W. Bode, R. Borlinghaus, M. Schloter, A. Hartmann

Publikováno v: Journal of Microscopy. 171:173-177

Summary Azospirillum is a soil bacterium with plant growth-promoting potential. Strain-specific monoclonal antibodies (mAbs) which bind to different antigenic determinants of the bacterial cell surface with high specificity for A. brasilense Sp7 were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::365d1a3f606676c3c9749dbc64ac288a
https://doi.org/10.1111/j.1365-2818.1993.tb03371.x

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Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin

Autor: Stephen F. Lincoln, A. D. Ward, W. H. Betts, R. Borlinghaus, R. F. Seamark, Ian J. Forbes, Peter D. Zalewski

Publikováno v: Chemistrybiology. 1(3)

Background : The transition metal Zn(II) is thought to regulate cell and tissue growth by enhancing mitosis (cell proliferation) and suppressing the counterbalancing process of apoptosis (gene-directed cell death). To investigate the role of Zn(II) f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1528c548c843ab218685be72b464dbda
https://pubmed.ncbi.nlm.nih.gov/9383385

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Ion Translocation by the Na,K-Pump: Correlation between Electrogenic Events and Conformational Transitions

Autor: I. Wuddel, Hans-Jürgen Apell, Peter Läuger, R. Borlinghaus, W. Stürmer

Publikováno v: Water and Ions in Biomolecular Systems ISBN: 9783034872553

The electrogenic properties of the Na,K-ATPase can be studied by correlating transient electrical events in the pump molecule with optically-detected conformational changes. Membrane fragments containing a high density of oriented ATPase molecules ca

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::769a7a284fdae8a5dcc043557ac34b71
https://doi.org/10.1007/978-3-0348-7253-9_18

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Current transients generated by the Na+K+-ATPase after an ATP concentration jump: dependence on sodium and ATP concentration

Autor: R Borlinghaus, H.-J Apell

Publikováno v: Biochimica et Biophysica Acta (BBA) - Biomembranes. 939:197-206

Planar membrane fragments containing a high density of oriented Na+/K+-ATPase molecules are bound to planar lipid bilayers. ATP is released in the aqueous solution within milliseconds from an inactive, photolabile precursor ('caged ATP') by an intens

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01254d71b3ae0baee17960d3f20e6843
https://doi.org/10.1016/0005-2736(88)90063-6

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Fast charge translocations associated with partial reactions of the Na,K-pump: II. Microscopic analysis of transient currents

Autor: Hans-Jürgen Apell, Peter Läuger, R. Borlinghaus

Publikováno v: The Journal of Membrane Biology. 97:179-191

Nonstationary pump currents which have been observed in K+-free Na+ media after activation of the Na,K-ATPase by an ATP-concentration jump (see the preceding paper) are analyzed on the basis of microscopic reaction models. It is shown that the behavi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8b2be578c097d4115fc38da5476c5f1
https://doi.org/10.1007/bf01869221

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Charge Translocation in a Single Turnover of the Na,K-pump

Autor: Hans-Jürgen Apell, R. Borlinghaus, Peter Läuger

Publikováno v: The Jerusalem Symposia on Quantum Chemistry and Biochemistry ISBN: 9789401078825

Charge translocation associated with partial reactions of the Na,K-pump may be studied by fast perturbation methods. After an ATP concentration-jump, transient currents can be recorded in a compound membrane system consisting of flat Na,K-ATPase memb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9707d5ea505d65234f94bff31ca2e027
https://doi.org/10.1007/978-94-009-3075-9_31

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Fast charge translocations associated with partial reactions of the Na,K-pump: I. Current and voltage transients after photochemical release of ATP

Autor: R. Borlinghaus, Hans-Jürgen Apell, Peter Läuger

Publikováno v: The Journal of membrane biology. 97(3)

Nonstationary electric currents are described which are generated by the Na,K-pump. Flat membrane sheets 0.2-1 micron in diameter containing a high density of oriented Na,K-ATPase molecules are bound to a planar lipid bilayer acting as a capacitive e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::599341a99528d55a0681dbcbf7a0c4f0
https://pubmed.ncbi.nlm.nih.gov/3041002

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