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pro vyhledávání: '"R A, Heinzen"'
Autor:
Ted Hackstadt, R A Heinzen
Publikováno v:
Scopus-Elsevier
Two DNA-binding proteins have been detected in Coxiella burnetii by southwestern (DNA-protein) blotting. One of these, termed Hq1, is enriched in the small cell variant stage of the developmental cycle and displays compositional and primary amino aci
Publikováno v:
Acta virologica. 36(1)
Methods have been developed for the rapid detection of C. burnetii by specific hybridization of labelled DNA probes to rickettsial plasmid DNA sequences present in clinical samples. One DNA probe detects all C. burnetii strains, while additional prob
Publikováno v:
Acta virologica. 35(6)
Coxiella burnetii isolates from different genomic groups contain restriction fragment polymorphisms that were easily distinguishable using pulsed field gradient electrophoresis (PFGE). Conversely, isolates that belong to the same genomic group yield
Cloning and functional expression of the Coxiella burnetii citrate synthase gene in Escherichia coli
Autor:
R A Heinzen, L P Mallavia
Publikováno v:
Infection and immunity. 55(4)
The citrate synthase gene from the obligate intracellular rickettsial parasite Coxiella burnetii was cloned and expressed in Escherichia coli. Transduction into E. coli with a C. burnetii gene library constructed in the cosmid vector pHK17 resulted i